Sin3a (NM_001110350) Mouse Tagged ORF Clone

CAT#: MG224052

Sin3a (tGFP-tagged) - Mouse transcriptional regulator SIN3A (yeast) (Sin3a) transcript variant 2, (10ug)

Product Images

Circular map for MG224052

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sin3a
• Synonyms: AW553200; mKIAA4126; mSin3A; Sin3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224052 representing NM_001110350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGACGTTTGGATGACCAGGAATCACCAGTGTATGCAGCCCAGCAGCGAAGGATTCCTGGGAGCA
  CAGAGGCTTTTTCTCACCAGCACCGGGTCCTTGCCCCGGCCCCTCCTGTGTATGAAGCAGTGTCTGAGAC
  CATGCAGTCAGCTACAGGCATTCAGTACTCAGTGGCACCCAACTACCAGGTTTCAGCTGTGCCACAAAGT
  TCTGGCAGTCATGGGCCCGCCATAGCAGCAGTTCATAGCAGCCATCATCACCCAACAGCTGTCCAGCCTC
  ATGGAGGCCAGGTGGTCCAGAGCCATGCCCACCCAGCACCACCAGTTGCACCAGTACAGGGACAGCAGCA
  GTTTCAGAGGCTCAAGGTGGAAGACGCCCTGTCCTATCTTGACCAGGTGAAACTGCAGTTCGGTAGTCAG
  CCTCAGGTCTACAATGATTTCCTTGACATCATGAAGGAATTTAAATCTCAGAGCATTGATACTCCAGGAG
  TGATTAGCCGAGTGTCCCAGCTATTTAAAGGCCACCCTGATCTGATCATGGGCTTTAACACCTTCTTGCC
  TCCTGGCTACAAAATTGAGGTGCAGACTAATGACATGGTGAACGTGACAACACCTGGCCAAGTTCATCAG
  ATTCCCACCCATGGCATCCAGCCCCAGCCTCAGCCACCACCTCAGCATCCTTCCCAGCCTTCATCCCAGT
  CAGCTCCCACTCCTGCTCAGCCAGCTCCTCAGCCCACAGCTGCCAAAGTCAGCAAGCCTTCCCAACTACA
  AGCACATACTCCAGCCAGTCAGCAGACTCCCCCACTCCCACCATATGCATCCCCACGTTCTCCACCAGTC
  CAGCCTCACACACCAGTGACAATCTCCTTGGGGACAGCTCCATCTTTGCAAAACAATCAGCCTGTGGAGT
  TTAATCATGCCATCAACTATGTTAATAAGATCAAGAACAGATTCCAGGGCCAACCAGACATCTACAAAGC
  ATTCTTGGAGATTTTGCACACATACCAGAAAGAACAGCGGAATGCCAAGGAAGCTGGAGGAAACTACACT
  CCAGCTTTGACTGAGCAAGAGGTGTATGCCCAGGTGGCTCGACTCTTCAAAAACCAGGAAGATTTGTTGT
  CTGAATTTGGACAGTTCCTGCCAGATGCCAACAGCTCAGTGCTTTTAAGCAAAACAACTGCTGAGAAGGT
  TGATTCTGTGAGAAATGACCATGGAGGCACTGTGAAGAAGCCCCAACTGAATAACAAGCCACAGAGGCCC
  AGTCAGAATGGCTGCCAGATCCGCAGGCACTCTGGAACAGGAGCCACACCTCCAGTGAAGAAAAAACCCA
  AACTGATGAGTCTAAAAGAGTCTTCAATGGCAGATGCCAGCAAGCATGGTGTTGGAACGGAATCATTATT
  TTTTGATAAGGTTCGAAAGGCTCTTCGGAGTGCAGAGGCCTATGAAAACTTCCTTCGTTGCCTTGTTATC
  TTTAATCAGGAGGTGATCTCTCGGGCCGAGCTTGTACAGCTAGTCTCTCCTTTTCTGGGGAAATTCCCTG
  AATTGTTTAATTGGTTTAAAAACTTTTTGGGCTATAAGGAGTCTGTACATCTGGAAAGCTTTCCAAAGGA
  ACGAGCTACAGAAGGCATTGCCATGGAGATAGACTATGCCTCTTGTAAACGACTGGGCTCTAGCTACCGA
  GCCCTACCGAAAAGTTACCAGCAGCCCAAGTGCACGGGACGGACTCCTCTGTGTAAAGAGGTTTTAAATG
  ATACCTGGGTTTCCTTCCCATCTTGGTCTGAAGACTCCACTTTTGTTAGTTCCAAGAAGACTCAGTATGA
  AGAACATATTTACCGTTGTGAAGATGAACGATTTGAGCTTGATGTGGTTCTTGAGACCAATCTTGCAACA
  ATCCGGGTTTTAGAAGCAATACAGAAAAAACTTTCTCGCTTGTCTGCTGAGGAACAAGCCAAATTTCGCT
  TGGATAACACCCTTGGAGGCACGTCCGAAGTCATCCATCGAAAAGCACTCCAGAGGATATATGCTGACAA
  AGCAGCTGATATCATCGATGGCCTGAGGAAGAACCCCTCCATTGCTGTTCCGATTGTCCTTAAAAGGTTG
  AAGATGAAAGAAGAAGAGTGGCGAGAAGCTCAGAGAGGCTTCAACAAGGTCTGGCGAGAGCAAAATGAGA
  AGTACTACTTGAAGTCTCTGGATCACCAAGGCATCAACTTCAAGCAGAACGACACTAAGGTCTTGAGGTC
  TAAGAGCTTACTCAATGAGATCGAGAGCATCTATGACGAGAGGCAAGAGCAGGCTACAGAAGAGAACGCT
  GGTGTACCTGTTGGCCCGCACCTCTCTCTTGCCTATGAAGACAAACAGATACTAGAAGATGCTGCTGCTC
  TGATTATCCACCATGTGAAGAGGCAAACAGGCATTCAGAAAGAGGACAAATACAAAATCAAGCAAATCAT
  GCACCATTTCATTCCTGACCTGCTGTTTGCTCAGAGAGGCGATCTCTCAGATGTGGAAGAAGAGGAGGAG
  GAAGAAATGGATGTGGATGAAGCAACAGGAGCACCTAAGAAGCACAATGGTGTTGGGGGCAGCCCCCCTA
  AGTCCAAGTTGCTATTTAGTAACACAGCAGCTCAAAAGTTAAGAGGGATGGATGAAGTATATAACCTTTT
  CTATGTCAATAACAATTGGTATATCTTTATGCGACTGCATCAAATTCTCTGCTTGAGGCTGCTACGGATT
  TGTTCCCAAGCTGAACGGCAAATTGAAGAAGAAAACCGAGAGAGAGAATGGGAACGGGAGGTGCTAGGCA
  TAAAGCGAGACAAGAGTGATAGTCCTGCCATACAACTACGTCTCAAGGAACCTATGGATGTTGATGTAGA
  AGATTATTACCCAGCTTTCCTGGACATGGTGCGGAGCCTGCTTGATGGCAACATAGACTCATCACAGTAT
  GAAGATTCACTGAGAGAGATGTTCACCATTCATGCCTACATTGCCTTTACTATGGACAAATTAATCCAGA
  GCATCGTCAGACAGCTACAGCACATCGTCAGCGACGAGGTCTGTGTGCAGGTTACTGATCTTTACTTGGC
  AGAAAACAATAACGGAGCCACGGGAGGCCAGCTCAACAGTCAGACTTCAAGGAGCCTTCTGGAGTCAGCA
  TACCAGCGGAAGGCAGAGCAGCTTATGTCAGATGAGAACTGCTTCAAGCTAATGTTCATTCAAAGTCAAG
  GTCAAGTTCAGCTGACTGTTGAGCTCCTGGACACAGAAGAGGAGAACTCAGATGACCCCGTGGAAGCAGA
  GGTGTGGACACGTTGGTCAGACTACGTGGAGCGATATATGAGTTCTGATACTACTTCTCCTGAACTTCGA
  GAACATCTGGCACAGAAACCAGTATTTCTCCCAAGGAATTTGCGGCGTATCCGGAAGTGTCAACGTGGTC
  GAGAGCAACAGGAAAAAGAAGGGAAAGAAGGAAACAGCAAGAAGACCATGGAAAATGTAGAGAGCCTGGA
  TAAGCTGGAGTGTAGGTTCAAGCTGAACTCCTATAAGATGGTATATGTGATCAAATCGGAGGACTACATG
  TACCGGAGAACTGCTCTACTCAGAGCTCATCAGTCCCATGAGCGTGTAAGCAAGCGTCTGCATCAGCGGT
  TCCAGGCCTGGGTGGATAAATGGACCAAGGAGCATGTGCCTCGGGAAATGGCAGCAGAGACCAGCAAATG
  GCTCATGGGTGAGGGGCTCGAGGGCCTGGTACCCTGCACCACCACCTGTGATACAGAGACTCTGCACTTT
  GTGAGCATTAACAAATATCGTGTCAAATACGGCACAGTATTCAAAGCCCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224052 representing NM_001110350
  Red=Cloning site Green=Tags(s)
  
  MKRRLDDQESPVYAAQQRRIPGSTEAFSHQHRVLAPAPPVYEAVSETMQSATGIQYSVAPNYQVSAVPQS
  SGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQ
  PQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQ
  IPTHGIQPQPQPPPQHPSQPSSQSAPTPAQPAPQPTAAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPV
  QPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYT
  PALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRP
  SQNGCQIRRHSGTGATPPVKKKPKLMSLKESSMADASKHGVGTESLFFDKVRKALRSAEAYENFLRCLVI
  FNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLESFPKERATEGIAMEIDYASCKRLGSSYR
  ALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLAT
  IRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRL
  KMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENA
  GVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEE
  EEMDVDEATGAPKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRI
  CSQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQY
  EDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEVCVQVTDLYLAENNNGATGGQLNSQTSRSLLESA
  YQRKAEQLMSDENCFKLMFIQSQGQVQLTVELLDTEEENSDDPVEAEVWTRWSDYVERYMSSDTTSPELR
  EHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVESLDKLECRFKLNSYKMVYVIKSEDYM
  YRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHF
  VSINKYRVKYGTVFKAP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110350
• ORF Size: 3831 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110350.1, NP_001103820.1
• RefSeq Size: 5130 bp
• RefSeq ORF: 3834 bp
• Locus ID: 20466
• UniProt ID: Q60520
• Cytogenetics: 9 30.89 cM
• Gene Summary: Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904). Required for cortical neuron differentiation and callosal axon elongation (PubMed:27399968).[UniProtKB/Swiss-Prot Function]