Emg1 (NM_013536) Mouse Tagged ORF Clone

CAT#: MR203055

Emg1 (Myc-DDK-tagged) - Mouse EMG1 nucleolar protein homolog (S. cerevisiae) (Emg1)

Product Images

Circular map for MR203055

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Emg1
• Synonyms: C2f; Grcc2f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203055 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGGCCAGTGGTGGCTTCCAACCTCGTGAGCGGCGATTTTCAGTGCAGGAGCAGGACTGGGAGA
  CTACGCCGCCTAAGAAGCTCCGGCTTGGGGCAGGAAGCAAGTGCGGAGGCCGGAGGCTCATTGTGGTGCT
  GGAAGGGGCCAGTCTGGAGACAGTCAAGGTAGGGAAAACTTACGAGCTACTCAACTGTGACAGGCACAAG
  TCCATGTTGTTGAAGAATGGACGGGACCCAGGGGAAGTCAGACCAGACATCACCCACCAGAGCCTGCTGA
  TGCTTATGGACAGCCCCCTGAACCGAGCTGGCTTGCTACAGGTTTACATCCACACACAGAAGAACGTGCT
  GATTGAAGTGAACCCCCAGACTCGAATTCCTAGAACCTTTGACCGATTTTGTGGCCTCATGGTTCAGCTT
  TTACACAAACTGAGCGTCCGAGCAGCCGACGGCCCTCAGAAGCTATTGAAGGTAATTAAGAATCCAGTGT
  CCGACCACTTCCCAGTTGGCTGTATGAAAATTGGCACTTCCTTTTCTGTTGAAGACATCAGTGACATTCG
  AGAGTTGGTGCCCAGTAGTGACCCAGTTGTGTTTGTGGTGGGGGCCTTTGCCCATGGCAAGGTCAGTGTG
  GAGTACACAGAAAAGATGGTGTCCATCAGCAACTATCCACTCTCTGCTGCGCTTACCTGTGCTAAAGTCA
  CCACAGCTTTTGAAGAAGTATGGGGTGTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203055 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAASGGFQPRERRFSVQEQDWETTPPKKLRLGAGSKCGGRRLIVVLEGASLETVKVGKTYELLNCDRHK
  SMLLKNGRDPGEVRPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQL
  LHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKIGTSFSVEDISDIRELVPSSDPVVFVVGAFAHGKVSV
  EYTEKMVSISNYPLSAALTCAKVTTAFEEVWGVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013536
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013536.2
• RefSeq Size: 1086 bp
• RefSeq ORF: 735 bp
• Locus ID: 14791
• UniProt ID: O35130
• Cytogenetics: 6 59.17 cM
• MW: 27 kDa
• Gene Summary: S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.[UniProtKB/Swiss-Prot Function]