Ccdc134 (NM_172428) Mouse Tagged ORF Clone

CAT#: MR200630

Ccdc134 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 134 (Ccdc134)

Product Images

Circular map for MR200630

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc134
• Synonyms: 2310042L06Rik; AW208859
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200630 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTTCTTCAGTTCCTGGCAGCCTTCTCTGTCCTCCTGTGGCCTGGCACGGAAGTCACAGGCGCCC
  TGAAGAGCACTCTGGACCCGAGCCTCAAGATTTACAAGAAGATGTTTGAGGTGAAGCGGCGAGAGCAGCT
  GTTGGCACTGAAGAACCTGGCGCAGCTGAATGACATCCACCAGCAGTACAAGATCCTGGATGTCATGCTT
  AAGGGACTCTTCAAGGTGCTGGAGGACTCCCGCACAGTGCTCATTGCTGCCGATGTGCTTCCAGACGGGC
  CGGTCCCCCAGGATGAGAAGCTGAAGGATGCTTTCTCGCACGTGGTGGAGAACACAGCCTTTTTTGGTGA
  CGTCGTGCTGCGCTTCCCAAAGATTGTCCACCACTACTTTGACCATAATTCCAACTGGAACCTCCTGATC
  CGCTGGGGCATCAGCTTCTGCAACCAGACAGGCGTCTTTGACCAGGGGCCCCACTCACCCATCCTCAGCC
  TGATGGCCCAGGAGCTGGGGATCACTGAGAAAGATTCTGACTTCCGGAACCCGTTTAAAACAGACCAGAC
  AGAGTTTATTCCTAGCACCGACCCTTTCCAGAAGGCTTTGAGGGAAGAAGAAAAACGCCGCAAGAAGGAG
  GAGAGGCGGAAGGAGATCCGGAAAGGCCCGAGGATTTCAAGATCCCAGTCTGAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200630 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLLQFLAAFSVLLWPGTEVTGALKSTLDPSLKIYKKMFEVKRREQLLALKNLAQLNDIHQQYKILDVML
  KGLFKVLEDSRTVLIAADVLPDGPVPQDEKLKDAFSHVVENTAFFGDVVLRFPKIVHHYFDHNSNWNLLI
  RWGISFCNQTGVFDQGPHSPILSLMAQELGITEKDSDFRNPFKTDQTEFIPSTDPFQKALREEEKRRKKE
  ERRKEIRKGPRISRSQSEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172428
• ORF Size: 690 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172428.2, NP_766016.2
• RefSeq Size: 2073 bp
• RefSeq ORF: 690 bp
• Locus ID: 76457
• UniProt ID: Q8C7V8
• Cytogenetics: 15 E1
• MW: 26.5 kDa
• Gene Summary: In extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function. Enhances cytotoxic anti-tumor activity of CD8(+) T cells. May inhibit ERK and JNK signaling activity. May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling.[UniProtKB/Swiss-Prot Function]