Vsig8 (BC064106) Mouse Tagged ORF Clone

CAT#: MR204274

Vsig8 (Myc-DDK-tagged) - Mouse V-set and immunoglobulin domain containing 8 (cDNA clone MGC:73808 IMAGE:1528324)

Product Images

Circular map for MR204274

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vsig8
• Synonyms: A030011M19; EG240916
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204274 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGCAGGTATCTGACACAGCAACCTATGAGTGCCGGGTGAAGAAGACCACCATGGCCACCAGGA
  AGGTCATTGTCACTGTCCAAGCACGTCCTGCGGTGCCCATGTGTTGGACGGAAGGCCACATGTCAAAGGG
  CAACGATGTGGTGCTGAAGTGCTTTGCCAACGGAGGCTCTCAGCCCCTCTCCTACAAGTGGGCCAAGATC
  AGTGGGCACAGTCACCCCTACCGAGCTGGGGCTTACCACTCACAGCACAGCTTCCACTCTGAGCTTTCTT
  ACCAAGAGTCATTCCACAGCACCATCAACCAAGGCCTGGGCAACGGAGACCTGCTGTTGAAGGGCATCAA
  CGCAGACGACGATGGGCTGTATCAGTGCACAGTGGCCAACCATGTGGGCTACAGCGTCTGTGTGGTAGAG
  GTGAAAGTCTCAGACTCCCAGCGAGTAGGCATGATCGTTGGAGCAGTGCTGGGCTCTTTGCTCATGCTGG
  CCTGCCTGGCACTAGGCATCTGGGGGCTCATCTGCTGCTGCTGCGGAGGCGGCGGGGCCGGTGGTGCCCG
  AGGTGCCTTCGGCTACGGGGTCGGCGGCGGGGTCGGCGGAGGGGCCTGCGGCGACTTGGCTAGTGAGATC
  AGAGTGGACGCCGAGGCGCCCGGGTGTAAGGCCAGCGGGCGCGGCAGCCGCGTCACCCACCTCCTGGGGT
  ACCCGACGCAGAACGTCAGCCGCTCCCTGCGCCGCAAGTACGCGCCTCCGCCCTGCGGCGGCCCCGAGGA
  CGTGGCCCTAGTGCCCCGCACCGCCTCCGCCTCCTGCGAAGCGGGTCCCTCCCCCGTCTACATCAAGGTC
  AAGAGCGCGGAGCCGGCCGACTGCGCCGACTGTGCCCAGGTCGAGCAGCGCTCGTGCAAGGACGGCCTCT
  TAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204274 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLQVSDTATYECRVKKTTMATRKVIVTVQARPAVPMCWTEGHMSKGNDVVLKCFANGGSQPLSYKWAKI
  SGHSHPYRAGAYHSQHSFHSELSYQESFHSTINQGLGNGDLLLKGINADDDGLYQCTVANHVGYSVCVVE
  VKVSDSQRVGMIVGAVLGSLLMLACLALGIWGLICCCCGGGGAGGARGAFGYGVGGGVGGGACGDLASEI
  RVDAEAPGCKASGRGSRVTHLLGYPTQNVSRSLRRKYAPPPCGGPEDVALVPRTASASCEAGPSPVYIKV
  KSAEPADCADCAQVEQRSCKDGLLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC064106
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC064106, AAH64106
• RefSeq Size: 1552 bp
• RefSeq ORF: 917 bp
• Locus ID: 240916
• Cytogenetics: 1 H3
• MW: 31.8 kDa