Arntl2 (NM_172309) Mouse Tagged ORF Clone

CAT#: MG224577

Arntl2 (tGFP-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like 2 (Arntl2), (10ug)

Product Images

Circular map for MG224577

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arntl2
• Synonyms: 4632430A05Rik; bHLHe6; BMAL2; CLIF; MOP9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224577 representing NM_172309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTCCAAGGAAACGCAGAGGCAGAGATTCCCAGCCACTCCAGTCAGAATTCATGACAGACACAA
  CAGTGGAAAGTCTTCCCCAGAATCCCTTTGCCTCTCTTCTTTCAACAAGAACAGGAGTATCAGCGCCCAG
  TGGCATCAGGGAAGCTCACAGCCAGATGGAAAAGCGTCGGAGAGACAAGATGAACCATCTGATTCAGAAA
  CTGTCATCTATGATCCCTCCACACATCCCCACGGCCCACAAACTGGACAAGCTCAGCGTCTTGAGGAGGG
  CGGTGCAGTACTTGAGGTCTCTGAGAGGCATGACAGAGCTTTACTTAGGAGAAAACTCTAAACCTTCATT
  TATTCAGGATAAGGAACTCAGTCACTTAATCCTCAAGGCAGCAGAAGGCTTCCTGTTTGTGGTTGGATGC
  GAAAGAGGGAGAATTTTTTACGTTTCTAAGTCTGTCTCCAAAACACTGCGTTATGATCAGGCTAGCTTGA
  TAGGACAGAATTTGTTTGACTTCTTACACCCAAAAGACGTCGCCAAAGTAAAGGAACAACTTTCTTGTGA
  TGGTTCACCAAGAGAGAAACCTATAGACACCAAAACCTCTCAGGTTTACAGTCACCCCTACACTGGGCGA
  CCACGCATGCATTCTGGCTCCAGACGATCTTTCTTCTTTAGAATGAAGAGCTGTACCGTCCCTGTCAAAG
  AAGAGCAGCCATGCTCGTCCTGCTCAAAGAAGAAAGACCATAGAAAATTCCACACCGTCCATTGCACTGG
  ATACTTGAGAAGCTGGCCTCTGAATGTTGTTGGCATGGAGAAAGAGTCGGGTGGTGGGAAGGACAGCGGT
  CCTCTTACCTGCCTTGTGGCTATGGGACGGTTGCATCCATACATTGTCCCTCAAAAGAGTGGCAAGATCA
  ACGTGAGACCGGCTGAGTTCATAACTCGCTTCGCAATGAACGGGAAATTCGTCTATGTTGACCAAAGGGC
  AACGGCAATTTTAGGATACCTGCCTCAGGAACTTTTGGGAACTTCATGTTATGAATATTTTCATCAGGAT
  GACCACAGTAGTTTGACTGACAAGCACAAAGCAGTTCTGCAGAGTAAGGAGAAAATACTTACAGACTCAT
  ACAAATTCAGAGTGAAGGATGGTGCCTTCGTGACTCTGAAGAGTGAGTGGTTCAGCTTCACAAACCCTTG
  GACCAAAGAGCTGGAGTACATTGTGTCTGTCAACACATTGGTTTTGGGGCGCAGTGAGACCAGGCTGTCT
  TTGCTTCATTGCGGCGGCAGCAGCCAGTCCTCCGAAGACTCATTTAGACAATCCTGCATCAATGTGCCCG
  GTGTATCCACGGGGACCGTCCTTGGTGCTGGGAGTATTGGAACAGATATTGCAAATGAGGTTCTGAGTTT
  ACAGAGATTACACTCTTCATCCCCAGAAGATGCAAGCCCTTCAGAAGAAGTGAGAGATGACTGCAGTGTA
  AATGGTGGGAATGCCTATGGGCCTGCATCCACTAGGGAGCCTTTTGCAGTGAGCCCTTCTGAAACAGAGG
  TCCTGGAGGCTGCCAGGCAACACCAGAGCACTGAACCCGCCCACCCTCACGGACCACTTCCCGGTGACAG
  TGCCCAGCTGGGTTTTGATGTCCTGTGTGACAGTGACAGCATAGACATGGCTGCATTCATGAATTACCTC
  GAAGCAGAGGGGGGCCTGGGTGACCCTGGGGACTTCAGTGACATCCAGTGGGCACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224577 representing NM_172309
  Red=Cloning site Green=Tags(s)
  
  MEFPRKRRGRDSQPLQSEFMTDTTVESLPQNPFASLLSTRTGVSAPSGIREAHSQMEKRRRDKMNHLIQK
  LSSMIPPHIPTAHKLDKLSVLRRAVQYLRSLRGMTELYLGENSKPSFIQDKELSHLILKAAEGFLFVVGC
  ERGRIFYVSKSVSKTLRYDQASLIGQNLFDFLHPKDVAKVKEQLSCDGSPREKPIDTKTSQVYSHPYTGR
  PRMHSGSRRSFFFRMKSCTVPVKEEQPCSSCSKKKDHRKFHTVHCTGYLRSWPLNVVGMEKESGGGKDSG
  PLTCLVAMGRLHPYIVPQKSGKINVRPAEFITRFAMNGKFVYVDQRATAILGYLPQELLGTSCYEYFHQD
  DHSSLTDKHKAVLQSKEKILTDSYKFRVKDGAFVTLKSEWFSFTNPWTKELEYIVSVNTLVLGRSETRLS
  LLHCGGSSQSSEDSFRQSCINVPGVSTGTVLGAGSIGTDIANEVLSLQRLHSSSPEDASPSEEVRDDCSV
  NGGNAYGPASTREPFAVSPSETEVLEAARQHQSTEPAHPHGPLPGDSAQLGFDVLCDSDSIDMAAFMNYL
  EAEGGLGDPGDFSDIQWAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172309
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172309.2, NP_758513.1
• RefSeq Size: 1740 bp
• RefSeq ORF: 1740 bp
• Locus ID: 272322
• UniProt ID: Q2VPD4
• Cytogenetics: 6 G3
• Gene Summary: This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (Per, Arnt, Sim) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian Clock protein, the structurally related Mop4, and hypoxia-inducible factors, such as Hif1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]