Hacd4 (NM_025760) Mouse Tagged ORF Clone
CAT#: MR202800
- TrueORF®
Ptplad2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase-like A domain containing 2 (Ptplad2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025760" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hacd4 |
Synonyms | 4933428I03Rik; Hcad4; Ptplad2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202800 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCTCGGTGCTGCCAGCCTGGCTGCAGCCCAGGTATAGGAAGAATGTATATCTTTTCATCTATT ACTTAATTCAGTTCTGTGGCCACTCATGGATACTGGCAAATATGACAGTCAGATTCTTCTCATTTGGGAA AGATTCCATGGCTGACACGTTTTACGCTATTGGGTTAGTTATGCGGGTTTGCCAATCCATTTCCCTTTTG GAGCTTCTGCACATTTACATTGGCATTGAGTCGAATCAGCTTTTCCCACGGTTTTTGCAGCTCACAGAGA GAGTGATCATTCTCTTTGGGGTGATCACCAGTCAAGAGGAGGTCCAAGAGAAATGTGTAGTGTGTGTTTT ATTCATCCTTTGGAATCTACTGGATATGGTAAGGTACACTTACAGCATGCTGTCAGTCATAGGGACATCT TATGCTGCCTTGACATGGCTCAGTCAAACACTCTGGATGCCAATTTACCCTCTGTGTGTTCTTGCTGAAG CATTCACCATCTATCAGTCACTGCCTTATTTTGAGTCATTTGGTACAAACTCCACCGTGCTTCCGTTCGA CCTATCTACCTGTTTCCCTTATGTGCTGAAGCTGTACCTCATGATGCTCTTCATAGGTATGTATTTCACC TACAGCCATCTTTATACAGAAAGAAAAGACTTCCTCCGAGTTTTTTCTGTCAAACAGAAGAACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202800 protein sequence
Red=Cloning site Green=Tags(s) MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLL ELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTS YAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFT YSHLYTERKDFLRVFSVKQKNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_025760 |
ORF Size | 699 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025760.1, NM_025760.2, NM_025760.3, NM_025760.4, NP_080036.1 |
RefSeq Size | 2089 bp |
RefSeq ORF | 699 bp |
Locus ID | 66775 |
UniProt ID | A2AKM2 |
Cytogenetics | 4 C4 |
MW | 27.2 kDa |
Gene Summary | Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203114 | Ptplad2 (untagged) - Mouse protein tyrosine phosphatase-like A domain containing 2 (Ptplad2), (10ug) |
USD 300.00 |
|
MG202800 | Ptplad2 (tGFP-tagged) - Mouse protein tyrosine phosphatase-like A domain containing 2 (Ptplad2) |
USD 500.00 |
|
MR202800L3 | Lenti ORF clone of Ptplad2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase-like A domain containing 2 (Ptplad2) |
USD 600.00 |
|
MR202800L4 | Lenti ORF clone of Ptplad2 (mGFP-tagged) - Mouse protein tyrosine phosphatase-like A domain containing 2 (Ptplad2) |
USD 600.00 |
{0} Product Review(s)
Be the first one to submit a review