Upk1a (NM_026815) Mouse Tagged ORF Clone

CAT#: MG224179

Upk1a (tGFP-tagged) - Mouse uroplakin 1A (Upk1a), (10ug)

Product Images

Circular map for MG224179

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Upk1a
• Synonyms: 1110031P12Rik; AU019298; C87192; Tspan21; Upk1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224179 representing NM_026815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGCAGCGACAGAGGGAGAGAAGGGGTCTCCTGTGGTGGTGGGGCTGCTGGTCGTGGGCAACA
  TCATTATTTTGCTGTCAGGCCTGGCCCTGTTTGCGGAGACAGTGTGGGTAACAGCTGACCAGTACCGCGT
  GTACCCACTGATGGGCGTCTCAGGCAAGGATGATGTCTTCGCTGGCGCCTGGATTGCCATCTTCTGCGGC
  TTCTCCTTCTTCGTCGTGGCCAGCTTTGGTGTAGGAGCTGCGCTCTGTCGTCGTCGGTACATGATCCTCA
  CGTACCTGCTGCTGATGCTCATCGTCTACATCTTCGAGTGTGCCTCCTGCATCACATCCTACACCCACCG
  CGACTATATGGTGTCCAACCCATCCCTGATAACCAAGCAAATGTTGACCTACTACAGTGCAGACACTGAC
  CAGGGCCAGGAGCTAACCCGACTCTGGGACCGGATCATGATTGAGCAAGAGTGTTGTGGCACATCTGGCC
  CCATGGACTGGGTGAATTACACATCAGCCTTCCGGGCAGCCACCCCGGAAGTGGTGTTCCCGTGGCCCCC
  ACTGTGCTGTCGTCGGACAGGCAACTTCATCCCCATCAATGAAGATGGCTGCCGAGTAGGCCACATGGAC
  TACCTGTTTACCAAGGGTTGCTTCGAGCACATCGGCCATGCCATTGATAGCTACACGTGGGGCATATCGT
  GGTTTGGCTTTGCCATCTTGATGTGGACCCTCCCTGTGATGTTGATAGCCATGTATTTCTACACCACTCT
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224179 representing NM_026815
  Red=Cloning site Green=Tags(s)
  
  MASAATEGEKGSPVVVGLLVVGNIIILLSGLALFAETVWVTADQYRVYPLMGVSGKDDVFAGAWIAIFCG
  FSFFVVASFGVGAALCRRRYMILTYLLLMLIVYIFECASCITSYTHRDYMVSNPSLITKQMLTYYSADTD
  QGQELTRLWDRIMIEQECCGTSGPMDWVNYTSAFRAATPEVVFPWPPLCCRRTGNFIPINEDGCRVGHMD
  YLFTKGCFEHIGHAIDSYTWGISWFGFAILMWTLPVMLIAMYFYTTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026815
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026815.2, NP_081091.1
• RefSeq Size: 1308 bp
• RefSeq ORF: 774 bp
• Locus ID: 109637
• UniProt ID: Q9D132
• Cytogenetics: 7 18.78 cM
• Gene Summary: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).[UniProtKB/Swiss-Prot Function]