Tlr7 (NM_133211) Mouse Tagged ORF Clone

CAT#: MG226835

Tlr7 (tGFP-tagged) - Mouse toll-like receptor 7 (Tlr7), (10ug)

Product Images

Circular map for MG226835

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tlr7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226835 representing NM_133211
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTTCGATGTGGACACGGAAGAGACAAATTTTGATCTTTTTAAATATGCTCTTAGTTTCTAGAG
  TCTTTGGGTTTCGATGGTTTCCTAAAACTCTACCTTGTGAAGTTAAAGTAAATATCCCAGAGGCCCATGT
  GATCGTGGACTGCACAGACAAGCATTTGACAGAAATCCCTGAGGGCATTCCCACTAACACCACCAATCTT
  ACCCTTACCATCAACCACATACCAAGCATCTCTCCAGATTCCTTCCGTAGGCTGAACCATCTGGAAGAAA
  TCGATTTAAGATGCAATTGTGTACCTGTTCTACTGGGGTCCAAAGCCAATGTGTGTACCAAGAGGCTGCA
  GATTAGACCTGGAAGCTTTAGTGGACTCTCTGACTTAAAAGCCCTTTACCTGGATGGAAACCAACTTCTG
  GAGATACCACAGGATCTGCCATCCAGCTTACATCTTCTGAGCCTTGAGGCTAACAACATCTTCTCCATCA
  CGAAGGAGAATCTAACAGAACTGGTCAACATTGAAACACTCTACCTGGGTCAAAACTGTTATTATCGAAA
  TCCTTGCAATGTTTCCTATTCTATTGAAAAAGATGCTTTCCTAGTTATGAGAAATTTGAAGGTTCTCTCA
  CTAAAAGATAACAATGTCACAGCTGTCCCCACCACTTTGCCACCTAATTTACTAGAGCTCTATCTTTATA
  ACAATATCATTAAGAAAATCCAAGAAAATGATTTTAATAACCTCAATGAGTTGCAAGTTCTTGACCTAAG
  TGGAAATTGCCCTCGATGTTATAATGTCCCATATCCGTGTACACCGTGTGAAAATAATTCCCCCTTACAG
  ATCCATGACAATGCTTTCAATTCATTGACAGAATTAAAAGTTTTACGTTTACACAGTAATTCTCTTCAGC
  ATGTGCCCCCAACATGGTTTAAAAACATGAGAAACCTCCAGGAACTAGACCTCTCCCAAAACTACTTGGC
  CAGAGAAATTGAGGAGGCCAAATTTTTGCATTTTCTTCCCAACCTTGTTGAGTTGGATTTTTCTTTCAAT
  TATGAGCTGCAGGTCTACCATGCATCTATAACTTTACCACATTCACTCTCTTCATTGGAAAACTTGAAAA
  TTCTGCGTGTCAAGGGGTATGTCTTTAAAGAGCTGAAAAACTCCAGTCTTTCTGTATTGCACAAGCTTCC
  CAGGCTGGAAGTTCTTGACCTTGGCACTAACTTCATAAAAATTGCTGACCTCAACATATTCAAACATTTT
  GAAAACCTCAAACTCATAGACCTTTCAGTGAATAAGATATCTCCTTCAGAAGAGTCAAGAGAAGTTGGCT
  TTTGTCCTAATGCTCAAACTTCTGTAGACCGTCATGGGCCCCAGGTCCTTGAGGCCTTACACTATTTCCG
  ATACGATGAATATGCACGGAGCTGCAGGTTCAAAAACAAAGAGCCACCTTCTTTCTTGCCTTTGAATGCA
  GACTGCCACATATATGGGCAGACCTTAGACTTAAGTAGAAATAACATATTTTTTATTAAACCTTCTGATT
  TTCAGCATCTTTCATTCCTCAAATGCCTCAACTTATCAGGAAACACCATTGGCCAAACTCTTAATGGCAG
  TGAACTCTGGCCGTTGAGAGAGTTGCGGTACTTAGACTTCTCCAACAACCGGCTTGATTTACTCTACTCA
  ACAGCCTTTGAAGAGCTCCAGAGTCTTGAAGTTCTGGATCTAAGTAGTAACAGCCACTATTTTCAAGCAG
  AAGGAATTACTCACATGCTAAACTTTACCAAGAAATTACGGCTTCTGGACAAACTCATGATGAATGATAA
  TGACATCTCTACTTCGGCCAGCAGGACCATGGAAAGTGACTCTCTTCGAATTCTGGAGTTCAGAGGCAAC
  CATTTAGATGTTCTATGGAGAGCCGGTGATAACAGATACTTGGACTTCTTCAAGAATTTGTTCAATTTAG
  AGGTATTAGATATCTCCAGAAATTCCCTGAATTCCTTGCCTCCTGAGGTTTTTGAGGGTATGCCGCCAAA
  TCTAAAGAATCTCTCCTTGGCCAAAAATGGGCTCAAATCTTTCTTTTGGGACAGACTCCAGTTACTGAAG
  CATTTGGAAATTTTGGACCTCAGCCATAACCAGCTGACAAAAGTACCTGAGAGATTGGCCAACTGTTCCA
  AAAGTCTCACAACACTGATTCTTAAGCATAATCAAATCAGGCAATTGACAAAATATTTTCTAGAAGATGC
  TTTGCAATTGCGCTATCTAGACATCAGTTCAAATAAAATCCAGGTCATTCAGAAGACTAGCTTCCCAGAA
  AATGTCCTCAACAATCTGGAGATGTTGGTTTTACATCACAATCGCTTTCTTTGCAACTGTGATGCTGTGT
  GGTTTGTCTGGTGGGTTAACCATACAGATGTTACTATTCCATACCTGGCCACTGATGTGACTTGTGTAGG
  TCCAGGAGCACACAAAGGTCAAAGTGTCATATCCCTTGATCTGTATACGTGTGAGTTAGATCTCACAAAC
  CTGATTCTGTTCTCAGTTTCCATATCATCAGTCCTCTTTCTTATGGTAGTTATGACAACAAGTCACCTCT
  TTTTCTGGGATATGTGGTACATTTATTATTTTTGGAAAGCAAAGATAAAGGGGTATCAGCATCTGCAATC
  CATGGAGTCTTGTTATGATGCTTTTATTGTGTATGACACTAAAAACTCAGCTGTGACAGAATGGGTTTTG
  CAGGAGCTGGTGGCAAAATTGGAAGATCCAAGAGAAAAACACTTCAATTTGTGTCTAGAAGAAAGAGACT
  GGCTACCAGGACAGCCAGTTCTAGAAAACCTTTCCCAGAGCATACAGCTCAGCAAAAAGACAGTGTTTGT
  GATGACACAGAAATATGCTAAGACTGAGAGTTTTAAGATGGCATTTTATTTGTCTCATCAGAGGCTCCTG
  GATGAAAAAGTGGATGTGATTATCTTGATATTCTTGGAAAAGCCTCTTCAGAAGTCTAAGTTTCTTCAGC
  TCAGGAAGAGACTCTGCAGGAGCTCTGTCCTTGAGTGGCCTGCAAATCCACAGGCTCACCCATACTTCTG
  GCAGTGCCTGAAAAATGCCCTGACCACAGACAATCATGTGGCTTATAGTCAAATGTTCAAGGAAACAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226835 representing NM_133211
  Red=Cloning site Green=Tags(s)
  
  MVFSMWTRKRQILIFLNMLLVSRVFGFRWFPKTLPCEVKVNIPEAHVIVDCTDKHLTEIPEGIPTNTTNL
  TLTINHIPSISPDSFRRLNHLEEIDLRCNCVPVLLGSKANVCTKRLQIRPGSFSGLSDLKALYLDGNQLL
  EIPQDLPSSLHLLSLEANNIFSITKENLTELVNIETLYLGQNCYYRNPCNVSYSIEKDAFLVMRNLKVLS
  LKDNNVTAVPTTLPPNLLELYLYNNIIKKIQENDFNNLNELQVLDLSGNCPRCYNVPYPCTPCENNSPLQ
  IHDNAFNSLTELKVLRLHSNSLQHVPPTWFKNMRNLQELDLSQNYLAREIEEAKFLHFLPNLVELDFSFN
  YELQVYHASITLPHSLSSLENLKILRVKGYVFKELKNSSLSVLHKLPRLEVLDLGTNFIKIADLNIFKHF
  ENLKLIDLSVNKISPSEESREVGFCPNAQTSVDRHGPQVLEALHYFRYDEYARSCRFKNKEPPSFLPLNA
  DCHIYGQTLDLSRNNIFFIKPSDFQHLSFLKCLNLSGNTIGQTLNGSELWPLRELRYLDFSNNRLDLLYS
  TAFEELQSLEVLDLSSNSHYFQAEGITHMLNFTKKLRLLDKLMMNDNDISTSASRTMESDSLRILEFRGN
  HLDVLWRAGDNRYLDFFKNLFNLEVLDISRNSLNSLPPEVFEGMPPNLKNLSLAKNGLKSFFWDRLQLLK
  HLEILDLSHNQLTKVPERLANCSKSLTTLILKHNQIRQLTKYFLEDALQLRYLDISSNKIQVIQKTSFPE
  NVLNNLEMLVLHHNRFLCNCDAVWFVWWVNHTDVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTN
  LILFSVSISSVLFLMVVMTTSHLFFWDMWYIYYFWKAKIKGYQHLQSMESCYDAFIVYDTKNSAVTEWVL
  QELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTQKYAKTESFKMAFYLSHQRLL
  DEKVDVIILIFLEKPLQKSKFLQLRKRLCRSSVLEWPANPQAHPYFWQCLKNALTTDNHVAYSQMFKETV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133211
• ORF Size: 3150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133211.4, NP_573474.1
• RefSeq Size: 3746 bp
• RefSeq ORF: 3153 bp
• Locus ID: 170743
• UniProt ID: P58681
• Cytogenetics: X F5
• Gene Summary: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.[UniProtKB/Swiss-Prot Function]