Herc6 (NM_025992) Mouse Tagged ORF Clone

CAT#: MG224065

Herc6 (tGFP-tagged) - Mouse hect domain and RLD 6 (Herc6), (10ug)

Product Images

Circular map for MG224065

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Herc6
• Synonyms: 1700121D12Rik; 2510038N07Rik; 4930427L17Rik; AI451296; CEB1; Herc5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224065 representing NM_025992
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTCAGCTGGGCTGCAGGAAGCCGGAAGCCTCGGCGCCTGAAGGCGGGAACCAGCGGCATTGAAC
  TCTTGCAGGCAGCCAGCGGGGAACACCACTCGCTCCTACTGTTCAGCAACCACAGGGTGTATTCCTGCGG
  GGACAACAGCTGGGGACAGCTGGGCCAGAGGAGAGACCAGAGTACCGAAAGGCCAGAACCAATTCAGGCA
  CTGAATGATCTGCATGTTGACCTGGTGAGCTGTGGGAAGGAACACTCTGTGGCTGTCTGCCACAAAGGAA
  AGGTCTTCGCTTGGGGAGCCGGTTCTGAAGGGCAGCTGGGGATTGGAGAATTTAAGGAAATAAGCTTTAT
  GCCTACGAAAATAAAAGCTCTGGCTGGCATAAAAATAATCCAAGTTTCCTGTGGACACTACCACTCTTTG
  GCCTTATCAGAAGATGGCCACGTGTTTTCATGGGGAAGGAACAGTGAAGGACAACTGGGCCTGGGGAAGA
  ATTCCCGTTCTCAAGCTATTCCACAGAAGGTGAAGTCTCTGGAGGGGATCCCACTGGCTCAGGTTGCTGC
  AGGTGGCACACACAGTTTTGCTCTGTCTCTCACGGGGACATCATTTGGCTGGGGGAGCAACAGATCTGGG
  CAGTTGGCTCTCAGCGGGAACAAAGTTAAAGAACAAATCTACAAGCCGCATTCAATTGGTGCCCTGAAGA
  ATCTGAGTGTGATTTATATTAGCTGTGGTTATGAGCATACTGCAGTGCTTACTGAGGAGGGACAAGTGTT
  TACATTTGGAGGCAATAGCTCTGGACAACTACAGCCCAGCCCCAGGAGTGGACAAAGAGGCCCACAACTC
  ATAGAAGGAATTGGTGGCCGTGTTTCACAGATAGAGTGCGCAAGTTATCACACCATTGCATATGTCTACA
  CCACTGGTCAGGTGGTATCTTTAGGCCGTGGACCAAGTCACACAAGCAACCCAACTCATCAGGAGGCCCC
  CGCAGAGAACTCTGATATTACCTGCCTGCTAGCTGCAGAGGACCTTGTGGATATTGAAGTCAAAGACATT
  TTTGCTGGAGCACACGCCAACTTTGTGACAACTCGTCGGGTATCACATACTAGGTCTACAGGTGTTTCCA
  TGAAAATCCTGCCAGAAATAATCCGAATCAACCAGTCTCTGGTAAAGAAATGGAGAGCAGCAAATAAAAG
  AAAAGATCGTGAAGGCGCCAAAAGAGAAATCAGCTTGATATTTTCATCTTCTGCTTGTTTGACAGCGAGC
  TTTCTGAAGAAAAGAGATGCTGGAGAGAATAATCTCATTGATGTGGACTTAAAACTGGCAAGAGATGTCT
  TCAAGAAGTTAACAACAGAGAAATGGATTTCTTCTTTGATAACCACGTGTCTTGAGGAGTATCTGCTCAG
  AGATCTTCCATACAGTTCTCCACACCAAGAAGCTCTACTTGTTTTTCTTTTGCTCCCAGAATGTTCCATT
  ATGCAGGATCCTAAGAACTGGAAGACCCTGGCATTTGAATTTGCAAAAGCTATTCATAAAATGGGTCCAC
  AGTCATTAGCATTCCTGAGAACGTGTTGGGCATCTTTGGAAGTTTCTTCACTGAACATACTGGTTCAGAT
  GCTAAAGAAAGCCATCATCTCTCAAATACAGTATGGAGTTGCAACGGAGCAGTATATTACTAATATTAAA
  GTTCTTTTAGAAGTGATAAAAGAAGTGCATAAGGCTAACTGTCAACTTCCAGAAAGTGCCTTCATCATAA
  ATGAGCTTTCTGGCATCTTTAACTTTGATGCAGAGGCAGGAAGAATGTTCATACGGCATAACGATCTGGA
  CTGTACAGAATCCTCAGATATGGTTGTTTTCAGTGATTTTCTATTTGTCTTTGATTTGCCATCCAAAATT
  AAACTAATGAAATGTGACTCATTTGTAAAACTGATGTCTGAAGTCATGGCATTTCCAGAAAAGATGAGCT
  CCCCACCATACCTTATACTGAAAGTCAGACGAAGTCACCTGGTGGAAGACACATTGCGTCAATTAAGACA
  AGTGGAAGACTTTGACCTTAGGAAGCAACTATCGGTTGGATTTATTAATGAAATTCGTCCTGAGGCTGGG
  GGAGTGAGTTCAGAGTTCTTCCACTGTATATTTGAAGAGATGACAGACCCCAAATATGAAATGTTCATAT
  ATCCTGAAAAGGGTTCCAGCATGTGGTTTCCAGTCAATCCCAAGTTTGAGAAGAGCAGCTATTTCCTATT
  TGGGATCTTATGTGGACTCTCCCTACATAACTTAAAGGTTATCAACCTTCCTTTCCCACTGGCTTTATAT
  AAAAAACTTTTGAACCAAAAGCCATCTTTGGAAGACTTAAAAGAACTTAGTCTTCCCTTGGGGAGGAATT
  TGCAAGAAGTTCTAAATTGTGAGGCTGGTGATATTGAAGAACTTCACATGTATTTTTCTATATACTGGGA
  TCAAAAAGATGTTGATTTAATTCCAGATGGGATCTCTGTGCCTGTGAATGAAACCAACAAGAGAGACTAT
  GTGTCTAAGTATGTTGATTACATTTTTAACATCTCTATAAAGACGATTTATGAAGAATTTCATAGAGGAT
  TTTACAAAGTGTGTAACTGGGATATAATTAGGCAATTCCAGCCCGAAGAACTAATGACAGCAATAATTGG
  AAATGCTACTTGTGACTGGAAACAATTTGAAAATAATTCAAAGTATAAAGATGGATATGACAAATCACAC
  CCAACTATTCTGCTGTTTTGGAAGGCTTTCCATGACTTAACTTTGGATGAAAAGAAAAAATTCCTCTTGT
  TTCTTACAGGATGTGATAGGCTGCATGTGAAAGGCCTACAGAATGAAGGAATAGTATTCCGGTGTTCTGA
  AACCTTCAGTGAAGAAGATAACCCAAGATCATTGACATGTCACAGGATGCTAGACCTACCTAAGTATTCC
  TCAATGCGAAGGATGAAGGAAGCACTTCAGGTTGCAATCAACAATAGCACGGGATTTGTCTCACAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224065 representing NM_025992
  Red=Cloning site Green=Tags(s)
  
  MYFSWAAGSRKPRRLKAGTSGIELLQAASGEHHSLLLFSNHRVYSCGDNSWGQLGQRRDQSTERPEPIQA
  LNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSL
  ALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSG
  QLALSGNKVKEQIYKPHSIGALKNLSVIYISCGYEHTAVLTEEGQVFTFGGNSSGQLQPSPRSGQRGPQL
  IEGIGGRVSQIECASYHTIAYVYTTGQVVSLGRGPSHTSNPTHQEAPAENSDITCLLAAEDLVDIEVKDI
  FAGAHANFVTTRRVSHTRSTGVSMKILPEIIRINQSLVKKWRAANKRKDREGAKREISLIFSSSACLTAS
  FLKKRDAGENNLIDVDLKLARDVFKKLTTEKWISSLITTCLEEYLLRDLPYSSPHQEALLVFLLLPECSI
  MQDPKNWKTLAFEFAKAIHKMGPQSLAFLRTCWASLEVSSLNILVQMLKKAIISQIQYGVATEQYITNIK
  VLLEVIKEVHKANCQLPESAFIINELSGIFNFDAEAGRMFIRHNDLDCTESSDMVVFSDFLFVFDLPSKI
  KLMKCDSFVKLMSEVMAFPEKMSSPPYLILKVRRSHLVEDTLRQLRQVEDFDLRKQLSVGFINEIRPEAG
  GVSSEFFHCIFEEMTDPKYEMFIYPEKGSSMWFPVNPKFEKSSYFLFGILCGLSLHNLKVINLPFPLALY
  KKLLNQKPSLEDLKELSLPLGRNLQEVLNCEAGDIEELHMYFSIYWDQKDVDLIPDGISVPVNETNKRDY
  VSKYVDYIFNISIKTIYEEFHRGFYKVCNWDIIRQFQPEELMTAIIGNATCDWKQFENNSKYKDGYDKSH
  PTILLFWKAFHDLTLDEKKKFLLFLTGCDRLHVKGLQNEGIVFRCSETFSEEDNPRSLTCHRMLDLPKYS
  SMRRMKEALQVAINNSTGFVSQE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025992
• ORF Size: 3009 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025992.2, NP_080268.1
• RefSeq Size: 5279 bp
• RefSeq ORF: 3012 bp
• Locus ID: 67138
• UniProt ID: F2Z461
• Cytogenetics: 6
• Gene Summary: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon.[UniProtKB/Swiss-Prot Function]