Fbxw8 (NM_172721) Mouse Tagged ORF Clone
CAT#: MG223717
- TrueORF®
Fbxw8 (tGFP-tagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_172721" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Fbxw8 |
Synonyms | 4930438M06Rik; FBW6; FBW8; Fbx29; FBXO29 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223717 representing NM_172721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACCACAACCTGGAGGAGTTCCGCCGGCACTGGCAGGAGGAGTTGGCGCAGTCACAGGCGCTGA GGCGACGGCGGCGGCTCGAGGCTGGGGAGCGGCGGTCGCCGCGAAGGCCCGAGGCGGGAGCGCGAGGCGA GCCGGCCTCGGGCTACCTGGGGCTGGCGCAGGGGCTGCTGGAGGGCGCGGGCCGACCCCCGGCGCCCAGG CCTGGCCGGGGAGGCGACAGGAAGGATACGTCCAGCCGATCGCGCTCACCCCCAGACCGCGACGCCACGG AGCCCGAGCCGCTGGTGGATCAGCTCATCCGCGACTTGAATGAGCTGGATGACGTGCCCTTCTTTGATGT CCGTCTGCCTTACGAACTGGCCATCAATATATTCCAGTATCTGAACAGAAGAGAGCTGGGACTGTGTGCT CAGGTGAGCAAGACATGGAAGGTGATTGCTGAAGACGAGGTGCTGTGGTACCGGCTGTGCCGACAGGAGG GGCACCTTCCCCACAGCAGATCCTCCGATTACACCTGCTGGAAGCTCATCTTGCAAGAGTGCCTCGCCAA GGAGCACACATTAAGAGCCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGGAGCATGTTCCCGAC GCTGTTTTGTGTGACGTGCGTTCTCACGATGGTGTGGTCATTGCTGGATACACGTCAGGGGATGTGCGGG TGTGGGACACCCGCACCTGGGACTACGTGGCCCCCTTCCTGGAGTCTGAGTCTGAGGAGGAGGATCCTGG AATGCAGCCATATGTCTCCTTTGTGAGGATCAACAGCTCGCTGGCAGTGGCGGCTTACGAGGATGGGATC CTTAACATTTGGGACCTGAGAACCGGAAGGTTCCCTATCTTTCGTTTTGAGCATGACGCAAGAATACAAG CCCTTGCGCTGAGCCAAGAAAAGCCCATTGTTGCCACGGCTTCTGCTTTTGACGTTGTGATGTTGTACCC CAACGAGGAGGGGCATTGGCATGTGGCCTCGGAGTTTGAAGTTCAGAAGCTGGTTGACTACCTTGAAATA GTTCCGAATACTGGGAGGTACCCTGTGGCAATAGCCACAGCCGGGGATCTGGTGTACCTGCTGAAGGCCG ACGACTCAGCCAGAACCCTTCATTATGTCTATGGCCAGCCTGCCACATGTCTGGATGTCTCAGCCAGCCA GGTTGCCTTTGGAGTGAAGAGTCTGGGATGGGTGTATGAAGGAAACAAGATCCTGGTGTACAGCCTGGAA GCAGAGCGCTGCCTCTCGAAGCTGGGCAATGCACTTGGAGACTTTACCTGTGTCAACATCCGGGATAGCC CTCCCAACCTCATGGTCAGCGGCAACATGGACAGGAGAGTGAGGATCCATGACCTCCGCAGCGATAAGAT CGCCCTGTCGCTGTCTGCCCATCAGCTGGGGGTGTCCGCAGTCCAGATGGATGACTGGAAGGTTGTCAGT GGAGGCGAGGAGGGGCTGGTGTCTGTGTGGGATTACCGCATGAACCAGAAGCTGTGGGAAGTGCACTCCA GGCACCCTGTGCGCTATCTCTCCTTCAATAGCCACAGCCTCATCACTGCCAACGTGCCCTACGAGAAGGT GCTGCGAAACTCCGACCTCGACAACTTTGCCTGTCACAGGAGACATCGTGGCCTGATCCATGCCTATGAA TTTGCTGTGGACCAGCTGGCCTTTCAGAGCCCCCTTCCTGTCTGCCGCTTACCCCGTGACATCATGGCTG GATACAGCTATGACCTCGCACTGTCTTTCCCCCATGACAGTATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223717 representing NM_172721
Red=Cloning site Green=Tags(s) MDDHNLEEFRRHWQEELAQSQALRRRRRLEAGERRSPRRPEAGARGEPASGYLGLAQGLLEGAGRPPAPR PGRGGDRKDTSSRSRSPPDRDATEPEPLVDQLIRDLNELDDVPFFDVRLPYELAINIFQYLNRRELGLCA QVSKTWKVIAEDEVLWYRLCRQEGHLPHSRSSDYTCWKLILQECLAKEHTLRANWKNRKGAVSELEHVPD AVLCDVRSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESESEEEDPGMQPYVSFVRINSSLAVAAYEDGI LNIWDLRTGRFPIFRFEHDARIQALALSQEKPIVATASAFDVVMLYPNEEGHWHVASEFEVQKLVDYLEI VPNTGRYPVAIATAGDLVYLLKADDSARTLHYVYGQPATCLDVSASQVAFGVKSLGWVYEGNKILVYSLE AERCLSKLGNALGDFTCVNIRDSPPNLMVSGNMDRRVRIHDLRSDKIALSLSAHQLGVSAVQMDDWKVVS GGEEGLVSVWDYRMNQKLWEVHSRHPVRYLSFNSHSLITANVPYEKVLRNSDLDNFACHRRHRGLIHAYE FAVDQLAFQSPLPVCRLPRDIMAGYSYDLALSFPHDSI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_172721 |
ORF Size | 1794 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172721.2, NP_766309.2 |
RefSeq Size | 4978 bp |
RefSeq ORF | 1797 bp |
Locus ID | 231672 |
UniProt ID | Q8BIA4 |
Cytogenetics | 5 F |
Gene Summary | Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219576 | Fbxw8 (untagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8), (10ug) |
USD 613.00 |
|
MR223717 | Fbxw8 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8) |
USD 558.00 |
|
MR223717L3 | Lenti ORF clone of Fbxw8 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8) |
USD 858.00 |
|
MR223717L4 | Lenti ORF clone of Fbxw8 (mGFP-tagged) - Mouse F-box and WD-40 domain protein 8 (Fbxw8) |
USD 858.00 |
{0} Product Review(s)
Be the first one to submit a review