Nudt13 (BC049948) Mouse Tagged ORF Clone

CAT#: MR204668

Nudt13 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 13 (cDNA clone MGC:58873 IMAGE:3666660)

Product Images

Circular map for MR204668

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nudt13
• Synonyms: 4933433B15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204668 representing BC049948
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGTATTGTAGAACATTTTTCAGGAGGAAATCTTTTGGGTGCTATAGGCTGCTGTCAACCTATG
  TCACTAAGGCACGATACTTATTTGAATTGAAGGAAGATGAAGAGGCCTGCAGGAAGGCTCAGAAGACAGG
  AGTGTTTTACCTCTTTCATGACCTGGATCCTTTGCTCCAGGCGTCAGGACATCGATACCTGGTGCCCCGG
  CTTAGCCGAGCAGAGTTGGAAGGGCTGCTGGGTAAGTTCGGACAGGATTCGCAAAGAATTGAAGATTCGG
  TGCTGGTTGGGTGCTCCGAGCAGCAGGAAGCATGGTTTGCTTTGGATCTAGGTCTGAAGAGTGCCTCCTC
  CAGCCGTGCCTCCTTGCCGAAATCTGAAATGGAAGCAGAGCTGGGAGGTTCTTTCATCAAGCTGAGGCAG
  GCTCTTTTCCAGCTGAACTCGGTGGACTCCTCCTTGCTGTTCACGGCTCAGGCTCTTCTCCGCTGGCATG
  ACGGTCATCAGTTCTGCAGCAAAATGATCACACTGGTGTCAGATGGGGCCCGCTGCCTACTTGCCCGCCA
  GAGTTCCTTTCCCAAGGGGTTATATTCTGCCCTGGCAGGTTTCTGTGACATAGGTGAAAGTGTGGAAGAA
  ACTGTCCATCGAGAAGTTGCGGAAGAGGTGGGCCTGGAAGTGGAAAACATACAGTACTCTGCGTCCCAGC
  ACTGGCCCTTTCCCAATAGCTCACTCATGATCGCTTGTCACGCAACCGTGAAACCAGGACACACAGAGAT
  TCAGGTGAACTTGAAGGAACTAGAGGCAGCCGCCTGGTTCAGTCTTGATGAGGTGACCACAGCCCTGAGG
  AGAAAGGGCTCCTTGGCTCTGCAGCCGAGTGAGGCTTCCCCACTGTTGCTGCCTCCCAAGTTAGCCATTG
  CCCACCACTTGATTAAGAAGTGGGTGGAGACGCGGAGCTGTTCTTCCCTGGCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204668 representing BC049948
  Red=Cloning site Green=Tags(s)
  
  MSLYCRTFFRRKSFGCYRLLSTYVTKARYLFELKEDEEACRKAQKTGVFYLFHDLDPLLQASGHRYLVPR
  LSRAELEGLLGKFGQDSQRIEDSVLVGCSEQQEAWFALDLGLKSASSSRASLPKSEMEAELGGSFIKLRQ
  ALFQLNSVDSSLLFTAQALLRWHDGHQFCSKMITLVSDGARCLLARQSSFPKGLYSALAGFCDIGESVEE
  TVHREVAEEVGLEVENIQYSASQHWPFPNSSLMIACHATVKPGHTEIQVNLKELEAAAWFSLDEVTTALR
  RKGSLALQPSEASPLLLPPKLAIAHHLIKKWVETRSCSSLAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049948
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC049948.1
• RefSeq Size: 1587 bp
• RefSeq ORF: 968 bp
• Locus ID: 67725
• Cytogenetics: 14 A3
• MW: 58.2 kDa
• Gene Summary: NAD(P)H pyrophosphatase that hydrolyzes NADH into NMNH and AMP, and NADPH into NMNH and 2',5'-ADP (PubMed:28755312). Has a marked preference for the reduced pyridine nucleotides (PubMed:28755312). Does not show activity toward NAD-capped RNAs; the NAD-cap is an atypical cap present at the 5'-end of some RNAs (PubMed:31101919).[UniProtKB/Swiss-Prot Function]