Musk (NM_010944) Mouse Tagged ORF Clone
CAT#: MG226300
- TrueORF®
Musk (tGFP-tagged) - Mouse muscle skeletal receptor tyrosine kinase (Musk) transcript variant 4, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010944" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Musk |
Synonyms | Mdk4; Mlk; Ns; Nsk1; Nsk2; Nsk3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226300 representing NM_010944
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAGCTTGTCAACATTCCACTGTTACAGATGCTCACCCTGGTTGCCTTCAGCGGGACTGAGAAAC TTCCAAAAGCCCCTGTCATCACCACGCCTCTTGAAACTGTAGATGCCTTGGTTGAAGAAGTAGCGACTTT CATGTGTGCCGTGGAATCCTACCCTCAGCCCGAGATTTCTTGGACCAGAAATAAAATTCTCATTAAGCTG TTTGACACCCGCTACAGCATCCGGGAGAATGGTCAGCTCCTCACCATTCTGAGCGTGGAAGACAGTGATG ATGGCATCTACTGCTGCATAGCCAACAATGGAGTGGGAGGAGCCGTGGAGAGTTGTGGTGCCCTGCAAGT GAAGATGAAACCTAAAATAACTCGTCCTCCCATTAATGTAAAAATAATAGAGGGATTGAAGGCAGTTCTG CCGTGCACTACGATGGGTAACCCCAAACCATCTGTGTCCTGGATCAAGGGGGACAATGCTCTCAGGGAAA ATTCCAGAATCGCAGTTCTTGAATCTGGGAGCTTAAGGATCCATAATGTGCAAAAGGAAGATGCAGGACA GTACCGCTGTGTGGCCAAAAACAGCCTGGGCACAGCTTACTCCAAACTGGTGAAGCTGGAAGTGGAGGTT TTTGCAAGAATCCTGCGTGCTCCTGAATCCCACAATGTCACCTTTGGTTCCTTTGTAACCCTACGCTGCA CAGCAATAGGCATCCCTGTCCCCACCATCAGCTGGATTGAAAACGGAAATGCTGTTTCTTCAGGTTCCAT TCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTCCAGCTCTTCATCACAAAGCCAGGACTCTAC ACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTACCGCAAAGGCTGCAGCCACTGTCAGCATAG CAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGCTACTGTGCCCAGTACAGAGGGGAGGTGTGTGATGC AGTCCTGGCGAAAGATGCTCTTGTCTTCTTCAACACCTCCTACCGGGACCCCGAGGACGCCCAGGAGCTG CTGATCCACACTGCGTGGAATGAGCTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTGAGGCTCTGC TGTGTAACCACCTCTTCCAAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTGCAGAGAGTA CTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCCCACCGGGGC CTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGCAAGCTTCCCAGCATGCACCGGGACC CCACAGCCTGCACAAGACTGCCATATTTAGATTATAAAAAAGAAAACATAACAACATTCCCGTCAATAAC GTCCTCCAGGCCGAGCGCGGACATTCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTCTCGCCTGCG TACTCCATGACCGTCATCATCTCCATCGTGTCCAGCTTTGCCCTGTTTGCTCTTCTCACCATCGCTACTC TCTATTGCTGCCGAAGGAGGAAAGAATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGACCCTCACCAC GTTGCCTTCCGAGCTCCTGCTGGATAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCACTCCTTCTG AATCCTAAGTTGCTCAGCCTGGAGTATCCGAGGAATAACATTGAGTATGTCCGAGACATCGGAGAGGGGG CGTTTGGAAGAGTCTTCCAAGCAAGGGCCCCTGGCTTGCTGCCTTATGAACCTTTCACTATGGTGGCCGT GAAGATGCTTAAGGAAGAGGCCTCTGCAGACATGCAAGCGGACTTTCAGAGGGAGGCGGCCCTCATGGCA GAGTTTGACAACCCCAACATTGTGAAACTCTTAGGTGTGTGTGCCGTTGGGAAGCCGATGTGTCTGCTCT TTGAATATATGGCCTATGGTGACCTCAATGAGTTCCTCCGAAGTATGTCCCCGCACACTGTTTGCAGCCT CAGCCACAGTGACCTGTCCACGAGGGCTCGGGTGTCTAGCCCTGGTCCTCCACCACTGTCCTGTGCAGAA CAGCTCTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCCTACCTTTCAGAGCGCAAGTTTGTCCACCGGG ACTTAGCTACCAGGAACTGCCTGGTTGGGGAGACCATGGTGGTGAAAATTGCAGACTTTGGCCTCTCCAG GAACATCTATTCCGCAGACTACTACAAAGCTGATGGAAATGACGCCATCCCTATCCGCTGGATGCCGCCC GAGTCTATCTTCTACAACCGCTACACCACTGAGTCGGATGTATGGGCCTATGGTGTGGTCCTCTGGGAGA TCTTCTCCTATGGGCTGCAGCCCTACTATGGAATGGCCCACGAGGAGGTCATTTACTATGTGAGAGATGG CAACATCCTCGCCTGCCCTGAGAACTGCCCCTTGGAACTGTACAACCTCATGCGCCTGTGTTGGAGCAAG CTGCCTGCTGATAGACCCAGCTTCTGCAGTATCCACAGGATCCTGCAGCGCATGTGCGAGAGAGCAGAGG GAACGGTGGGTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226300 representing NM_010944
Red=Cloning site Green=Tags(s) MRELVNIPLLQMLTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL FDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL PCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEV FARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLY TCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYRGEVCDAVLAKDALVFFNTSYRDPEDAQEL LIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRG LYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPSITSSRPSADIPNLPASTSSFAVSPA YSMTVIISIVSSFALFALLTIATLYCCRRRKEWKNKKRESTAVTLTTLPSELLLDRLHPNPMYQRMPLLL NPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMA EFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSTRARVSSPGPPPLSCAE QLCIARQVAAGMAYLSERKFVHRDLATRNCLVGETMVVKIADFGLSRNIYSADYYKADGNDAIPIRWMPP ESIFYNRYTTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSK LPADRPSFCSIHRILQRMCERAEGTVGV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010944 |
ORF Size | 2604 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010944.2, NP_035074.2 |
RefSeq Size | 3354 bp |
RefSeq ORF | 2607 bp |
Locus ID | 18198 |
UniProt ID | Q61006 |
Cytogenetics | 4 31.87 cM |
Gene Summary | This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222258 | Musk (untagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 4, (10ug) |
USD 875.00 |
|
MR226300 | Musk (Myc-DDK-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 4 |
USD 874.00 |
|
MR226300L3 | Lenti ORF clone of Musk (Myc-DDK-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 4 |
USD 1,174.00 |
|
MR226300L4 | Lenti ORF clone of Musk (mGFP-tagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 4 |
USD 1,174.00 |
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