Ctla4 (NM_009843) Mouse Tagged ORF Clone
CAT#: MG226896
- TrueORF®
Ctla4 (tGFP-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_009843" in other vectors (6)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ctla4 |
Synonyms | Cd152; Ctla; Ctla-4; Ly-5; Ly-56 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226896 representing NM_009843
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGTCTTGGACTCCGGAGGTACAAAGCTCAACTGCAGCTGCCTTCTAGGACTTGGCCTTTTGTAG CCCTGCTCACTCTTCTTTTCATCCCAGTCTTCTCTGAAGCCATACAGGTGACCCAACCTTCAGTGGTGTT GGCTAGCAGCCATGGTGTCGCCAGCTTTCCATGTGAATATTCACCGTCACACAACACTGATGAGGTCCGG GTGACTGTGCTGCGGCAGACAAATGACCAAATGACTGAGGTCTGTGCCACGACATTCACAGAGAAGAATA CAGTGGGCTTCCTAGATTACCCCTTCTGCAGTGGTACCTTTAATGAAAGCAGAGTGAACCTCACCATCCA AGGACTGAGAGCTGTTGACACGGGACTGTACCTCTGCAAGGTGGAACTCATGTACCCACCGCCATACTTT GTGGGCATGGGCAACGGGACGCAGATTTATGTCATTGATCCAGAACCATGCCCGGATTCTGACTTCCTCC TTTGGATCCTTGTCGCAGTTAGCTTGGGGTTGTTTTTTTACAGTTTCCTGGTCACTGCTGTTTCTTTGAG CAAGATGCTAAAGAAAAGAAGTCCTCTTACAACAGGGGTCTATGTGAAAATGCCCCCAACAGAGCCAGAA TGTGAAAAGCAATTTCAGCCTTATTTTATTCCCATCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226896 representing NM_009843
Red=Cloning site Green=Tags(s) MACLGLRRYKAQLQLPSRTWPFVALLTLLFIPVFSEAIQVTQPSVVLASSHGVASFPCEYSPSHNTDEVR VTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYF VGMGNGTQIYVIDPEPCPDSDFLLWILVAVSLGLFFYSFLVTAVSLSKMLKKRSPLTTGVYVKMPPTEPE CEKQFQPYFIPIN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_009843 |
ORF Size | 669 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009843.2 |
RefSeq ORF | 672 bp |
Locus ID | 12477 |
UniProt ID | P09793 |
Cytogenetics | 1 30.58 cM |
Gene Summary | This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208240 | Ctla4 (untagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4), (10ug) |
USD 300.00 |
|
MR226896 | Ctla4 (Myc-DDK-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) |
USD 450.00 |
|
MR226896L1 | Lenti ORF clone of Ctla4 (Myc-DDK-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) |
USD 750.00 |
|
MR226896L2 | Lenti ORF clone of Ctla4 (mGFP-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) |
USD 750.00 |
|
MR226896L3 | Lenti ORF clone of Ctla4 (Myc-DDK-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) |
USD 750.00 |
|
MR226896L4 | Lenti ORF clone of Ctla4 (mGFP-tagged) - Mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) |
USD 750.00 |
{0} Product Review(s)
Be the first one to submit a review