Nkx6-3 (NM_029002) Mouse Tagged ORF Clone

CAT#: MG226363

Nkx6 (tGFP-tagged) - Mouse NK6 homeobox 3 (Nkx6-3), (10ug)

Product Images

Circular map for MG226363

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nkx6-3
• Synonyms: 9130417I07Rik; Nkx6.3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226363 representing NM_029002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCAACCTGCAGGGGACCTTCCTCCTGAACAACACACAGCTGGCCCAGTTCTCAGAAATGAAGG
  CTCCCATGTGCCAGTACTCGGTGCAGAACTCTTTCTACAAGCTCAGCCCTCCGGGACTTGGCCCCCAGCT
  GGCGGCTGGGACTCCCCATGGAATCACTGACATCCTGAGCAGACCGGTTGCCACCCCGAACAGCAGCCTC
  CTCTCTGGCTACCCCCACGTGGCAGGCTTTGGTGGACTCAGCTCCCAGGGCGTCTACTATGGCCCCCAGG
  TGGGGAGCTTTTCCAAGGCTGGAAATGAGTACCCGACCCGGACCCGGAACTGCTGGGCAGACACAGGCCA
  GGACTGGAGAGGCAGCGCTCGGCCATGCAGCAACACTCCAGACCCCCTGAGTGACACTATCCACAAGAAA
  AAGCACACCCGCCCTACCTTCACGGGCCACCAGATCTTTGCCCTGGAGAAAACCTTTGAGCAGACCAAGT
  ACTTGGCTGGCCCAGAGCGGGCGAGGCTGGCTTATTCCCTGGGCATGACGGAGTCACAGGTCAAGGTGTG
  GTTCCAGAACCGCCGGACCAAGTGGAGGAAGAAGAGCGCCCTGGAGCCCTCATCCTCCACACCCCGGGCC
  CCGGGAGGTGCGAGCGGGGACCGCGCGGCCTCGGAGAACGAGGACGATGAGTACAACAAGCCGCTGGACC
  CGGACTCGGACGACGAGAAGATCCGCCTGCTGCTGCGCAAGCACCGGGCCGCCTTCTCCGTGCTCAGCCT
  GGGCGCGCACAGCGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226363 representing NM_029002
  Red=Cloning site Green=Tags(s)
  
  MESNLQGTFLLNNTQLAQFSEMKAPMCQYSVQNSFYKLSPPGLGPQLAAGTPHGITDILSRPVATPNSSL
  LSGYPHVAGFGGLSSQGVYYGPQVGSFSKAGNEYPTRTRNCWADTGQDWRGSARPCSNTPDPLSDTIHKK
  KHTRPTFTGHQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKKSALEPSSSTPRA
  PGGASGDRAASENEDDEYNKPLDPDSDDEKIRLLLRKHRAAFSVLSLGAHSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029002
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029002.2, NP_083278.1
• RefSeq Size: 2236 bp
• RefSeq ORF: 789 bp
• Locus ID: 74561
• UniProt ID: Q3UHX8
• Cytogenetics: 8 A2
• Gene Summary: Putative transcription factor, which may be involved in patterning of central nervous system and pancreas.[UniProtKB/Swiss-Prot Function]