Ptk2b (NM_172498) Mouse Tagged ORF Clone
CAT#: MG226795
- TrueORF®
Ptk2b (tGFP-tagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b) transcript variant 3, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_172498" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ptk2b |
Synonyms | CADTK; CAKB; CAKbeta; E430023O05Rik; FADK2; FAK2; PYK2; Raftk |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226795 representing NM_172498
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGGGTGTCTGAGCCCTTGAGCCGTGTAAAAGTGGGCACTTTACGCCGGCCTGAGGGCCCCCCAG AGCCCATGGTGGTGGTACCAGTGGATGTGGAGAAGGAAGACGTGCGCATCCTCAAGGTCTGCTTCTACAG CAACAGCTTCAACCCAGGGAAGAACTTCAAGCTTGTCAAATGCACAGTGCAGACAGAGATCCAGGAGATC ATCACCTCCATCCTCCTGAGTGGGCGAATAGGGCCCAACATCCAGCTGGCTGAATGCTATGGGCTGAGGC TGAAGCACATGAAGTCAGACGAGATCCACTGGCTGCACCCACAGATGACCGTGGGCGAAGTGCAGGACAA GTATGAATGTCTACACGTGGAAGCTGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCGGAAGACTTC ATGGAGAGCCTGAAAGAAGACAGGACCACATTGCTGTACTTTTATCAACAGCTCCGGAATGACTACATGC AACGCTACGCCAGCAAGGTCAGTGAAGGCATGGCTCTGCAGCTGGGCTGTCTGGAGCTCAGGAGATTCTT CAAGGACATGCCCCACAATGCACTGGACAAAAAGTCCAACTTTGAACTCCTGGAAAAAGAAGTCGGTCTG GACCTGTTTTTCCCAAAGCAGATGCAGGAAAACTTAAAGCCCAAGCAGTTCCGGAAGATGATCCAGCAGA CCTTCCAGCAGTATGCATCACTCCGGGAGGAAGAGTGTGTCATGAAATTCTTCAATACCCTAGCGGGCTT TGCCAACATTGACCAGGAGACCTACCGCTGCGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC ATCGGCCCTAAAGGCATCCGTCAGCTGACAAGTCAAGATACAAAGCCCACCTGCCTGGCCGAGTTTAAGC AGATCAAATCCATCAGGTGCCTCCCATTGGAAGAGACCCAGGCAGTCCTGCAGCTGGGCATCGAGGGTGC CCCCCAGTCCTTGTCTATCAAAACGTCGTCCCTGGCAGAGGCTGAGAACATGGCTGACCTCATAGATGGC TACTGCAGGCTGCAAGGAGAACATAAGGGCTCTCTCATCATGCATGCCAAGAAAGATGGTGAGAAGAGGA ACAGCCTGCCTCAGATCCCCACACTAAACCTGGAGGCTCGGCGGTCGCACCTCTCAGAAAGCTGCAGCAT AGAGTCAGACATCTATGCGGAGATTCCCGATGAGACCCTGCGAAGACCAGGAGGTCCACAGTACGGTGTT GCCCGTGAAGAAGTAGTTCTTAACCGCATTCTGGGTGAAGGCTTCTTTGGGGAGGTCTATGAAGGTGTCT ACACGAACCACAAAGGGGAAAAAATTAATGTGGCCGTCAAGACCTGTAAGAAAGACTGTACCCAGGACAA CAAGGAGAAGTTCATGAGTGAGGCAGTGATCATGAAGAATCTTGACCACCCTCACATCGTGAAGCTGATT GGCATCATTGAAGAGGAACCCACCTGGATTATCATGGAACTGTATCCTTATGGGGAGCTGGGACACTACC TGGAACGAAATAAAAACTCCCTGAAGGTACCCACTCTGGTCCTGTACACCCTACAGATATGCAAAGCCAT GGCCTATCTGGAGAGCATCAACTGTGTGCACAGGGATATTGCTGTCCGGAACATCCTGGTGGCCTCTCCT GAGTGTGTGAAGCTGGGGGACTTTGGGCTCTCCCGGTACATTGAGGACGAAGACTATTACAAAGCCTCTG TGACCCGTCTACCCATCAAATGGATGTCCCCCGAGTCCATCAACTTCCGCCGCTTCACAACCGCCAGTGA TGTCTGGATGTTTGCTGTATGCATGTGGGAGATCCTCAGCTTTGGGAAGCAGCCTTTCTTCTGGCTCGAA AATAAGGATGTCATCGGAGTGCTGGAGAAAGGGGACAGGCTGCCCAAGCCCGAACTCTGTCCGCCTGTCC TTTACACACTCATGACTCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACGGAGCTTGTGTG CAGCCTCAGTGACATTTATCAGATGGAGAAGGACATTGCCATAGAGCAAGAAAGGAATGCTCGCTACCGA CCCCCCAAAATATTGGAGCCTACTACCTTTCAGGAACCCCCACCCAAGCCCAGCCGGCCCAAGTACAGAC CTCCTCCACAGACCAACCTGCTGGCTCCTAAGCTGCAGTTCCAGGAGGAGGACTTCATCCGGCCCAGTAG CCGAGAAGAGGCCCAGCAGCTCTGGGAGGCAGAGAAGATCAAGATGAAGCAGGTCCTAGAAAGACAGCAG AAGCAGATGGTGGAAGATTCCCAGTGGCTGAGGCGAGAGGAAAGATGCTTGGACCCTATGGTTTATATGA ATGACAAGTCCCCACTGACTCCAGAGAAGGAGGCCGGCTACACGGAGTTCACAGGGCCCCCACAGAAACC ACCTCGGCTCGGTGCACAGTCCATTCAGCCCACAGCCAACCTGGACAGGACCGATGACCTCGTGTACCAC AATGTCATGACCCTGGTGGAGGCTGTGCTGGAACTCAAGAACAAGCTTGGCCAGTTGCCCCCTGAGGACT ATGTGGTGGTGGTGAAGAACGTGGGGCTGAACCTGCGGAAGCTCATCGGCAGTGTGGACGATCTCTTGCC CTCCTTGCCGGCATCTTCGAGGACAGAGATTGAAGGGACCCAGAAACTGCTCAACAAAGACCTGGCAGAG CTCATCAACAAGATGAAGTTGGCTCAGCAGAACGCCGTGACGTCCCTGAGTGAGGACTGCAAGCGGCAGA TGCTCACAGCGTCCCATACCCTGGCTGTGGATGCCAAGAACCTGCTGGATGCTGTGGACCAAGCCAAGGT TGTGGCTAATCTGGCCCACCCGCCTGCAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226795 representing NM_172498
Red=Cloning site Green=Tags(s) MSGVSEPLSRVKVGTLRRPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEI ITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDF MESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGL DLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLV IGPKGIRQLTSQDTKPTCLAEFKQIKSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDG YCRLQGEHKGSLIMHAKKDGEKRNSLPQIPTLNLEARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGV AREEVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTQDNKEKFMSEAVIMKNLDHPHIVKLI GIIEEEPTWIIMELYPYGELGHYLERNKNSLKVPTLVLYTLQICKAMAYLESINCVHRDIAVRNILVASP ECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLE NKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMEKDIAIEQERNARYR PPKILEPTTFQEPPPKPSRPKYRPPPQTNLLAPKLQFQEEDFIRPSSREEAQQLWEAEKIKMKQVLERQQ KQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYH NVMTLVEAVLELKNKLGQLPPEDYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAE LINKMKLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172498 |
ORF Size | 2901 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172498.3, NP_766086.2 |
RefSeq Size | 3925 bp |
RefSeq ORF | 2904 bp |
Locus ID | 19229 |
Cytogenetics | 14 34.36 cM |
Gene Summary | Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376' (By similarity). Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222867 | Ptk2b (untagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b), transcript variant 3, (10ug) |
USD 975.00 |
|
MR226795 | Ptk2b (Myc-DDK-tagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b), transcript variant 3 |
USD 974.00 |
|
MR226795L3 | Lenti ORF clone of Ptk2b (Myc-DDK-tagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b), transcript variant 3 |
USD 1,274.00 |
|
MR226795L4 | Lenti ORF clone of Ptk2b (mGFP-tagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b), transcript variant 3 |
USD 1,274.00 |
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