Brd3 (NM_001113574) Mouse Tagged ORF Clone
CAT#: MG224008
- TrueORF®
Brd3 (tGFP-tagged) - Mouse bromodomain containing 3 (Brd3) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001113574" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Brd3 |
Synonyms | 2410084F24Rik; AW060456; Fsrg2; ORFX; RINGL3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224008 representing NM_001113574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACTACAGCGGCTGCCCCCACGGGGATCCCGGCAGTCCCGGGCCCCGTGAACCCTCCCCCACCTG AGGTCTCCAACCCCAGCAAGCCCGGGCGAAAGACTAACCAACTGCAGTACATGCAGAATGTAGTGGTGAA GACACTCTGGAAACATCAGTTTGCCTGGCCTTTCTACCAGCCTGTGGATGCAATCAAGCTGAACCTGCCT GATTATCATAAAATAATAAAAAACCCAATGGACATGGGGACTATCAAGAAGAGACTAGAAAATAATTATT ATTGGAGTGCCAGTGAGTGTATGCAGGACTTCAACACCATGTTTACAAACTGTTATATTTATAATAAGCC CACAGATGATATAGTGCTAATGGCCCAGGCCTTAGAGAAAATCTTTCTGCAGAAAGTGGCCCAGATGCCT CAGGAGGAAGTTGAACTATTGCCCCCTGCTCCAAAGGGCAAAGGCCGGAAGCCAGCTGCAGGAGCCCAAA ATGCAGGTTCCCAACAAGTGGCAGCTGTGTCTTCGGTCTCCCCAGCAACCCCCTTCCAGAACATACCCCC CACCGTGTCCCAGACACCCGTCATTGCCGCTACCCCTGTACCAACCATCACTGCAAACGTCACGTCAGTT CCAGTCCCCCCACCCGCTGCACCGCCTCCTCCTGCGACACCCATCGTCCCTGTGGTCCCTCCCACACCGC CTGTAGTCAAGAAAAAGGGCGTGAAGCGGAAAGCAGACACAACCACACCCACAACATCTGCCATCACTGC CAGCCGGAGTGAGTCTCCCCCACCACTTTCAGAGCCCAAGCAAGCCAAGGTAGTGGCCCGAAGGGAGAGC GGGGGCCGCCCCATCAAACCTCCCAAGAAGGACCTGGAAGATGGTGAGGTCCCACAGCACGCGGGTAAAA AGGGAAAACTGTCCGAGCACCTGCGGCACTGTGACAGCATCCTCCGGGAGATGCTGTCCAAGAAGCATGC TGCCTATGCGTGGCCCTTTTACAAGCCAGTGGACGCGGAGGCGCTGGAGCTGCATGACTACCATGACATC ATCAAGCACCCCATGGACCTCAGCACGGTCAAAAGGAAGATGGATAGCCGAGAGTACCCAGATGCACAGG GCTTTGCTGCTGATATCCGGTTAATGTTCTCGAATTGTTATAAGTACAACCCTCCAGACCACGAAGTGGT GGCCATGGCCAGGAAGCTCCAGGATGTGTTTGAGATGAGGTTTGCCAAGATGCCCGATGAGCCCATGGAG GCGCCTGCGCTGCCCGCTCCCACGGCCCCCATCGTGAGCAAGGGGGCTGAGAGCAGCCGCAGTAGTGAGG AGAGCTCTTCAGATTCAGGCAGCTCAGACTCAGAAGAGGAGCGAGCCACTCGGCTGGCTGAGCTGCAGGA GCAGACTGGCTGCGGAGCCTTCCAGGATCAGCTGTTGAATGTCTCCTCTGTGCAGCTGAAGGCCGTGCAC GAACAGCTGGCTGCCTTGTCTCAGGCCCCAGTGAACAAACCAAAGAAGAAGAAAGAGAAGAAGGAAAAGG AAAAGAAGAAGAAAGACAAGGACAAAGACAAAGAGAAGGAGAAGCACAAGGCCAAGTCTGAGGAGGAGAA GAAGGCCAAGGCAGCCCCAGCAGCCAAGCAGGCCCAGCAGAAAAAGGCTCCCACCAAGAAGGCCAACAGC ACAACCACAGCCAGCAGACAGCTCAAGAAGGGCGGCAAGCAGGCATCTGCGTCCTATGACTCAGAGGAAG AGGAGGAGGGCCTGCCCATGAGCTATGATGAAAAGCGACAACTTAGCCTTGACATCAACCGGCTGCCCGG CGAGAAGCTAGGGCGTGTGGTGCACATCATTCAGTCTCGGGAGCCCTCGCTTCGGGACTCAAACCCAGAC GAGATTGAGATTGACTTTGAGACCCTGAAGCCAACCACGCTGCGGGAACTGGAGAGATATGTCAAGTCTT GTTTACAAAAAAAGCAGAGGAAACCATTGTCAACAAGCGGGAAGAAGCAGGCAGCCAAATCGAAAGAGGA GCTAGCTCAGGAGAAGAAAAAGGAGCTGGAGAAGCGGCTGCAGGATGTCAGTGGACAACTGAACAGCAAA AAACCCACTAAGAAAGAGAAGTCCGGCTCAGCTCCCTCAGGAGGCCCATCAAGGCTCAGCAGTAGCAGCT CCTCTGAGTCTGCGAGCAGTAGCTCCAGTGGATCGAGCTCCGACAGCAGTGACTCAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224008 representing NM_001113574
Red=Cloning site Green=Tags(s) MSTTAAAPTGIPAVPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLP DYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMP QEEVELLPPAPKGKGRKPAAGAQNAGSQQVAAVSSVSPATPFQNIPPTVSQTPVIAATPVPTITANVTSV PVPPPAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSEPKQAKVVARRES GGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRHCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDI IKHPMDLSTVKRKMDSREYPDAQGFAADIRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPME APALPAPTAPIVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQTGCGAFQDQLLNVSSVQLKAVH EQLAALSQAPVNKPKKKKEKKEKEKKKKDKDKDKEKEKHKAKSEEEKKAKAAPAAKQAQQKKAPTKKANS TTTASRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPD EIEIDFETLKPTTLRELERYVKSCLQKKQRKPLSTSGKKQAAKSKEELAQEKKKELEKRLQDVSGQLNSK KPTKKEKSGSAPSGGPSRLSSSSSSESASSSSSGSSSDSSDSE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001113574 |
ORF Size | 2229 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001113574.1, NP_001107046.1 |
RefSeq Size | 5320 bp |
RefSeq ORF | 2232 bp |
Locus ID | 67382 |
UniProt ID | Q8K2F0 |
Cytogenetics | 2 A3 |
Gene Summary | Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:21536911). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (PubMed:21536911).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224008 | Brd3 (Myc-DDK-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 1 |
USD 680.00 |
|
MR224008L3 | Lenti ORF clone of Brd3 (Myc-DDK-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 1 |
USD 980.00 |
|
MR224008L4 | Lenti ORF clone of Brd3 (mGFP-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 1 |
USD 980.00 |
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