Nedd4l (NM_001114386) Mouse Tagged ORF Clone

CAT#: MG225817

Nedd4l (tGFP-tagged) - Mouse neural precursor cell expressed developmentally down-regulated gene 4-like (Nedd4l) transcript variant 1, (10ug)

Product Images

Circular map for MG225817

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nedd4l
• Synonyms: 1300012C07Rik; Nedd4-2; Nedd4b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225817 representing NM_001114386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGGGCTTGGGGAGCCTGTCTACGGACTTTCGGAAGAGGAGGGAGAATCCCGTATCCTCAGAG
  TAAAAGTTGTTTCTGGAATTGACCTCGCCAAGAAGGACATATTTGGAGCCAGCGACCCATACGTGAAGCT
  GTCCTTGTATGTAGCTGATGAGAATAGAGAACTTGCTCTGGTTCAAACCAAGACAATTAAAAAGACGCTG
  AACCCAAAGTGGAATGAAGAGTTTTATTTTAGGGTAAATCCATCTAATCACAGGCTCCTATTTGAAGTAT
  TTGACGAGAACAGATTGACACGAGACGACTTCCTGGGCCAGGTGGACGTGCCTCTTAGTCACCTTCCGAC
  AGAAGATCCAACCATGGAGAGACCCTATACATTTAAGGATTTTCTCCTGCGACCTAGAAGTCATAAATCT
  CGAGTCAAGGGGTTTTTGAGGTTGAAAATGGCCTACATGCCGAAAAACGGAGGTCAGGATGAAGAAAACA
  GCGAGCAGAGGGATGACATGGAGCATGGATGGGAAGTGGTTGACTCCAACGACTCAGCTTCCCAGCACCA
  GGAGGAGCTCCCCCCTCCTCCCCTGCCGCCAGGATGGGAAGAGAAAGTGGACAATTTAGGCCGAACTTAC
  TATGTCAACCACAACAACAGGAGTACTCAGTGGCACCGACCCAGCTTGATGGATGTGTCCTCGGAATCAG
  ACAATAACATCAGGCAGATCAACCAGGAGGCTGCACACCGACGCTTCCGCTCTCGGAGGCACATTAGTGA
  AGATTTGGAGCCTGAGGCCTCTGAAGGCGGTGGAGAAGGCCCTGAGCCTTGGGAGACCATTTCAGAGGAA
  ATGAACATGGCAGGAGATTCTCTCAGCCTGGCTCTGCCCCCACCGCCTGCCTCCCCAGTGTCCCGGACCA
  GCCCCCAGGAGCTGTCGGAAGAAGTGAGCCGAAGGTTGCAGATCACTCCGGACTCCAACGGGGAACAGTT
  CAGTTCTCTGATTCAGAGAGAGCCCTCGTCAAGGCTTCGGTCCTGCAGTGTTACCGACACGGTTGCTGAG
  CAAGCTCACCTTCCACCGCCCAGCACCCCAACTAGGCGAGCCCGTTCGTCAACTGTCACGGGTGGTGAGG
  AATCCACGCCATCAGTGGCCTATGTACATACCACGCCGGGCCTGCCTTCAGGCTGGGAAGAAAGAAAGGA
  TGCAAAGGGACGCACATACTATGTCAATCATAACAATCGAACCACAACTTGGACTCGGCCAATCATGCAG
  CTTGCAGAAGACGGTGCCTCCGGATCAGCCACAAACAGTAACAACCACCTAGTCGAACCCCAGATCCGCC
  GGCCCCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCACTGGAGGGTGCCAAGGATTCACCCAT
  CCGCCGTGCCGTGAAAGATACCCTTTCCAATCCACAGTCCCCTCAGCCATCACCTTACAACTCCCCCAAA
  CCACAACACAAAGTCACACAGAGCTTCCTGCCACCAGGCTGGGAGATGAGGATAGCCCCCAACGGCCGAC
  CCTTCTTCATTGACCATAACACAAAGACTACAACCTGGGAAGATCCACGGTTGAAATTTCCAGTACACAT
  GCGGTCAAAAGCATCTTTAAACCCCAATGACCTGGGCCCTCTTCCTCCTGGCTGGGAAGAGAGGATCCAC
  TTGGATGGCCGCACGTTTTACATTGACCATAATAGTAAAATTACCCAGTGGGAAGATCCAAGACTACAGA
  ACCCAGCCATCACTGGTCCGGCTGTTCCGTACTCCAGAGAGTTTAAGCAGAAATACGACTACTTTAGGAA
  GAAATTAAAGAAGCCTGCTGATATTCCAAACAGGTTTGAAATGAAACTTCACAGAAACAACATATTTGAA
  GAGTCCTATCGGAGGATCATGTCTGTAAAGAGACCTGACGTCCTAAAGGCTAGGCTGTGGATTGAGTTTG
  AATCAGAGAAAGGCCTGGACTATGGGGGCGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTT
  TAACCCCTACTATGGCCTCTTCGAGTACTCTGCCACGGACAACTACACACTTCAGATCAATCCCAACTCA
  GGCCTCTGTAATGAAGACCATTTGTCCTATTTCACCTTCATTGGAAGAGTTGCTGGCCTAGCGGTGTTTC
  ATGGGAAACTCTTAGATGGATTCTTCATTCGACCATTCTACAAGATGATGCTGGGGAAGCAGATAACGCT
  GAACGACATGGAGTCCGTGGACAGCGAGTACTACAACTCTTTGAAGTGGATCTTAGAAAACGACCCCACG
  GAACTTGACCTCATGTTCTGCATAGACGAAGAGAACTTTGGGCAGACATACCAAGTGGATCTGAAGCCCA
  ACGGGTCAGAAATAATGGTAACCAATGAGAACAAACGAGAATACATTGACTTAGTCATCCAGTGGAGATT
  TGTGAACAGGGTCCAGAAGCAAATGAATGCCTTCTTGGAGGGATTTACAGAACTTCTTCCAATCGACTTG
  ATTAAAATTTTTGATGAAAATGAGCTGGAGTTGCTGATGTGCGGCCTTGGTGATGTCGACGTGAACGACT
  GGAGACAGCACTCTATTTACAAGAACGGCTACTGCCCCAACCACCCTGTCATCCAGTGGTTCTGGAAGGC
  CGTGCTCCTGATGGATGCTGAGAAGCGCATCCGGTTACTACAGTTTGTCACAGGCACCTCCAGAGTACCC
  ATGAATGGATTTGCCGAACTCTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTC
  CCGAAAAACTACCCAGAGCTCATACATGCTTTAATCGCCTTGATTTACCTCCATATGAAACCTTTGAAGA
  TTTACGGGAGAAACTTCTCATGGCTGTGGAAAACGCTCAAGGCTTCGAAGGTGTGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225817 representing NM_001114386
  Red=Cloning site Green=Tags(s)
  
  MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTL
  NPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKS
  RVKGFLRLKMAYMPKNGGQDEENSEQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTY
  YVNHNNRSTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWETISEE
  MNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDTVAE
  QAHLPPPSTPTRRARSSTVTGGEESTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQ
  LAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPK
  PQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIH
  LDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFE
  ESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNS
  GLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPT
  ELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDL
  IKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVP
  MNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114386
• ORF Size: 2928 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114386.1, NP_001107858.1
• RefSeq Size: 8163 bp
• RefSeq ORF: 2931 bp
• Locus ID: 83814
• Cytogenetics: 18 E1