Klf5 (BC006646) Mouse Tagged ORF Clone
CAT#: MR204381
- TrueORF®
Klf5 (Myc-DDK-tagged) - Mouse Kruppel-like factor 5 (cDNA clone MGC:7725 IMAGE:3498207)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC006646" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Klf5 |
Synonyms | 4930520J07Rik; Bteb2; CKLF; IKLF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204381 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATCTGACCCCTCAGCTCCCTCCAGTTCCGATAATTTCAGAGCATAAAAAGTATAGACGAG ACAGTGCCTCAGTGGTAGACCAGTTCTTCACTGACACTGAAGGCATACCTTACAGCATCAACATGAACGT CTTCCTCCCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCATGCGTAACACAGATC AAGACAGAACCTGTTACCATTTTCAGCCACCAGAGCGAGTCGACGGCCCCTCCTCCTCCTCCGGCCCCCA CCCAGGCTCTCCCCGAGTTCACTAGTATCTTCAGCTCCCACCAGACCACAGCGCCACCACAGGAGGTGAA CAATATCTTCATCAAACAAGAACTTCCTATACCAGATCTTCATCTCTCTGTCCCTTCCCAGCAGGGCCAC CTGTACCAGCTGTTGAATACACCGGATCTAGACATGCCCAGTTCGACAAACCAGACGGCAGTAATGGACA CCCTTAATGTTTCTATGGCAGGCCTTAACCCACACCCCTCTGCTGTTCCACAGACGTCAATGAAACAGTT CCAGGGCATGCCCCCTTGCACGTACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCCC CCGTCACCACCAAGCTCAGAGCCTGGAAGTCCCGATAGACAAGCTGAGATGCTGCAGAATCTCACCCCAC CTCCGTCCTATGCCGCTACAATTGCTTCCAAACTGGCGATTCACAACCCAAATTTACCTGCCACTCTGCC AGTTAATTCGCCAACTCTCCCACCTGTCAGATACAACAGAAGGAGTAACCCGGATCTGGAGAAGCGACGT ATCCACTTCTGCGATTATAATGGTTGCACAAAAGTTTATACAAAGTCGTCTCACTTAAAAGCTCACCTGA GGACTCATACGGGTAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204381 protein sequence
Red=Cloning site Green=Tags(s) MEKYLTPQLPPVPIISEHKKYRRDSASVVDQFFTDTEGIPYSINMNVFLPDITHLRTGLYKSQRPCVTQI KTEPVTIFSHQSESTAPPPPPAPTQALPEFTSIFSSHQTTAPPQEVNNIFIKQELPIPDLHLSVPSQQGH LYQLLNTPDLDMPSSTNQTAVMDTLNVSMAGLNPHPSAVPQTSMKQFQGMPPCTYTMPSQFLPQQATYFP PSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPATLPVNSPTLPPVRYNRRSNPDLEKRR IHFCDYNGCTKVYTKSSHLKAHLRTHTGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC006646 |
ORF Size | 927 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC006646, AAH06646 |
RefSeq Size | 2907 bp |
RefSeq ORF | 929 bp |
Locus ID | 12224 |
Cytogenetics | 14 E2.2 |
MW | 34.4 kDa |
Gene Summary | Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204381 | Klf5 (tGFP-tagged) - Mouse Kruppel-like factor 5 (cDNA clone MGC:7725 IMAGE:3498207) |
USD 500.00 |
|
MR204381L3 | Lenti ORF clone of Klf5 (Myc-DDK-tagged) - Mouse Kruppel-like factor 5 (cDNA clone MGC:7725 IMAGE:3498207) |
USD 600.00 |
|
MR204381L4 | Lenti ORF clone of Klf5 (mGFP-tagged) - Mouse Kruppel-like factor 5 (cDNA clone MGC:7725 IMAGE:3498207) |
USD 600.00 |
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