Asic5 (NM_021370) Mouse Tagged ORF Clone
CAT#: MG223851
- TrueORF®
Asic5 (tGFP-tagged) - Mouse amiloride-sensitive cation channel 5 intestinal (Accn5), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021370" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Asic5 |
Synonyms | Accn5; Blinac; Inac |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223851 representing NM_021370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACATACTGAGAAATCACAAGTGCACGCGGAGAAAGGACTCTTAGGAAAGATAAAACGTTACCTCT CAAAGAGACCACTGCCATCTCCAACTGACCGGAAGAAGTTCGATCAAGACTTTGCCATGTCCACTTCCTT TCATGGGATACATAATATTGCCCAGAACCAGAACAAAGTTCGAAAGGTGATCTGGCTGGCTGTGGTGCTG GGCTCTGTCTCGCTCTTGGTGTGGCAAATCTACAGTCGTTTGGTCAATTACTTCACATGGCCAACGACAA CATCTATAGAAGTTCAGTATGTGGAAAAAATCGAGTTCCCAGCTGTGACACTCTGTAACTTGAACAGATT CCAAACAGAAGCAGTATCTAGATTTGGCATCATTTTCTTCTTGTGGGACATAGTCTCCAAAGTCCTCCGC CTTCAGGAAATCAGTGCCAACAACACTGGCTCTCCAGAGACCTTAGATTTTGTTACGAATCACCAAAACT TCAGCATTACAGAGTTTGTCAAGAACAATGGTTTTTATCTCAATAACGACACTTTGGTGCACTGTGAGTT CTTTGGCAAAACATGCAGTCCAAAGGATTTCAAACACGTCTTTACTGAATATGGGAATTGCTTCACTTTT AACTACGGTGAGAATATCCAAAACAAGAATAAAGTGAGTGTGTCCGGAAGAGGCTTAAAGCTGCTCCTCG ACGTCCATCAGGAGGAATTCACTGACAACCCAGTCCCTGGTTTTGCTGATGCTGGTGTCATCTTTGTTAT TCACTCACCAAAGAAGGAGCCACAGTTTGATGGCTTAGGTTTGTCTTCTCCTGTGGGAATGCATGCACGG GTGACCATCCGCCAACTGAAGACGGTCCACCAGGAGTACCCATGGGGAGAATGCAATCCCAACATCAAGC TGAGAAACTTCATTACCTACAGCACTTACGGGTGTCTGAAGGAGTGCAAAGCCCGGCATATACAAAGGCT GTGTGGATGTCTGCCCTTTCTGCTTCCAGGAAATGGTGTAGAGTGTGACCTACTAGAATACTACAACTGT GTGTCTCCCATCCTTGATCACATCGAGCGTAAGGGACTATGCACGATGGGAACACACAACTCCAGCTGCC CTGTGTCATGTGAGGAAACAGAATACCCAGCTACTGTTTCTTATTCTACTTTCCCAAGTCAAAGAGCAAC AAGGTTTCTTGCCAAGAAATTGAATCAAAGCCAAGAATATATCCGGGAGAATCTCGTGAACATTGAAATA AACTATAGCGACTTAAACTATAAGATAACGCAGCAACAGAAGGCCGTGAGTGTGCCTGAGTTACTTGCAG ATGTTGGCGGTCAGCTGGGACTGTTCTGTGGAGCTAGTTTGATTACAATTATAGAAATTATTGAATATTT TTTCACCAATTTCTACTGGGTACTCATTTTCTTTTTGCTGAAAATACTTGAAACGATACAGCGGACTTCT CCACCTCAGGCAGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223851 representing NM_021370
Red=Cloning site Green=Tags(s) MEHTEKSQVHAEKGLLGKIKRYLSKRPLPSPTDRKKFDQDFAMSTSFHGIHNIAQNQNKVRKVIWLAVVL GSVSLLVWQIYSRLVNYFTWPTTTSIEVQYVEKIEFPAVTLCNLNRFQTEAVSRFGIIFFLWDIVSKVLR LQEISANNTGSPETLDFVTNHQNFSITEFVKNNGFYLNNDTLVHCEFFGKTCSPKDFKHVFTEYGNCFTF NYGENIQNKNKVSVSGRGLKLLLDVHQEEFTDNPVPGFADAGVIFVIHSPKKEPQFDGLGLSSPVGMHAR VTIRQLKTVHQEYPWGECNPNIKLRNFITYSTYGCLKECKARHIQRLCGCLPFLLPGNGVECDLLEYYNC VSPILDHIERKGLCTMGTHNSSCPVSCEETEYPATVSYSTFPSQRATRFLAKKLNQSQEYIRENLVNIEI NYSDLNYKITQQQKAVSVPELLADVGGQLGLFCGASLITIIEIIEYFFTNFYWVLIFFLLKILETIQRTS PPQAV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021370 |
ORF Size | 1485 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021370.2, NP_067345.1 |
RefSeq Size | 1657 bp |
RefSeq ORF | 1488 bp |
Locus ID | 58170 |
UniProt ID | Q9R0Y1 |
Cytogenetics | 3 E3 |
Gene Summary | Cation channel that gives rise to very low constitutive currents in the absence of activation. The activated channel exhibits selectivity for sodium and lithium, and is inhibited by amiloride (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217002 | Asic5 (untagged) - Mouse amiloride-sensitive cation channel 5, intestinal (Accn5), (10ug) |
USD 503.00 |
|
MR223851 | Asic5 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 5, intestinal (Accn5) |
USD 457.00 |
|
MR223851L3 | Lenti ORF clone of Asic5 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 5, intestinal (Accn5) |
USD 757.00 |
|
MR223851L4 | Lenti ORF clone of Asic5 (mGFP-tagged) - Mouse amiloride-sensitive cation channel 5, intestinal (Accn5) |
USD 757.00 |
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