Ak2 (NM_001033966) Mouse Tagged ORF Clone
CAT#: MG224825
- TrueORF®
Ak2 (tGFP-tagged) - Mouse adenylate kinase 2 (Ak2) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001033966" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ak2 |
Synonyms | Ak-2; D4Ertd220e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224825 representing NM_001033966
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCAACGTGTTGGCTTCCGAACCGGAGATTCCGAAGGGCATCCGGGCCGTGTTGCTGGGGCCGC CCGGGGCTGGCAAGGGGACGCAGGCACCCAAACTGGCTGAAAACTTTTGTGTCTGTCATTTGGCCACCGG GGACATGCTGAGAGCCATGGTAGCTTCTGGCTCAGAGCTAGGAAAAAAGCTGAAGGCGACAATGGATGCA GGGAAACTGGTGAGTGACGAAATGGTTGTGGAGCTAATTGAGAAGAATTTGGAGACTCCTTCGTGCAAAA ATGGCTTTCTTCTAGATGGCTTCCCTCGGACTGTGAGGCAGGCTGAAATGCTTGATGACCTCATGGAGAA GAGGAAAGAGAAGCTCGATTCAGTCATTGAGTTCAGCATCCAAGACTCGCTGCTGATCCGGAGGATCACT GGGAGGCTGATTCACCCCAAGAGTGGCCGGTCCTACCATGAGGAATTCAACCCTCCAAAGGAGCCCATGA AAGATGATATCACTGGGGAGCCCCTGATCCGCAGGTCAGATGACAACGAGAAAGCCTTGAAGACCCGCCT GGAGGCCTACCACACTCAGACCACTCCGCTCGTGGAGTACTACAGGAAACGCGGCATTCACTGCGCCATC GATGCGTCCCAGACCCCTGACATCGTGTTTGCGAGCATCCTGGCAGCCTTCTCCAAAGCCACATGTAAAG ACCTGGTCATGTTTATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224825 representing NM_001033966
Red=Cloning site Green=Tags(s) MAPNVLASEPEIPKGIRAVLLGPPGAGKGTQAPKLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDA GKLVSDEMVVELIEKNLETPSCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIQDSLLIRRIT GRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKTRLEAYHTQTTPLVEYYRKRGIHCAI DASQTPDIVFASILAAFSKATCKDLVMFI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033966 |
ORF Size | 717 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033966.4, NP_001029138.1 |
RefSeq Size | 1308 bp |
RefSeq ORF | 720 bp |
Locus ID | 11637 |
UniProt ID | Q9WTP6 |
Cytogenetics | 4 62.62 cM |
Gene Summary | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224825 | Ak2 (Myc-DDK-tagged) - Mouse adenylate kinase 2 (Ak2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 330.00 |
|
MR224825L3 | Lenti ORF clone of Ak2 (Myc-DDK-tagged) - Mouse adenylate kinase 2 (Ak2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 630.00 |
|
MR224825L4 | Lenti ORF clone of Ak2 (mGFP-tagged) - Mouse adenylate kinase 2 (Ak2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 630.00 |
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