Brd4 (NM_020508) Mouse Tagged ORF Clone

CAT#: MG224909

Brd4 (tGFP-tagged) - Mouse bromodomain containing 4 (Brd4) transcript variant 1, (10ug)

Product Images

Circular map for MG224909

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Brd4
• Synonyms: Brd5; HUNK1; MCAP; WI-11513
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224909 representing NM_020508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCT
  CCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACC
  CCCAGAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATATCTGCTCAGAGTGGTG
  CTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACC
  TCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAA
  CTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAAC
  AAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAAC
  TGCCTACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAGGGAGGAAAGAAACAGG
  GGCAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAGACG
  CCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACCTCA
  TTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTTCAGACTCCTTCACCGGTACCCCCCCA
  GCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACCCCC
  CAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCTACCACCATCGACCCCA
  TTCATGAGCCACCCTCACTGGCCCCAGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAG
  ACCTGTGAAGCCTCCAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGCAGCAAG
  ATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCCAAGAAACATGCTGCCTATG
  CCTGGCCTTTCTACAAGCCTGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAAACA
  TCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGATGCCCAGGAATTTGGT
  GCTGATGTCCGATTGATGTTCTCCAACTGCTACAAGTACAACCCCCCTGACCATGAAGTGGTAGCCATGG
  CTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGTTGT
  TACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGCAGC
  AGCGACAGTTCTTCCGACAGCGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTG
  AACTCCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAGCAGAACAAACC
  AAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAGGAA
  AATAAAAAAAGCAAAACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCAATG
  TGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAGGAGGATAA
  GTGTAAGCCCATGTCTTATGAGGAGAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAG
  CTAGGCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCCAACCCCGATGAGATTG
  AGATTGACTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCG
  GAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGATTCTCA
  TCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAGTGAAGACTCTGAAACAGAGATGGCTC
  CCAAGTCAAAAAAGAAGGGGCACACTGGGAGGGACCAGAAAAAGCATCATCACCATCACCATCCACAGAT
  GCAGCCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCCCCACCTCCACAGCAGCCTCCTCCACCCCCACCT
  CCGCAGCAGCAGCAGCAGCAACCTCCACCCCCACCACCTCCGCCCTCCATGCCACAGCAGACTGCCCCAG
  CGATGAAGTCCTCGCCCCCACCCTTCATCACTGCCCAGGTCCCCGTCCTGGAACCACAGCTGCCAGGCAG
  TGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACCTGCCGCAGCCGGAGCTGCCTCCTCAC
  CTGCCCCAGCCACCTGAGCACAGCACTCCACCCCATCTCAACCAGCATGCTGTGGTCTCTCCTCCAGCTT
  TGCACAATGCGCTGCCCCAACAGCCATCTCGGCCCAGTAACCGAGCTGCTGCTCTGCCCCCAAAGCCTAC
  CCGACCCCCAGCTGTGTCCCCTGCCCTGGCCCAGCCCCCCCTGCTCCCACAACCACCAATGGCTCAGCCC
  CCCCAAGTGCTGCTGGAGGATGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGTTGTACC
  TACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCTTCCGTGAAGGTGCAGTCCCAACCCCC
  ACCGCCTTTGCCGCCTCCACCCCACCCTTCTGTGCAGCAGCAGCAGCTCCAGCCACAGCCACCGCCACCC
  CCACCTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCTCCACCACGACCAGTTCACTTGCCAT
  CCATGCCCTTTTCTGCTCATATTCAGCAGCCCCCACCACCCCCAGGACAGCAACCTACTCACCCACCCCC
  AGGACAGCAGCCCCCACCACCACAGCCTGCCAAGCCCCAGCAAGTCATCCAGCATCACCCTTCCCCCCGG
  CACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAGGCTCCCTCTCCGCTTATGATACATTCCC
  CTCAGATGCCACAGTTCCAGAGCCTGACCCATCAGTCTCCTCCCCAGCAAAACGTCCAGCCTAAGAAGCA
  GGTAAAGGGCAGGGCTGAGCCACAGCCACCAGGGCCAGTCATGGGCCAAGGCCAGGGATGCCCACCTGCC
  TCACCGGCTGCCGTGCCTATGCTGTCCCAGGAGCTGCGACCACCTTCAGTCGTCCAGCCCCAGCCCCTGG
  TGGTAGTAAAGGAGGAGAAGATTCACTCACCAATCATTCGCAGCGAGCCTTTCAGCACCTCACTTCGACC
  AGAGCCCCCCAAGCACCCAGAGAACATCAAGGCCCCAGTCCACCTGCCCCAGCGGCCTGAGATGAAGCCT
  GTAGACATAGGGAGGCCTGTGATCCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGGCCCCTGACAAGG
  ACAAACAGAAGCAGGAGCCAAAAACACCAGTGGCACCCAAAAAGGACCTGAAAATTAAGAACATGGGCTC
  CTGGGCCAGCCTGGTACAGAAGCATCCGACCACCCCATCCTCCACAGCCAAGTCCTCAAGTGACAGCTTT
  GAGCATTTCCGCCGTGCTGCTCGGGAGAAGGAGGAGAGGGAAAAGGCCCTGAAGGCTCAGGCTGAGCATG
  CAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAGCCGAGAGGATGAAGATGCGCTGGAACA
  GGCACGTCGAGCCCATGAGGAGGCACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGAGACAG
  GAGCAGCAGCAGCAGCAGCAGCAGGCAGCTGCTGTGGCCGCTGCCTCTGCCCCCCAGGCCCAGAGCTCCC
  AGCCCCAGTCTATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAACGAGAGCAGGAGCGGAGGCGCAGGGA
  GGCAATGGCAGCTACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAAAATCTTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224909 representing NM_020508
  Red=Cloning site Green=Tags(s)
  
  MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVV
  LKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYN
  KPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQT
  PQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTP
  QPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSK
  ISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFG
  ADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSS
  SDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEE
  NKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEK
  LGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFS
  SSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPP
  PQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPH
  LPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQP
  PQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPP
  PPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPR
  HHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPA
  SPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKP
  VDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSF
  EHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQ
  EQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020508
• ORF Size: 4200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020508.4, NP_065254.3
• RefSeq Size: 5948 bp
• RefSeq ORF: 4203 bp
• Locus ID: 57261
• UniProt ID: Q9ESU6
• Cytogenetics: 17 17.39 cM
• Gene Summary: This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008]