Aifm1 (NM_012019) Mouse Tagged ORF Clone
CAT#: MG224344
- TrueORF®
Aifm1 (tGFP-tagged) - Mouse apoptosis-inducing factor mitochondrion-associated 1 (Aifm1) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_012019" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Aifm1 |
Synonyms | AIF; AIFsh2; Hq; Pdcd8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224344 representing NM_012019
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGGTGTGGAGGCCTGGCGGGTGCTTTCAAGCAGAAACTGGTGCCCTTGGTGCGGACGGTGTACG TCCAGAGGCCGAAACAGAGGAACCGGCTTCCAGGCAACTTGTTCCAGCAATGGCGTGTTCCTCTAGAACT CCAGATGGCAAGACAAATGGCTAGCTCTGGTTCATCAGGGGGCAAAATGGATAATTCTGTGTTAGTCCTT ATTGTGGGCTTATCAACAATAGGAGCTGGTGCATATGCCTACAAAACTATAAAAGAAGACCAAAAAAGAT ACAATGAAAGAGTGATGGGATTAGGACTGTCCCCAGAAGAGAAACAGAGAAGAGCCATTGCCTCCGCTAC AGAGGGAGGCTCAGTTCCTCAGATCAGGGCACCAAGTCACGTCCCTTTCCTGCTGATTGGTGGAGGGACT GCTGCTTTTGCAGCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGGGTCCTGATTGTATCTGAAG ACCCTGAACTGCCATACATGCGACCTCCTCTTTCAAAAGAATTGTGGTTTTCAGATGATCCAAATGTCAC AAAGACACTGCAATTCAGACAGTGGAATGGAAAAGAGAGAAGCATATATTTCCAGCCACCTTCTTTCTAT GTCTCTGCTCAGGACCTGCCTAATATTGAGAACGGTGGTGTGGCTGTCCTCACTGGGAAAAAGGTAGTAC ATCTGGATGTAAGAGGCAACATGGTGAAACTTAATGATGGCTCTCAGATTACCTTTGAAAAGTGCTTGAT TGCAACGGGAGGCACTCCAAGAAGTCTGTCTGCCATCGATAGGGCTGGAGCAGAGGTGAAGAGTAGAACA ACACTTTTCAGGAAGATTGGAGATTTTAGAGCCTTGGAGAAGATCTCTCGGGAGGTCAAGTCAATTACAG TTATCGGCGGGGGCTTCCTTGGGAGTGAGCTGGCCTGTGCTCTTGGCAGAAAGTCTCAAGCCTCGGGCAT AGAAGTGATCCAGCTGTTCCCTGAGAAAGGAAATATGGGGAAGATCCTTCCTCAATACCTCAGCAACTGG ACCATGGAAAAAGTCAAACGAGAGGGAGTGAAAGTGATGCCCAATGCAATTGTACAATCAGTTGGAGTCA GCGGTGGCAGGTTACTCATTAAGCTGAAAGATGGAAGGAAGGTAGAAACTGACCACATAGTGACAGCTGT GGGCCTAGAGCCCAATGTTGAGTTGGCCAAGACTGGCGGACTGGAAATAGATTCCGATTTTGGTGGCTTC CGGGTAAATGCAGAACTCCAAGCACGTTCTAACATCTGGGTGGCAGGGGATGCTGCATGCTTCTATGATA TAAAGTTGGGTCGAAGGCGAGTAGAGCATCATGATCATGCTGTTGTGAGTGGAAGACTGGCTGGAGAAAA CATGACTGGAGCCGCTAAGCCATACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGTCCTGATGTCGGC TATGAAGCTATTGGTCTGGTGGATAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAG ACAACCCAAAATCTGCCACAGAGCAGTCAGGAACTGGTATCCGTTCGGAGAGTGAGACAGAGTCAGAAGC TTCGGAAATCACAATTCCTCCCAGCGCCCCTGCAGTCCCACAGGTCCCTGTTGAAGGGGAGGACTACGGC AAAGGTGTCATCTTCTACCTCAGGGACAAAGTTGTGGTGGGGATTGTGCTATGGAACGTCTTTAACCGAA TGCCAATTGCAAGGAAGATCATTAAGGACGGTGAGCAACATGAAGATCTCAATGAAGTAGCTAAACTCTT CAACATTCATGAAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224344 representing NM_012019
Red=Cloning site Green=Tags(s) MFRCGGLAGAFKQKLVPLVRTVYVQRPKQRNRLPGNLFQQWRVPLELQMARQMASSGSSGGKMDNSVLVL IVGLSTIGAGAYAYKTIKEDQKRYNERVMGLGLSPEEKQRRAIASATEGGSVPQIRAPSHVPFLLIGGGT AAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLQFRQWNGKERSIYFQPPSFY VSAQDLPNIENGGVAVLTGKKVVHLDVRGNMVKLNDGSQITFEKCLIATGGTPRSLSAIDRAGAEVKSRT TLFRKIGDFRALEKISREVKSITVIGGGFLGSELACALGRKSQASGIEVIQLFPEKGNMGKILPQYLSNW TMEKVKREGVKVMPNAIVQSVGVSGGRLLIKLKDGRKVETDHIVTAVGLEPNVELAKTGGLEIDSDFGGF RVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVG YEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSAPAVPQVPVEGEDYG KGVIFYLRDKVVVGIVLWNVFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012019 |
ORF Size | 1836 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012019.3, NP_036149.1 |
RefSeq Size | 2115 bp |
RefSeq ORF | 1839 bp |
Locus ID | 26926 |
UniProt ID | Q9Z0X1 |
Cytogenetics | X 25.68 cM |
Gene Summary | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219720 | Aifm1 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein, (10ug) |
USD 913.00 |
|
MR224344 | Aifm1 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
USD 854.00 |
|
MR224344L3 | Lenti ORF clone of Aifm1 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
USD 1,154.00 |
|
MR224344L4 | Lenti ORF clone of Aifm1 (mGFP-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
USD 1,154.00 |
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