Tmem127 (NM_175145) Mouse Tagged ORF Clone
CAT#: MR202916
- TrueORF®
Tmem127 (Myc-DDK-tagged) - Mouse transmembrane protein 127 (Tmem127)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_175145" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tmem127 |
Synonyms | 2310003P10Rik; AI314202; AI317350 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202916 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGCCCCTGGAGGTGCCGGGCTGCCGGGAGGGCGCCGGCGGAGAAGCCCGGGAAGCAGCGCTCTTC CCAAGCAGCCGGAGCGGAGCCTGGCCTCGGCCCTGCCTGGCGCCCTGTCCATCACAGCGCTGTGCACTGC CCTCGCGGAGCCCGCTTGGTTGCACATCCATGGAGGCACCTGTTCCCGCCAGGAGCTGGGGGTCTCGGAC GTGCTGGGCTATGTAAACCCGGATCTGCTAAAAGATTTCTGCATGAACCCCCAGACAGTGCTGCTCCTGA GGGTCATCGCTGCTTTCTGCTTCCTGGGCATCCTGTGTAGTCTCTCTGCATTCCTTTTGGATGTCTTTGG GCCCAAGCATCCAGCTCTGAAGATTACTCGTCGGTATGCCTTTGCTCACATCCTCACTGTCCTACAGTGT GCTACCGTCATTGGCTTTTCCTATTGGGCTTCTGAGCTCATCCTGGCCCAGCAGCAACAGCATAAGAAGT ACCACGGCTCCCAAGTCTATGTCACCTTTGCTGTTAGCTTCTACTTGGTGGCAGGAGCTGGTGGAGCCTC AATCCTGGCTACTGCAGCCAACCTCCTGCGCCACTACCCAACAGAGGAAGAAGAACAAGCACTGGAGCTA CTATCTGAGATGGAGGAGAATGACCCCTACCCAGCAGAGTATGAGGTCATCAACCAATTCCAGCCACCCC CTGCCTATACACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202916 protein sequence
Red=Cloning site Green=Tags(s) MYAPGGAGLPGGRRRRSPGSSALPKQPERSLASALPGALSITALCTALAEPAWLHIHGGTCSRQELGVSD VLGYVNPDLLKDFCMNPQTVLLLRVIAAFCFLGILCSLSAFLLDVFGPKHPALKITRRYAFAHILTVLQC ATVIGFSYWASELILAQQQQHKKYHGSQVYVTFAVSFYLVAGAGGASILATAANLLRHYPTEEEEQALEL LSEMEENDPYPAEYEVINQFQPPPAYTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175145 |
ORF Size | 717 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175145.4 |
RefSeq Size | 4401 bp |
RefSeq ORF | 717 bp |
Locus ID | 69470 |
UniProt ID | Q8BGP5 |
Cytogenetics | 2 61.87 cM |
MW | 25.8 kDa |
Gene Summary | Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207684 | Tmem127 (untagged) - Mouse transmembrane protein 127 (Tmem127), (10ug) |
USD 330.00 |
|
MG202916 | Tmem127 (tGFP-tagged) - Mouse transmembrane protein 127 (Tmem127) |
USD 500.00 |
|
MR202916L3 | Lenti ORF clone of Tmem127 (Myc-DDK-tagged) - Mouse transmembrane protein 127 (Tmem127) |
USD 600.00 |
|
MR202916L4 | Lenti ORF clone of Tmem127 (mGFP-tagged) - Mouse transmembrane protein 127 (Tmem127) |
USD 600.00 |
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