Klf5 (BC006646) Mouse Tagged ORF Clone

CAT#: MR204381

Klf5 (Myc-DDK-tagged) - Mouse Kruppel-like factor 5 (cDNA clone MGC:7725 IMAGE:3498207)

Product Images

Circular map for MR204381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klf5
• Synonyms: 4930520J07Rik; Bteb2; CKLF; IKLF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204381 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGTATCTGACCCCTCAGCTCCCTCCAGTTCCGATAATTTCAGAGCATAAAAAGTATAGACGAG
  ACAGTGCCTCAGTGGTAGACCAGTTCTTCACTGACACTGAAGGCATACCTTACAGCATCAACATGAACGT
  CTTCCTCCCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCATGCGTAACACAGATC
  AAGACAGAACCTGTTACCATTTTCAGCCACCAGAGCGAGTCGACGGCCCCTCCTCCTCCTCCGGCCCCCA
  CCCAGGCTCTCCCCGAGTTCACTAGTATCTTCAGCTCCCACCAGACCACAGCGCCACCACAGGAGGTGAA
  CAATATCTTCATCAAACAAGAACTTCCTATACCAGATCTTCATCTCTCTGTCCCTTCCCAGCAGGGCCAC
  CTGTACCAGCTGTTGAATACACCGGATCTAGACATGCCCAGTTCGACAAACCAGACGGCAGTAATGGACA
  CCCTTAATGTTTCTATGGCAGGCCTTAACCCACACCCCTCTGCTGTTCCACAGACGTCAATGAAACAGTT
  CCAGGGCATGCCCCCTTGCACGTACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCCC
  CCGTCACCACCAAGCTCAGAGCCTGGAAGTCCCGATAGACAAGCTGAGATGCTGCAGAATCTCACCCCAC
  CTCCGTCCTATGCCGCTACAATTGCTTCCAAACTGGCGATTCACAACCCAAATTTACCTGCCACTCTGCC
  AGTTAATTCGCCAACTCTCCCACCTGTCAGATACAACAGAAGGAGTAACCCGGATCTGGAGAAGCGACGT
  ATCCACTTCTGCGATTATAATGGTTGCACAAAAGTTTATACAAAGTCGTCTCACTTAAAAGCTCACCTGA
  GGACTCATACGGGTAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204381 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKYLTPQLPPVPIISEHKKYRRDSASVVDQFFTDTEGIPYSINMNVFLPDITHLRTGLYKSQRPCVTQI
  KTEPVTIFSHQSESTAPPPPPAPTQALPEFTSIFSSHQTTAPPQEVNNIFIKQELPIPDLHLSVPSQQGH
  LYQLLNTPDLDMPSSTNQTAVMDTLNVSMAGLNPHPSAVPQTSMKQFQGMPPCTYTMPSQFLPQQATYFP
  PSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPATLPVNSPTLPPVRYNRRSNPDLEKRR
  IHFCDYNGCTKVYTKSSHLKAHLRTHTGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006646
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006646, AAH06646
• RefSeq Size: 2907 bp
• RefSeq ORF: 929 bp
• Locus ID: 12224
• Cytogenetics: 14 E2.2
• MW: 34.4 kDa
• Gene Summary: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.[UniProtKB/Swiss-Prot Function]