Cd22 (NM_001043317) Mouse Tagged ORF Clone
CAT#: MG225294
- TrueORF®
Cd22 (tGFP-tagged) - Mouse CD22 antigen (Cd22) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001043317" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Cd22 |
Synonyms | A530093D23; Lyb-8; Lyb8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225294 representing NM_001043317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGTCCATTACCTGTGGCTCCTCTTGATCCTAGGACACGTGGCTTCGGCTCGGTACAGCTCAGCAA ATGATTGGACCGTTGACCATCCCCAAACCCTCTTTGCCTGGGAGGGAGCCTGCATCAGGATTCCTTGCAA GTACAAAACTCCACTACCCAAGGCACGTCTGGACAACATCCTCCTTTTTCAGAACTATGAGTTTGACAAG GCCACCAAGAAATTCACAGGAACTGTCCTGTACAACGCCACAAAGACTGAGAAGGACCCAGAGTCTGAGC TGTACCTTTCTAAGCAAGGGAGAGTAACATTTCTGGGGAACAGAATAGACAATTGTACCCTGAAAATCCA CCCGATACGTGCCAATGACAGTGGGAATCTGGGGTTGAGGATGACCGCAGGGACTGAACGATGGATGGAG CCCATTCACCTCAATGTCTCGGAGAAACCGTTCCAACCTTACATCCAGATGCCATCAGAAATCCGGGAAT CCCAAAGTGTCACTCTGACCTGTGGACTGAATTTCTCCTGCTTTGGGTATGACATCCTCTTGAAGTGGTT CCTAGAAGATTCTGAGATCACCTCCATCACCTCTTCTGTCACCTCCATCACCTCTTCTGTCACCTCCTCC ATTAAGAATGTCTATACAGAGAGCAAGCTCACCTTCCAACCAAAGTGGACGGACCACGGAAAGAGTGTGA AGTGCCAAGTCCAGCACTCCTCCAAAGTGCTCTCAGAGTGCACTGTGCATCTGGATGTTAAGTATACCCC GAAGCTGGAGATCAAGGTCAATCCCACAGAGGTGGAAAAGAACAATTCTGTGACCATGACATGCCGGGTT AACAGCAGCAACCCGAAACTCAGGACCGTGGCGGTGTCTTGGTTCAAGGATGGGCGCCCCCTAGAGGATC AGGAACTGGAACAGGAACAACAGATGTCCAAGCTAATTCTGCATTCAGTGACCAAGGACATGAGAGGGAA ATACCGGTGCCAGGCTTCCAACGACATAGGCCCAGGAGAGTCGGAAGAAGTGGAACTCACGGTGCACTAT GCTCCAGAACCCTCCAGGGTTCACATCTACCCTTCCCCCGCTGAAGAGGGACAGTCAGTAGAGCTGATTT GTGAGTCACTGGCCAGTCCAAGTGCAACAAACTACACCTGGTATCACAACAGGAAACCTATACCTGGAGA CACCCAAGAGAAGCTCCGCATCCCTAAAGTCTCCCCGTGGCATGCTGGGAATTACTCTTGCTTGGCAGAG AACCGTCTGGGTCATGGAAAGATAGACCAGGAAGCTAAGCTGGATGTCCATTATGCTCCCAAGGCGGTGA CCACAGTGATTCAGAGCTTCACACCAATCCTGGAAGGAGACAGTGTGACCCTGGTCTGTAGGTACAACTC CAGCAATCCAGACGTCACCTCCTACAGATGGAACCCTCAAGGTTCTGGGAGTGTGCTCAAACCCGGAGTG CTGAGGATTCAGAAAGTGACATGGGATTCCATGCCTGTCAGCTGTGCTGCCTGCAACCACAAGTGTTCGT GGGCCCTCCCTGTCATCCTGAATGTCCACTACGCCCCCAGAGACGTGAAGGTACTGAAGGTAAGCCCCGC ATCAGAGATCCGCGCTGGGCAGCGTGTCCTCCTCCAATGCGACTTCGCAGAGAGCAACCCGGCAGAGGTC CGCTTCTTCTGGAAGAAGAATGGGAGTCTCGTGCAGGAAGGGAGGTACCTGAGCTTCGGCTCCGTCTCCC CAGAAGATTCTGGAAATTATAACTGCATGGTCAACAACTCCATCGGAGAGACCTTGTCACAGGCCTGGAA CCTCCAAGTGCTGTATGCTCCTCGGAGGCTGCGTGTGTCCATCAGCCCTGGGGACCATGTGATGGAGGGG AAGAAGGCCACCTTGTCCTGTGAGAGTGATGCCAATCCGCCCATCTCACAGTACACCTGGTTTGACTCCA GTGGCCAAGACCTCCACTCCTCAGGCCAGAAACTGAGACTGGAACCCCTGGAGGTCCAACACACGGGTTC CTACCGCTGCAAAGGGACCAATGGGATAGGCACAGGAGAGTCACCACCCAGCACCCTCACTGTCTACTAC AGTCCAGAGACCATCGGCAAGCGGGTCGCCTTGGGACTAGGCTTCTGCCTGACTATCTTCATCCTGGCCA TCTGGGGGATGAAAATCCAGAAAAAATGGAAGCAAAACCGCAGCCAGCAGGGGCTTCAGGAAAATTCCAG TGGCCAGAGCTTTTTTGTGAGGAACAAAAAGGCTAGGAGGACCCCTCTCTCAGAAGGCCCCCAATCCCAG GGATGCTACAATCCGGCAATGGATGACACTGTTAGTTATGCCATCTTGCGCTTTCCAGAGAGTGACACGC ACAATGCTGGAGATGCAGGGACCCCAGCAACACAGGCTCCTCCTCCAAACAACAGCGACACGGTCACTTA CTCGGTGATACAGAAGCGGCCTATGGGGGATTATGAGAATGTGAATCCGAGCTGCCCGGAGGATGAGAGC ATCCATTACTCAGAGCTGGTTCAGTTTGGGGCTGGTAAGCGGCCCCAGGCAAAGGAAGACGTAGACTATG TGACCCTCAAGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225294 representing NM_001043317
Red=Cloning site Green=Tags(s) MRVHYLWLLLILGHVASARYSSANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDK ATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWME PIHLNVSEKPFQPYIQMPSEIRESQSVTLTCGLNFSCFGYDILLKWFLEDSEITSITSSVTSITSSVTSS IKNVYTESKLTFQPKWTDHGKSVKCQVQHSSKVLSECTVHLDVKYTPKLEIKVNPTEVEKNNSVTMTCRV NSSNPKLRTVAVSWFKDGRPLEDQELEQEQQMSKLILHSVTKDMRGKYRCQASNDIGPGESEEVELTVHY APEPSRVHIYPSPAEEGQSVELICESLASPSATNYTWYHNRKPIPGDTQEKLRIPKVSPWHAGNYSCLAE NRLGHGKIDQEAKLDVHYAPKAVTTVIQSFTPILEGDSVTLVCRYNSSNPDVTSYRWNPQGSGSVLKPGV LRIQKVTWDSMPVSCAACNHKCSWALPVILNVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESNPAEV RFFWKKNGSLVQEGRYLSFGSVSPEDSGNYNCMVNNSIGETLSQAWNLQVLYAPRRLRVSISPGDHVMEG KKATLSCESDANPPISQYTWFDSSGQDLHSSGQKLRLEPLEVQHTGSYRCKGTNGIGTGESPPSTLTVYY SPETIGKRVALGLGFCLTIFILAIWGMKIQKKWKQNRSQQGLQENSSGQSFFVRNKKARRTPLSEGPQSQ GCYNPAMDDTVSYAILRFPESDTHNAGDAGTPATQAPPPNNSDTVTYSVIQKRPMGDYENVNPSCPEDES IHYSELVQFGAGKRPQAKEDVDYVTLKH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001043317 |
ORF Size | 2604 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001043317.2, NP_001036782.1 |
RefSeq Size | 4097 bp |
RefSeq ORF | 2607 bp |
Locus ID | 12483 |
UniProt ID | P35329 |
Cytogenetics | 7 19.26 cM |
Gene Summary | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222250 | Cd22 (untagged) - Mouse CD22 antigen (Cd22), transcript variant 1, (10ug) |
USD 1,193.00 |
|
MR225294 | Cd22 (Myc-DDK-tagged) - Mouse CD22 antigen (Cd22), transcript variant 1 |
USD 1,192.00 |
|
MR225294L3 | Lenti ORF clone of Cd22 (Myc-DDK-tagged) - Mouse CD22 antigen (Cd22), transcript variant 1 |
USD 1,492.00 |
|
MR225294L4 | Lenti ORF clone of Cd22 (mGFP-tagged) - Mouse CD22 antigen (Cd22), transcript variant 1 |
USD 1,492.00 |
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