Capn3 (NM_007601) Mouse Tagged ORF Clone

CAT#: MG226221

Capn3 (tGFP-tagged) - Mouse calpain 3 (Capn3) transcript variant a, (10ug)

Product Images

Circular map for MG226221

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Capn3
• Synonyms: AI323605; Capa-3; Capa3; Lp82; p94
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226221 representing NM_007601
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAACTGTTATTAGTCCAACTGTGGCCCCAAGGACGGGGGCTGAGCCCAGGTCCCCAGGGCCAGTTC
  CTCACCCAGCTCAAGGCAAGACCACCGAGGCTGGAGGTGGACACCCAAGTGGCATCTATTCGGCCATCAT
  TAGCCGCAATTTTCCGATCATCGGTGTGAAAGAGAAGACATTTGAGCAGCTCCGCAGGAAGTGCCTAGAG
  AAGAAAGTTCTTTATCTAGACCCCGAGTTCCCACCGGATGAGACCTCTCTCTTTTACAGTCAGAAGTTCC
  CCATCCAGTTCGTCTGGAAGAGACCTCCGGAAATTTGTGAGAATCCCCGGTTTATCATTGGTGGAGCCAA
  CAGGACTGACATCTGCCAAGGAGATCTAGGGGACTGCTGGTTTCTTGCAGCCATCGCCTGCCTGACCCTG
  AATGAGCGGCTGCTTTTCCGAGTTATACCCCATGATCAAAGTTTCACTGAAAACTACGCAGGGATCTTCC
  ACTTCCAGTTCTGGCGCTATGGAGACTGGGTAGATGTGGTCATTGACGACTGTCTGCCAACATACAACAA
  TCAGCTGGTTTTCACCAAATCCAACCACCGCAATGAGTTCTGGAGTGCTCTCCTTGAGAAGGCTTATGCC
  AAGCTCCATGGCTCCTATGAAGCTCTGAAAGGTGGGAATACCACAGAAGCCATGGAGGACTTCACAGGAG
  GGGTGACAGAGTTTTTTGAGATCAAGGATGCTCCGAGTGACATGTACAAGATTATGAGGAAAGCTATCGA
  GAGAGGCTCCCTCATGGGCTGCTCCATTGACGATGGCACGAACATGACTTATGGAACCTCTCCTTCTGGT
  CTGAACATGGGGGAGTTGATTGCACGGATGGTGAGAAATATGGATAACTCGCTGCTCAGAGACTCAGACC
  TGGACCCCAGGGGCTCAGATGACAGACCGTCACGGACAATTGTTCCAGTGCAGTATGAAACAAGAATGGC
  CTGTGGGTTGGTGAAAGGGCATGCCTATTCAGTCACTGGGCTGGAGGAGGCCCTGTTCAAAGGTGAGAAG
  GTGAAGTTGGTGCGGCTGCGGAACCCCTGGGGCCAGGTGGAGTGGAACGGCTCTTGGAGTGATGGTTGGA
  AGGACTGGAGCTTTGTAGACAAAGATGAGAAGGCCCGTCTACAGCACCAGGTTACTGAGGATGGAGAGTT
  CTGGATGTCATATGATGACTTCGTCTACCATTTCACGAAGCTGGAGATCTGCAACCTCACAGCTGACGCC
  CTGGAGTCCGATAAGCTTCAGACCTGGACGGTGTCTGTAAACGAGGGCCGCTGGGTGAGGGGCTGTTCTG
  CTGGAGGCTGCCGAAACTTCCCAGACACTTTCTGGACCAACCCGCAGTACCGTCTCAAGCTTCTGGAGGA
  AGACGATGACCCTGAGGACTCTGAGGTAATCTGCAGCTTCCTCGTGGCTCTGATGCAGAAGAACCGGCGC
  AAGGACCGGAAGCTGGGGGCCAACCTCTTCACTATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGC
  ACGGGAATAAGCAACACCTGCAGAAGGACTTCTTCTTGTACAACGCCTCCAAGGCCAGAAGCAAAACCTA
  CATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCACCCAGCGAGTATGTCATCGTACCCTCCACC
  TACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGAGTCTTCTCCGAAAAGAGGAATCTCTCTGAGGAAG
  CTGAAAATACAATCTCTGTGGATCGGCCAGTGAAAAAGAAAAAAAACAAGCCCATCATCTTCGTTTCTGA
  CAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGGCAGAGGAGGGCAAAGACAAAGCAGGGCCC
  GAGAAACGAGGGGAAACCCCACAGCCAAGGCCTGGCCACACAGACCAGGAAAGTGAGGAGCAGCAGCAAT
  TCCGGAACATCTTCAGGCAGATCGCAGGCGACGACATGGAGATCTGTGCAGATGAACTCAAGAATGTCCT
  CAACACAGTGGTGAACAAACACAAGGACCTGAAGACACAAGGGTTCACGCTGGAGTCCTGCAGAAGCATG
  ATAGCTCTCATGGATACAGATGGCTCTGGAAGACTGAATCTTCAAGAGTTCCATCACCTCTGGAAAAAGA
  TCAAGGCCTGGCAGAAAATCTTCAAGCACTATGACACAGACCATTCCGGTACCATCAATAGCTATGAGAT
  GCGAAATGCAGTCAATGATGCAGGCTTCCATCTCAACAGCCAACTCTATGACATCATCACCATGCGCTAT
  GCGGACAAACACATGAACATCGACTTTGACAGCTTCATCTGCTGCTTCGTCAGGCTGGAAGGGATGTTCA
  GAGCTTTTAACGCATTTGACAAGGATGGCGATGGTATCATCAAACTGAATGTACTTGAGTGGCTGCAGCT
  TACCATGTATGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226221 representing NM_007601
  Red=Cloning site Green=Tags(s)
  
  MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPSGIYSAIISRNFPIIGVKEKTFEQLRRKCLE
  KKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTL
  NERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYA
  KLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSG
  LNMGELIARMVRNMDNSLLRDSDLDPRGSDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEK
  VKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADA
  LESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRR
  KDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPST
  YEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEGKDKAGP
  EKRGETPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSM
  IALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRY
  ADKHMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007601
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007601.3, NP_031627.2
• RefSeq Size: 3167 bp
• RefSeq ORF: 2466 bp
• Locus ID: 12335
• UniProt ID: Q64691
• Cytogenetics: 2 60.31 cM
• Gene Summary: Calcium-regulated non-lysosomal thiol-protease.[UniProtKB/Swiss-Prot Function]