Cry2 (NM_001113333) Mouse Tagged ORF Clone
CAT#: MG226870
- TrueORF®
Cry2 (tGFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001113333" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Cry2 |
Synonyms | AV006279; D130054K12Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226870 representing NM_001113333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAATCGATGGGCGCGGACGGCGCGTCCTCGG TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGCTAGCTGCCGTGCGCGGGGCGCG CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCTGTGGGCATCAACCGATGG AGGTTCCTACTGCAATCTCTGGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCGCGTCTGTTTGTAG TCCGGGGACAGCCAGCTGATGTGTTCCCAAGGCTGTTCAAGGAATGGGGGGTGACCCGTTTGACCTTTGA ATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCTGGCGTG GAGGTGGTGACTGAGAACTCTCACACCCTCTATGACCTAGACAGAATCATCGAACTGAATGGGCAGAAAC CACCCCTTACCTACAAGCGCTTTCAGGCCCTCATCAGCCGCATGGAGCTGCCCAAGAAGCCCGCGGTGGC TGTGAGCAGCCAGCAGATGGAGAGCTGCAGAGCTGAGATCCAGGAGAACCATGACGACACCTATGGCGTG CCTTCCCTGGAGGAGCTGGGATTCCCCACGGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG AAGCTCTGGCCCGCCTGGACAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT GAATGCCAATTCCTTACTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGATGCCTCTCCTGC CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAAGAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT TATTTGGACAACTCCTGTGGCGAGAATTCTTCTACACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGGCCGAG GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG GGTATTTGACGAGCTGCTCCTGGATGCCGATTTCAGTGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC AGTGCTTTCTTCCAACAATTCTTCCACTGCTACTGCCCTGTGGGCTTCGGCCGACGTACAGACCCCAGTG GGGACTACATCCGGCGGTACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATACATCTATGAGCCCTGGAA TGCCCCCGAGTCAGTTCAGAAGGCTGCCAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCGTCAAT CATGCAGAGACTAGTCGGCTCAACATTGAACGAATGAAGCAGATCTACCAACAGCTGTCGAGATACCGGG GACTCTGTCTATTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC AAGCCAAGCTGGGAGCATCAGCAACACAGGCCCCAGAGCACTATCCAGTGGCCCAGCTTCCCCCAAACGC AAGCTGGAAGCAGCCGAGGAACCTCCTGGTGAAGAACTGACCAAGCGGGCTAGAGTGACGGAGATGCCTA CCCAAGAGCCAGCAAGCAAGGACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226870 representing NM_001113333
Red=Cloning site Green=Tags(s) MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRW RFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGV EVVTENSHTLYDLDRIIELNGQKPPLTYKRFQALISRMELPKKPAVAVSSQQMESCRAEIQENHDDTYGV PSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSC RLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAE GKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSC SAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVN HAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRALSSGPASPKR KLEAAEEPPGEELTKRARVTEMPTQEPASKDS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001113333 |
ORF Size | 1776 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001113333.1, NP_001106804.1 |
RefSeq Size | 2193 bp |
RefSeq ORF | 1779 bp |
Locus ID | 12953 |
Cytogenetics | 2 E1 |
Gene Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219379 | Cry2 (untagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2), transcript variant 2, (10ug) |
USD 607.00 |
|
MR226870 | Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2), transcript variant 2 |
USD 552.00 |
|
MR226870L3 | Lenti ORF clone of Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2), transcript variant 2 |
USD 852.00 |
|
MR226870L4 | Lenti ORF clone of Cry2 (mGFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2), transcript variant 2 |
USD 852.00 |
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