Tecr (NM_134118) Mouse Tagged ORF Clone

CAT#: MR204349

Tecr (Myc-DDK-tagged) - Mouse trans-2,3-enoyl-CoA reductase (Tecr), transcript variant 1

Product Images

Circular map for MR204349

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tecr
• Synonyms: 2410016D23Rik; A230102P12Rik; AI173355; D17Ertd178e; Gpsn2; SC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204349 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCACTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAG
  AGCCTCAGGCCACCATTTCTGAAATCAAGACCCTTTTCACCAAGACACACCCGCAGTGGTATCCTGCCCG
  CCAGTCCCTCCGCCTGGACCCCAAGGGGAAGTCCCTGAAAGATGAAGATGTCTTACAGAAGCTTCCTGTG
  GGCACCACAGCCACACTCTACTTCCGGGACCTCGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTGACGG
  AGTATGCCGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGGGTACCCTTCATTTATGGCCGCAAATA
  CGACTTTACGTCCAGTCGGCATACGGTGGTGCACCTCGCCTGCATGTGCCACTCGTTCCACTACATCAAG
  CGCCTGCTGGAGACTCTCTTCGTGCACCGATTCTCTCACGGAACCATGCCTTTGCGAAACATCTTCAAAA
  ACTGCACCTACTATTGGGGCTTTGCTGCATGGATGGCTTATTACATCAACCACCCTCTCTACACACCCCC
  TACCTATGGAGTTCAGCAGGTTAAGCTGGCACTGGCCGTTTTTGTGATCTGCCAGCTTGGGAACTTCTCC
  ATCCACATGGCTCTTCGGGACCTTCGGCCTGCTGGGTCGAAAACCAGGAAGATCCCATACCCCACCAAGA
  ACCCCTTCACCTGGCTGTTCCTGTTGGTGTCCTGTCCCAACTACACTTATGAGGTGGGCTCCTGGATTGG
  CTTTGCCATCTTGACTCAGTGTGTCCCAGTGGCCCTCTTCTCCCTGGTGGGCTTCACCCAGATGACTATC
  TGGGCCAAGGGCAAACACCGCAGCTACCTGAAGGAGTTCCGCGACTACCCGCCCCTGCGCATGCCCATTA
  TTCCCTTCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204349 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKHYEVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV
  GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIK
  RLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFS
  IHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTI
  WAKGKHRSYLKEFRDYPPLRMPIIPFLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134118
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134118.5, NP_598879.1
• RefSeq Size: 1190 bp
• RefSeq ORF: 927 bp
• Locus ID: 106529
• UniProt ID: Q9CY27
• Cytogenetics: 8 40.22 cM
• MW: 36.1 kDa
• Gene Summary: Involved in both the production of very long-chain fatty acids for sphingolipid synthesis and the degradation of the sphingosine moiety in sphingolipids through the sphingosine 1-phosphate metabolic pathway (By similarity). Catalyzes the last of the four reactions of the long-chain fatty acids elongation cycle (By similarity). This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle (By similarity). This enzyme reduces the trans-2,3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be further elongated by entering a new cycle of elongation (By similarity). Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity). Catalyzes the saturation step of the sphingosine 1-phosphate metabolic pathway, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function]