Fbxw8 (NM_172721) Mouse Tagged ORF Clone

CAT#: MG223717

Fbxw8 (tGFP-tagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8), (10ug)

Product Images

Circular map for MG223717

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fbxw8
• Synonyms: 4930438M06Rik; FBW6; FBW8; Fbx29; FBXO29
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223717 representing NM_172721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCACAACCTGGAGGAGTTCCGCCGGCACTGGCAGGAGGAGTTGGCGCAGTCACAGGCGCTGA
  GGCGACGGCGGCGGCTCGAGGCTGGGGAGCGGCGGTCGCCGCGAAGGCCCGAGGCGGGAGCGCGAGGCGA
  GCCGGCCTCGGGCTACCTGGGGCTGGCGCAGGGGCTGCTGGAGGGCGCGGGCCGACCCCCGGCGCCCAGG
  CCTGGCCGGGGAGGCGACAGGAAGGATACGTCCAGCCGATCGCGCTCACCCCCAGACCGCGACGCCACGG
  AGCCCGAGCCGCTGGTGGATCAGCTCATCCGCGACTTGAATGAGCTGGATGACGTGCCCTTCTTTGATGT
  CCGTCTGCCTTACGAACTGGCCATCAATATATTCCAGTATCTGAACAGAAGAGAGCTGGGACTGTGTGCT
  CAGGTGAGCAAGACATGGAAGGTGATTGCTGAAGACGAGGTGCTGTGGTACCGGCTGTGCCGACAGGAGG
  GGCACCTTCCCCACAGCAGATCCTCCGATTACACCTGCTGGAAGCTCATCTTGCAAGAGTGCCTCGCCAA
  GGAGCACACATTAAGAGCCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGGAGCATGTTCCCGAC
  GCTGTTTTGTGTGACGTGCGTTCTCACGATGGTGTGGTCATTGCTGGATACACGTCAGGGGATGTGCGGG
  TGTGGGACACCCGCACCTGGGACTACGTGGCCCCCTTCCTGGAGTCTGAGTCTGAGGAGGAGGATCCTGG
  AATGCAGCCATATGTCTCCTTTGTGAGGATCAACAGCTCGCTGGCAGTGGCGGCTTACGAGGATGGGATC
  CTTAACATTTGGGACCTGAGAACCGGAAGGTTCCCTATCTTTCGTTTTGAGCATGACGCAAGAATACAAG
  CCCTTGCGCTGAGCCAAGAAAAGCCCATTGTTGCCACGGCTTCTGCTTTTGACGTTGTGATGTTGTACCC
  CAACGAGGAGGGGCATTGGCATGTGGCCTCGGAGTTTGAAGTTCAGAAGCTGGTTGACTACCTTGAAATA
  GTTCCGAATACTGGGAGGTACCCTGTGGCAATAGCCACAGCCGGGGATCTGGTGTACCTGCTGAAGGCCG
  ACGACTCAGCCAGAACCCTTCATTATGTCTATGGCCAGCCTGCCACATGTCTGGATGTCTCAGCCAGCCA
  GGTTGCCTTTGGAGTGAAGAGTCTGGGATGGGTGTATGAAGGAAACAAGATCCTGGTGTACAGCCTGGAA
  GCAGAGCGCTGCCTCTCGAAGCTGGGCAATGCACTTGGAGACTTTACCTGTGTCAACATCCGGGATAGCC
  CTCCCAACCTCATGGTCAGCGGCAACATGGACAGGAGAGTGAGGATCCATGACCTCCGCAGCGATAAGAT
  CGCCCTGTCGCTGTCTGCCCATCAGCTGGGGGTGTCCGCAGTCCAGATGGATGACTGGAAGGTTGTCAGT
  GGAGGCGAGGAGGGGCTGGTGTCTGTGTGGGATTACCGCATGAACCAGAAGCTGTGGGAAGTGCACTCCA
  GGCACCCTGTGCGCTATCTCTCCTTCAATAGCCACAGCCTCATCACTGCCAACGTGCCCTACGAGAAGGT
  GCTGCGAAACTCCGACCTCGACAACTTTGCCTGTCACAGGAGACATCGTGGCCTGATCCATGCCTATGAA
  TTTGCTGTGGACCAGCTGGCCTTTCAGAGCCCCCTTCCTGTCTGCCGCTTACCCCGTGACATCATGGCTG
  GATACAGCTATGACCTCGCACTGTCTTTCCCCCATGACAGTATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223717 representing NM_172721
  Red=Cloning site Green=Tags(s)
  
  MDDHNLEEFRRHWQEELAQSQALRRRRRLEAGERRSPRRPEAGARGEPASGYLGLAQGLLEGAGRPPAPR
  PGRGGDRKDTSSRSRSPPDRDATEPEPLVDQLIRDLNELDDVPFFDVRLPYELAINIFQYLNRRELGLCA
  QVSKTWKVIAEDEVLWYRLCRQEGHLPHSRSSDYTCWKLILQECLAKEHTLRANWKNRKGAVSELEHVPD
  AVLCDVRSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESESEEEDPGMQPYVSFVRINSSLAVAAYEDGI
  LNIWDLRTGRFPIFRFEHDARIQALALSQEKPIVATASAFDVVMLYPNEEGHWHVASEFEVQKLVDYLEI
  VPNTGRYPVAIATAGDLVYLLKADDSARTLHYVYGQPATCLDVSASQVAFGVKSLGWVYEGNKILVYSLE
  AERCLSKLGNALGDFTCVNIRDSPPNLMVSGNMDRRVRIHDLRSDKIALSLSAHQLGVSAVQMDDWKVVS
  GGEEGLVSVWDYRMNQKLWEVHSRHPVRYLSFNSHSLITANVPYEKVLRNSDLDNFACHRRHRGLIHAYE
  FAVDQLAFQSPLPVCRLPRDIMAGYSYDLALSFPHDSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172721
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172721.2, NP_766309.2
• RefSeq Size: 4978 bp
• RefSeq ORF: 1797 bp
• Locus ID: 231672
• UniProt ID: Q8BIA4
• Cytogenetics: 5 F
• Gene Summary: Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (By similarity).[UniProtKB/Swiss-Prot Function]