Bfar (NM_025653) Mouse Tagged ORF Clone

CAT#: MR204428

3110001I22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 3110001I22 gene (3110001I22Rik)

Product Images

Circular map for MR204428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bfar
• Synonyms: 3010001A07Rik; 3110001I22Rik; AI666707; AW107665; Bar; Rnf47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204428 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCATATCCCGGTCATCCTTGGTCACCGTCCAGGATCAGTGTACAGAAGAGACAAGATGTGGCCTA
  ACAGATCATATGATGAAGTACCTCAGAATGTCTCCAGTGATGAATCTACCCTGAGGAAAACAAGAGGTGA
  TCTTTCTGCAAGCCAACAGCATGAGTGCGGGGATGAGAAAGATGACTTTAGAAGAAACTGTTCCTCTTCT
  TCCCATTACTCAGAACAGCAGTCTCCCCATAAAGGGGCTTCTTGTTTTTCCAGAAAATCAGGTATAGATC
  AGAAGAACTCCCCACACAGCAGATCTGGATCCAGTGGAAGCCATTCTCCAAAACAAAGCAAACTGCATTC
  TTCCCATGGGTCAGAGCTTAGGAAGTCCGAGCATGCTCATTCCTCCTACAAACGGCACAGTGAAGAAAAG
  CCAGAAGGAGGTAAAAAGAGGGCTGGCCAGTCTTTGAAAACATCAAGAGACACACACCCAAAAAGTTCTT
  CAGCAGTTCCTTCGTCAGAAAGTTTAAACAAACTCAAAAGGCTTGATGAAAAGGGACGTCCTAAAGCTGC
  AAGCAGGCAAGCTGTAGAAAAGCCAATGATGGCAGATGAAAGTGACTTACCTAAAATTTCTGAGTTTGAA
  GTTGGATTCTCAGAACTTATGGATCAGCCACAAGAACCTGGGTCAAACAAAAAAGATCATACAGAATTCA
  TTGATGACCAAGTAACCAATCGCCGTAAAGCAATAGTGTCAAAGACCAAGGAAATTGAACAAGCCTACTA
  CCAAGACTGTGAGACCTTCGGAATGGTGGTGAAAATGCTGGTTGAAAAAGACGCTTCATTAGAAAGGCCC
  ATTCAGTTTGCGCTCCGGCAGAATTTACATGAACTAAGTGAGCGCTGTGTGGAGGAGCTCAGGCAGTTCA
  TTGCAGATTATGATGCTGCTGCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204428 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLHIPVILGHRPGSVYRRDKMWPNRSYDEVPQNVSSDESTLRKTRGDLSASQQHECGDEKDDFRRNCSSS
  SHYSEQQSPHKGASCFSRKSGIDQKNSPHSRSGSSGSHSPKQSKLHSSHGSELRKSEHAHSSYKRHSEEK
  PEGGKKRAGQSLKTSRDTHPKSSSAVPSSESLNKLKRLDEKGRPKAASRQAVEKPMMADESDLPKISEFE
  VGFSELMDQPQEPGSNKKDHTEFIDDQVTNRRKAIVSKTKEIEQAYYQDCETFGMVVKMLVEKDASLERP
  IQFALRQNLHELSERCVEELRQFIADYDAAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025653
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025653.3
• RefSeq Size: 1611 bp
• RefSeq ORF: 939 bp
• Locus ID: 67118
• Cytogenetics: 16 A1
• MW: 35.2 kDa
• Gene Summary: Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors (By similarity).[UniProtKB/Swiss-Prot Function]