Sele (NM_011345) Mouse Tagged ORF Clone
CAT#: MG225755
- TrueORF®
Sele (tGFP-tagged) - Mouse selectin endothelial cell (Sele), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_011345" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Sele |
Synonyms | CD62E; E-selectin; Elam; ELAM-1; LECAM2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225755 representing NM_011345
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCAACTGCTGGAGTCATGAATGCCTCGCGCTTTCTCTCTGCTCTTGTTTTTGTTCTCCTCGCTG GAGAGAGCACAGCTTGGTACTACAATGCCTCCAGTGAGCTCATGACGTATGATGAAGCCAGTGCATACTG TCAGCGGGACTACACACATCTGGTGGCAATTCAGAACAAGGAAGAGATCAACTACCTTAACTCCAATCTG AAACATTCACCGAGTTACTACTGGATTGGAATCAGAAAAGTCAATAACGTATGGATCTGGGTGGGGACGG GGAAGCCTCTGACAGAGGAAGCTCAGAACTGGGCTCCAGGTGAACCAAACAACAAACAAAGAAATGAGGA CTGTGTAGAGATTTACATCCAACGAACCAAAGACTCGGGCATGTGGAATGACGAGAGATGTAACAAAAAG AAGCTGGCTCTGTGCTACACAGCTTCGTGTACCAATGCATCCTGCAGTGGTCATGGTGAATGCATAGAGA CCATCAATAGTTACACCTGCAAGTGCCACCCTGGCTTCCTGGGACCCAACTGTGAGCAAGCTGTGACTTG CAAACCACAGGAACACCCTGACTATGGAAGCCTGAACTGCTCCCACCCGTTCGGCCCCTTCAGCTATAAT TCCTCCTGCTCCTTTGGCTGTAAAAGGGGCTACCTGCCCAGCAGCATGGAGACCACCGTGCGGTGTACGT CCTCTGGAGAGTGGAGTGCGCCTGCTCCAGCCTGCCATGTGGTTGAATGTGAAGCTTTGACCCACCCTGC CCACGGTATCAGGAAATGTTCCTCAAATCCTGGGAGCTACCCATGGAACACGACATGCACGTTTGACTGT GTGGAAGGGTACAGGCGAGTTGGAGCTCAGAATCTACAGTGTACCTCATCTGGCATCTGGGATAACGAGA CGCCATCATGCAAAGCTGTGACCTGTGACGCCATCCCTCAGCCTCAGAATGGCTTTGTGAGCTGCAGCCA CTCAACAGCTGGAGAACTTGCGTTTAAGTCATCCTGTAACTTCACCTGTGAGCAGAGTTTCACGTTGCAG GGGCCAGCGCAGGTTGAATGCAGCGCACAAGGGCAGTGGACACCACAAATCCCAGTCTGCAAAGCTGTCC AGTGTGAAGCCTTATCTGCGCCACAGCAGGGCAACATGAAATGTCTTCCCAGTGCTTCTGGACCTTTCCA AAATGGGTCCAGTTGTGAGTTCTCCTGCGAAGAAGGATTTGAACTGAAGGGATCAAGAAGACTTCAGTGT GGTCCAAGAGGGGAATGGGATAGCAAGAAGCCCACGTGTTCAGCTGTGAAATGTGATGATGTCCCTCGGC CCCAGAATGGCGTCATGGAGTGTGCTCATGCTACTACTGGAGAATTCACCTACAAGTCCTCATGTGCCTT TCAATGCAATGAGGGCTTTAGCTTGCATGGCTCAGCTCAACTTGAGTGCACATCTCAGGGAAAGTGGACC CAGGAAGTCCCCTCCTGCCAAGTGGTACAATGTCCAAGCCTTGACGTCCCGGGAAAGATGAACATGAGCT GCAGCGGAACAGCAGTTTTCGGCACAGTGTGTGAGTTTACATGTCCTGATGATTGGACACTCAATGGATC TGCAGTTCTGACGTGTGGTGCCACGGGACGCTGGTCTGGGATGCCGCCTACCTGTGAAGCCCCAGTCAGC CCCACCCGTCCCTTGGTAGTTGCACTTTCTGCGGCAGGAACCTCACTCCTGACATCGTCCTCATTGCTCT ACTTGTTGATGAGATACTTTCGGAAGAAAGCAAAGAAATTTGTTCCTGCTAGCAGCTGCCAAAGCCTTCA ATCGTTTGAAAACTACCATGTGCCTTCTTACAACGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225755 representing NM_011345
Red=Cloning site Green=Tags(s) MKATAGVMNASRFLSALVFVLLAGESTAWYYNASSELMTYDEASAYCQRDYTHLVAIQNKEEINYLNSNL KHSPSYYWIGIRKVNNVWIWVGTGKPLTEEAQNWAPGEPNNKQRNEDCVEIYIQRTKDSGMWNDERCNKK KLALCYTASCTNASCSGHGECIETINSYTCKCHPGFLGPNCEQAVTCKPQEHPDYGSLNCSHPFGPFSYN SSCSFGCKRGYLPSSMETTVRCTSSGEWSAPAPACHVVECEALTHPAHGIRKCSSNPGSYPWNTTCTFDC VEGYRRVGAQNLQCTSSGIWDNETPSCKAVTCDAIPQPQNGFVSCSHSTAGELAFKSSCNFTCEQSFTLQ GPAQVECSAQGQWTPQIPVCKAVQCEALSAPQQGNMKCLPSASGPFQNGSSCEFSCEEGFELKGSRRLQC GPRGEWDSKKPTCSAVKCDDVPRPQNGVMECAHATTGEFTYKSSCAFQCNEGFSLHGSAQLECTSQGKWT QEVPSCQVVQCPSLDVPGKMNMSCSGTAVFGTVCEFTCPDDWTLNGSAVLTCGATGRWSGMPPTCEAPVS PTRPLVVALSAAGTSLLTSSSLLYLLMRYFRKKAKKFVPASSCQSLQSFENYHVPSYNV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011345 |
ORF Size | 1857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011345.2, NP_035475.1 |
RefSeq Size | 2912 bp |
RefSeq ORF | 1860 bp |
Locus ID | 20339 |
UniProt ID | Q00690 |
Cytogenetics | 1 71.35 cM |
Gene Summary | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1 (PubMed:1375914). May have a role in capillary morphogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219711 | Sele (untagged) - Mouse selectin, endothelial cell (Sele), (10ug) |
USD 577.00 |
|
MR225755 | Sele (Myc-DDK-tagged) - Mouse selectin, endothelial cell (Sele) |
USD 576.00 |
|
MR225755L3 | Lenti ORF clone of Sele (Myc-DDK-tagged) - Mouse selectin, endothelial cell (Sele) |
USD 876.00 |
|
MR225755L4 | Lenti ORF clone of Sele (mGFP-tagged) - Mouse selectin, endothelial cell (Sele) |
USD 876.00 |
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