Chrna3 (NM_145129) Mouse Tagged ORF Clone
CAT#: MG226689
- TrueORF®
Chrna3 (tGFP-tagged) - Mouse cholinergic receptor nicotinic alpha polypeptide 3 (Chrna3), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_145129" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Chrna3 |
Synonyms | (a)3; A730007P14Rik; Acra-3; Acra3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226689 representing NM_145129
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTCTTCAGACATGGGTGTTGTGCTGCCCCCGCCGCCGCTGTCCATGCTGATGCTGGTGCTGATGC TGCTGCCAGTGGCCAGCGCCTCGGAAGCTGAGCACCGCCTGTTCCAGTACCTGTTTGAAGATTACAACGA GATCATCCGGCCGGTGGCTAACGTGTCCCATCCTGTCATCATCCAGTTTGAGGTGTCTATGTCTCAGCTG GTGAAGGTGGATGAAGTAAACCAGATCATGGAAACCAACCTGTGGCTGAAGCAAATCTGGAATGACTACA AGCTGAAATGGAAACCCTCTGACTACCAAGGGGTGGAGTTCATGCGAGTCCCTGCAGAGAAGATCTGGAA ACCAGACATCGTGCTTTACAACAACGCCGATGGGGATTTCCAAGTGGATGACAAAACCAAAGCTCTACTC AAGTACACAGGAGAAGTGACTTGGATCCCTCCGGCCATCTTTAAGAGCTCATGCAAAATCGATGTGACCT ACTTCCCGTTTGACTACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGA CCTGGTCCTCATTGGCTCCTCCATGAACCTCAAGGACTATTGGGAAAGTGGCGAGTGGGCCATCATTAAA GCCCCGGGCTACAAACATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGC TATACATTCGCCGCCTGCCGCTGTTCTACACCATCAACCTCATCATTCCGTGCCTGCTCATCTCCTTCCT CACTGTGCTCGTCTTCTACCTGCCCTCCGACTGTGGGGAGAAGGTGACGCTCTGCATCTCCGTGCTCCTC TCCCTGACGGTCTTTCTCCTCGTGATCACCGAGACCATCCCTTCCACCTCACTGGTCATCCCCTTGATCG GGGAGTACCTCCTCTTCACTATGATTTTTGTCACCTTGTCCATCGTCATCACAGTCTTTGTGCTCAACGT GCACTACAGAACTCCGACCACACACACGATGCCCACTTGGGTCAAGGCTGTGTTTTTGAACCTTCTCCCC AGGGTCATGTTTATGACTAGGCCAACCAGCACCGAGGAAGACGCCCCAAAGACGAGGAACTTCTACGGTG CTGAGCTCTCAAACCTGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCA AGATGGGACATGTGGCTATTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGA AGCTCCAGTTCTGAGTCTGTTGATGCTGTGTTGTCCCTGTCTGCTCTGTCACCAGAAATCAAAGAAGCCA TCCAAAGTGTGAAGTATATTGCTGAAAATATGAAAGCACAGAATGTAGCCAAAGAGATTCAAGATGATTG GAAGTATGTTGCCATGGTGATTGATCGCATTTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACT GCAGGATTATTTCTGCAACCCTTGATGGCCAGAGATGACACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226689 representing NM_145129
Red=Cloning site Green=Tags(s) MRSSDMGVVLPPPPLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQL VKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALL KYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIK APGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLL SLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLP RVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTR SSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGT AGLFLQPLMARDDT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145129 |
ORF Size | 1512 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145129.2, NP_660111.2 |
RefSeq Size | 3123 bp |
RefSeq ORF | 1515 bp |
Locus ID | 110834 |
UniProt ID | Q8R4G9 |
Cytogenetics | 9 B |
Gene Summary | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217192 | Chrna3 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), (10ug) |
USD 470.00 |
|
MR226689 | Chrna3 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3) |
USD 470.00 |
|
MR226689L3 | Lenti ORF clone of Chrna3 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3) |
USD 770.00 |
|
MR226689L4 | Lenti ORF clone of Chrna3 (mGFP-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3) |
USD 770.00 |
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