Ppih (NM_028677) Mouse Tagged ORF Clone
CAT#: MR201558
- TrueORF®
Ppih (Myc-DDK-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_028677" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ppih |
Synonyms | 1100001J08Rik; 2010111B15Rik; 4833408F11Rik; AI464484; CYP-20; CYPH; D4Wsu43e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201558 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGCAAATTCAAGTCCAGTCAATCCAGTGGTCTTCTTCGATGTCAGCATTGGCGGCCAGGAAG TTGGTCGCATGAAGATCGAGCTATTTGCAGACGTGGTGCCTAAGACGGCAGAGAACTTTAGGCAATTCTG CACTGGAGAGTTCAGAAAAGATGGCGTTCCAATAGGATACAAAGGAAGCACCTTCCACAGGGTCATAAAG GATTTCATGATTCAGGGTGGAGATTTTGTTAATGGCGATGGCACTGGAGTCGCCAGTATTTACCGGGGTC CATTTGCGGATGAGAATTTTAAACTTAGACACTCTGCTCCAGGCCTGCTTTCCATGGCAAACAGTGGTCC CAGTACAAATGGCTGCCAGTTCTTTATCACGTGTTCTAAGTGTGATTGGCTGGATGGAAAGCATGTAGTG TTTGGAAAAATCATTGACGGACTTCTAGTGATGAGGAAGATCGAGAATGTTCCCACAGGCCCCAACAATA AGCCCAAACTGCCAGTGGTGATCTCACAGTGTGGGGAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201558 protein sequence
Red=Cloning site Green=Tags(s) MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIK DFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVV FGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028677 |
ORF Size | 534 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028677.3, NP_082953.1 |
RefSeq Size | 795 bp |
RefSeq ORF | 534 bp |
Locus ID | 66101 |
UniProt ID | Q9D868 |
Cytogenetics | 4 55.34 cM |
MW | 19.2 kDa |
Gene Summary | PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207560 | Ppih (untagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1, (10ug) |
USD 330.00 |
|
MG201558 | Ppih (tGFP-tagged) - Mouse peptidyl prolyl isomerase H (Ppih) |
USD 500.00 |
|
MR201558L3 | Lenti ORF clone of Ppih (Myc-DDK-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1 |
USD 600.00 |
|
MR201558L4 | Lenti ORF clone of Ppih (mGFP-tagged) - Mouse peptidyl prolyl isomerase H (Ppih), transcript variant 1 |
USD 600.00 |
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