Gcg (NM_008100) Mouse Tagged ORF Clone
CAT#: MR201620
- TrueORF®
Gcg (Myc-DDK-tagged) - Mouse glucagon (Gcg)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008100" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Gcg |
Synonyms | Gl; GLP; GLP-1; Glu; P; PPG |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201620 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACCATTTACTTTGTGGCTGGATTGCTTATAATGCTGGTGCAAGGCAGCTGGCAGCACGCCCTTC AAGACACAGAGGAGAACCCCAGATCATTCCCAGCTTCCCAGACAGAAGCGCATGAGGACCCTGATGAGAT GAATGAAGACAAACGCCACTCACAGGGCACATTCACCAGCGACTACAGCAAATACCTGGACTCCCGCCGT GCCCAAGATTTTGTGCAGTGGTTGATGAACACCAAGAGGAACCGGAACAACATTGCCAAACGTCATGATG AATTTGAGAGGCATGCTGAAGGGACCTTTACCAGTGATGTGAGTTCTTACTTGGAGGGCCAGGCAGCAAA GGAATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGACTTCCCAGAAGAAGTCGCCATTGCCGAG GAACTCGGCCGCAGGCACGCTGATGGCTCCTTCTCTGACGAGATGAGCACCATTCTGGATAATCTTGCCA CCAGGGACTTCATCAACTGGCTGATTCAAACCAAGATCACTGACAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201620 protein sequence
Red=Cloning site Green=Tags(s) MKTIYFVAGLLIMLVQGSWQHALQDTEENPRSFPASQTEAHEDPDEMNEDKRHSQGTFTSDYSKYLDSRR AQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIAE ELGRRHADGSFSDEMSTILDNLATRDFINWLIQTKITDKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008100 |
ORF Size | 543 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008100.4 |
RefSeq Size | 1091 bp |
RefSeq ORF | 543 bp |
Locus ID | 14526 |
UniProt ID | P55095 |
Cytogenetics | 2 35.85 cM |
MW | 20.9 kDa |
Gene Summary | This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200864 | Gcg (untagged) - Mouse glucagon (Gcg), (10ug) |
USD 300.00 |
|
MG201620 | Gcg (tGFP-tagged) - Mouse glucagon (Gcg) |
USD 500.00 |
|
MR201620L3 | Lenti ORF clone of Gcg (Myc-DDK-tagged) - Mouse glucagon (Gcg) |
USD 600.00 |
|
MR201620L4 | Lenti ORF clone of Gcg (mGFP-tagged) - Mouse glucagon (Gcg) |
USD 600.00 |
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