Tmem25 (BC060057) Mouse Tagged ORF Clone

CAT#: MR203767

Tmem25 (Myc-DDK-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102)

Product Images

Circular map for MR203767

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem25
• Synonyms: 0610039J01Rik; AI429491
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203767 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTGCCTCTAAGCCAAGCTACCCTCCGGCACACACTGCTGCTCCTTCCTGCCCTCTTAAGTTCAG
  GTCAAGGGGAGCTGGCGCCGCAAATTGATGGTCAGACCTGGGCTGAGAGAGCGCTTAGGGAGAATGAGCA
  CCATGCCTTCACCTGTCGGGTTGCAGGAGGTTCTGCCACTCCCCGATTAGCCTGGTACCTGGATGGACAG
  CTACAGGAGGCCACCACCTCCCGACTGCTGAGTGTGGGTGGGGACGCCTTCTCTGGAGGAACCAGCACTT
  TCACTGTTACTGCCCAGCGCTCCCAACATGAGCTTAACTGCTCCCTGCAGGACCCAGGGAGTGGCCGGCC
  AGCCAATGCCTCTGTCATCCTCAATGTGCAATTCAAACCAGAGATTGCCCAGGTTGGAGCCAAATACCAG
  GAAGCTCAGGGCCCAGGCCTTCTGGTTGTCCTGTTTGCCCTGGTGCGGGCCAACCCACCTGCCAATGTCA
  CCTGGATTGACCAGGATGGGCCAGTGACTGTCAATGCCTCTGACTTCCTGGTGCTGGATGCTCAGAACTA
  TCCCTGGCTCACCAACCACACTGTGCAGCTGCAGCTCCGGAGCTTGGCACACAACCTCTCGGTGGTGGCC
  ACCAATGACGTGGGTGTCACTAGCGCTTCCCTTCCAGCTCCAGGGCTCCTGGCCACACGGATAGAAGTGC
  CACTGCTGGGCATCGTTGTGGCTGGAGGACTTGCCTTGGGCACTCTAGTGGGGTTCAGTACCTTGGTGGC
  CTGCCTGGTCTGCAGGAAAGAGAAGAAAACAAAAGGCCCCTCCCGGCGTCCTTCTCTGATATCTAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203767 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELPLSQATLRHTLLLLPALLSSGQGELAPQIDGQTWAERALRENEHHAFTCRVAGGSATPRLAWYLDGQ
  LQEATTSRLLSVGGDAFSGGTSTFTVTAQRSQHELNCSLQDPGSGRPANASVILNVQFKPEIAQVGAKYQ
  EAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNASDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVA
  TNDVGVTSASLPAPGLLATRIEVPLLGIVVAGGLALGTLVGFSTLVACLVCRKEKKTKGPSRRPSLISR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060057
• ORF Size: 837 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC060057, AAH60057
• RefSeq Size: 2714 bp
• RefSeq ORF: 839 bp
• Locus ID: 71687
• Cytogenetics: 9 A5.2
• MW: 29.6 kDa
• Gene Summary: In neurons, modulates the degradation of NMDA receptor GRIN2B subunit. Plays a role in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]