Pitx2 (NM_011098) Mouse Tagged ORF Clone
CAT#: MG227600
- TrueORF®
Pitx2 (tGFP-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_011098" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pitx2 |
Synonyms | 9430085M16Rik; Brx1; Brx1b; Munc30; Otlx2; Ptx2; Rieg |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227600 representing NM_011098
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAATTGTCGCAAACTAGTGTCGGCCTGCGTGCAATTAGGCGTGCAGCCGGCAGCCGTTGAAT GTCTCTTCTCCAAAGACTCCGAAATCAAAAAGGTCGAGTTCACGGACTCTCCCAAGAGCCGGAAAGAGTC GGCCAGCAGCAAGCTGTTCCCGCGGCAGCACCCCGGCGCCAATGAGAAAGATAAGGGCCAGCAAGGAAAG AATGAGGATGTGGGCGCCGAGGACCCGTCCAAGAAGAAGCGGCAACGCCGGCAGAGGACTCATTTCACTA GCCAGCAGCTGCAGGAGCTGGAAGCCACTTTCCAGAGAAACCGCTACCCAGACATGTCCACTCGCGAAGA AATCGCCGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGCCGGGCCAAATGG AGAAAGCGGGAACGCAACCAGCAGGCCGAGCTGTGCAAGAATGGCTTTGGGCCGCAGTTCAACGGGCTCA TGCAGCCCTACGATGACATGTACCCCGGCTATTCGTACAACAATTGGGCTGCCAAGGGCCTCACGTCAGC GTCTCTGTCCACCAAGAGCTTCCCCTTCTTCAACTCCATGAACGTCAATCCCCTGTCCTCTCAGAGTATG TTTTCCCCGCCCAACTCCATCTCATCTATGAGTATGTCGTCCAGCATGGTGCCCTCCGCGGTGACCGGCG TCCCGGGCTCCAGCCTCAATAGCCTGAATAACTTGAACAACCTGAGCAGCCCGTCGCTGAATTCCGCGGT GCCCACGCCCGCCTGTCCTTACGCGCCGCCGACTCCTCCGTACGTTTATAGGGACACATGTAACTCGAGC CTGGCCAGCCTGAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGCCT CCAACCTGAGTGCTTGCCAGTATGCAGTCGACCGGCCGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227600 representing NM_011098
Red=Cloning site Green=Tags(s) METNCRKLVSACVQLGVQPAAVECLFSKDSEIKKVEFTDSPKSRKESASSKLFPRQHPGANEKDKGQQGK NEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKW RKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSM FSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNSS LASLRLKAKQHSSFGYASVQNPASNLSACQYAVDRPV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011098 |
ORF Size | 951 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011098.4, NP_035228.2 |
RefSeq Size | 1903 bp |
RefSeq ORF | 954 bp |
Locus ID | 18741 |
UniProt ID | P97474 |
Cytogenetics | 3 57.84 cM |
Gene Summary | Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform Ptx2c is involved in left-right asymmetry the developing embryo.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209169 | Pitx2 (untagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 2, (10ug) |
USD 330.00 |
|
MR227600 | Pitx2 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 2 |
USD 330.00 |
|
MR227600L3 | Lenti ORF clone of Pitx2 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 2 |
USD 630.00 |
|
MR227600L4 | Lenti ORF clone of Pitx2 (mGFP-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 2 |
USD 630.00 |
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