Hrh3 (NM_133849) Mouse Tagged ORF Clone
CAT#: MG223520
- TrueORF®
Hrh3 (tGFP-tagged) - Mouse histamine receptor H3 (Hrh3), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_133849" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Hrh3 |
Synonyms | AW049250; H3R; HH3R |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223520 representing NM_133849
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGCGCCGCCCGACGGGCTGATGAACGCGTCGGGCGCTCTGGCCGGAGAGGCGGCGGCTGCAG GCGGGGCGCGCGGCTTCTCGGCTGCCTGGACCGCTGTCCTGGCTGCGCTCATGGCGCTGCTCATCGTGGC CACAGTGCTGGGCAACGCGCTGGTCATGCTCGCCTTCGTGGCGGATTCGAGCCTCCGCACCCAGAACAAC TTCTTTCTGCTCAACCTCGCCATCTCCGACTTCCTCGTGGGTGCCTTCTGCATCCCATTGTATGTACCCT ATGTGCTGACCGGCCGTTGGACCTTTGGCCGGGGCCTCTGCAAGCTGTGGCTGGTGGTAGACTACCTACT GTGTGCCTCCTCAGTCTTCAACATCGTGCTGATCAGCTATGACCGATTCCTGTCAGTCACTCGAGCTGTC TCCTACCGGGCCCAGCAGGGGGACACAAGACGGGCTGTTCGGAAGATGGCACTGGTGTGGGTGCTGGCCT TCCTGCTGTATGGGCCTGCCATCCTGAGTTGGGAGTACCTGTCCGGTGGCAGCTCCATCCCCGAGGGCCA CTGCTATGCTGAGTTCTTCTACAACTGGTACTTTCTCATCACGGCCTCCACCCTCGAGTTCTTCACACCC TTCCTCAGCGTTACCTTCTTCAACCTCAGCATCTACCTGAACATCCAGAGGCGCACTCGTCTTCGGCTGG ATGGGGGCCGAGAGGCTGGTCCAGAACCCCCACCTGATGCCCAACCCTCGCCACCTCCAGCTCCCCCCAG CTGCTGGGGCTGCTGGCCAAAGGGGCACGGGGAGGCCATGCCATTGCACAGGTATGGGGTGGGTGAGGCA GGCCCTGGTGTTGAGACTGGGGAGGCTGGCCTCGGGGGTGGCAGCGGTGGAGGCGCTGCTGCCTCGCCTA CCTCCAGCTCCGGCAGCTCCTCAAGGGGCACTGAGAGGCCACGCTCACTCAAAAGGGGCTCCAAGCCATC AGCGTCTTCAGCGTCCTTGGAGAAGCGCATGAAGATGGTATCCCAAAGCATCACCCAGCGCTTTCGGCTG TCGCGGGACAAGAAGGTAGCCAAGTCGCTGGCTATCATCGTGAGCATCTTTGGGCTCTGCTGGGCCCCGT ACACACTCCTCATGATCATCCGGGCTGCTTGCCATGGCCACTGCGTCCCCGACTACTGGTACGAGACGTC CTTCTGGCTTCTGTGGGCCAACTCGGCCGTCAACCCCGTCCTCTACCCACTGTGCCACTACAGCTTCCGT AGAGCCTTCACCAAGCTCCTCTGCCCCCAGAAGCTCAAGGTCCAGCCCCATGGCTCCCTGGAGCAGTGCT GGAAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223520 representing NM_133849
Red=Cloning site Green=Tags(s) MERAPPDGLMNASGALAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNN FFLLNLAISDFLVGAFCIPLYVPYVLTGRWTFGRGLCKLWLVVDYLLCASSVFNIVLISYDRFLSVTRAV SYRAQQGDTRRAVRKMALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTP FLSVTFFNLSIYLNIQRRTRLRLDGGREAGPEPPPDAQPSPPPAPPSCWGCWPKGHGEAMPLHRYGVGEA GPGVETGEAGLGGGSGGGAAASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSITQRFRL SRDKKVAKSLAIIVSIFGLCWAPYTLLMIIRAACHGHCVPDYWYETSFWLLWANSAVNPVLYPLCHYSFR RAFTKLLCPQKLKVQPHGSLEQCWK SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133849 |
ORF Size | 2638 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133849.3, NP_598610.1 |
RefSeq ORF | 1338 bp |
Locus ID | 99296 |
UniProt ID | P58406 |
Cytogenetics | 2 102.62 cM |
Gene Summary | The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216026 | Hrh3 (untagged) - Mouse histamine receptor H3 (Hrh3), (10ug) |
USD 503.00 |
|
MR223520 | Hrh3 (Myc-DDK-tagged) - Mouse histamine receptor H3 (Hrh3) |
USD 457.00 |
|
MR223520L3 | Lenti ORF clone of Hrh3 (Myc-DDK-tagged) - Mouse histamine receptor H3 (Hrh3) |
USD 757.00 |
|
MR223520L4 | Lenti ORF clone of Hrh3 (mGFP-tagged) - Mouse histamine receptor H3 (Hrh3) |
USD 757.00 |
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