Gtf2f2 (NM_026816) Mouse Tagged ORF Clone

CAT#: MR203175

Gtf2f2 (Myc-DDK-tagged) - Mouse general transcription factor IIF, polypeptide 2 (Gtf2f2)

Product Images

Circular map for MR203175

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gtf2f2
• Synonyms: 1110031C13Rik; AI326315
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203175 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGCGGGGAACTCGATCTGACGGGCGCCAAACAGAACACCGGCGTGTGGCTGGTCAAGGTTC
  CTAAATATTTGTCCCAGCAATGGGCAAAAGCCTCTGGAAGAGGTGAAGTTGGGAAACTGAGGATTGCCAA
  AAATCAAGGAAGGACTGAGGTATCATTTACTTTGAACGAGGATCTTGCAAATATTCATGACATCGGTGGG
  AAACCAGCTTCAGTCAGTGCTCCTAGAGAACACCCATTTGTCTTGCAAAGTGTTGGCGGACAGACATTAA
  CAGTGTTTACGGAGAGCTCCTCAGATAAACTGTCATTGGAAGGAATAGTGGTGCAGAGAGCGGAATGCCG
  GCCTGCTGCCAGTGAGAACTACATGAAATTAAAGAGATTGCAAATAGAAGAATCTTCGAAACCTGTAAGG
  CTCTCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAGCCTGTTGCTAACCATCAGTACAATATTG
  AATATGAAAGGAAGAAGAAAGAGGATGGAAAGCGAGCTCGGGCAGACAAACAACATGTCCTAGACATGCT
  GTTCTCTGCCTTTGAGAAGCATCAGTACTATAATCTCAAGGACTTGGTGGACATCACAAAGCAGCCTGTG
  GGATACCTGAAAGAAATCTTGAAAGAAATTGGCATTCAGAATGTAAAAGGAATTCACAAGAACACATGGG
  AGCTGAAGCCAGAGTACAGGCATTACCAAACAGAAGAGAAGAGCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203175 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKNQGRTEVSFTLNEDLANIHDIGG
  KPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVR
  LSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPV
  GYLKEILKEIGIQNVKGIHKNTWELKPEYRHYQTEEKSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026816
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026816.1
• RefSeq Size: 1467 bp
• RefSeq ORF: 750 bp
• Locus ID: 68705
• UniProt ID: Q8R0A0
• Cytogenetics: 14 D3
• MW: 28.4 kDa
• Gene Summary: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity (By similarity).[UniProtKB/Swiss-Prot Function]