Tomm22 (BC056920) Mouse Tagged ORF Clone
CAT#: MR200911
- TrueORF®
Tomm22 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC056920" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tomm22 |
Synonyms | Tom22, 2310047D01 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR200911 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCCGTCGCTGCAGCCGGCGCTGGGGAGCCTCTGTCCCCCGAGGAATTACTCCCGAAAGCCG AGGCAGAGAAAGCCGAGGAGGAGCTGGAAGAAGACGACGACGACGAGCTAGACGAGACCCTGTCGGAGAG ACTCTGGGGTCTGACGGAGATGTTTCCCGAGAGGGTCCGGTCAGCCGCCGGAGCCACCTTTGACCTCTCG CTCTTCGTGGCTCAGAAGATGTACAGATTTTCCAGGGCAGCTTTGTGGATTGGGACCACTTCCTTCATGA TCCTGGTTCTTCCCGTTGTCTTTGAGACAGAAAAGTTGCAGATGGAGCAACAGCAGCAACTGCAGCAACG GCAGATACTTTTAGGGCCTAACACAGGGCTGTCAGGAGGAATGCCAGGGGCTCTACCTCCACTTCCTGGA AAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200911 protein sequence
Red=Cloning site Green=Tags(s) MAAAVAAAGAGEPLSPEELLPKAEAEKAEEELEEDDDDELDETLSERLWGLTEMFPERVRSAAGATFDLS LFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPPLPG KM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC056920 |
ORF Size | 426 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC056920, AAH56920 |
RefSeq Size | 1029 bp |
RefSeq ORF | 428 bp |
Locus ID | 223696 |
Cytogenetics | 15 E1 |
MW | 15.5 kDa |
Gene Summary | Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG200911 | Tomm22 (tGFP-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) |
USD 350.00 |
|
MR200911L3 | Lenti ORF clone of Tomm22 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) |
USD 450.00 |
|
MR200911L4 | Lenti ORF clone of Tomm22 (mGFP-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) |
USD 450.00 |
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