Notch4 (NM_010929) Mouse Tagged ORF Clone

CAT#: MG223369

Notch4 (tGFP-tagged) - Mouse Notch gene homolog 4 (Drosophila) (Notch4), (10ug)

Product Images

Circular map for MG223369

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Notch4
• Synonyms: Int-3; Int3; N4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223369 representing NM_010929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCCAGTTGCTGCTGCTGCTGCTCTTGCCACTCAATTTCCCTGTCATCCTGACCAGAGAGCTTC
  TGTGTGGAGGATCCCCAGAGCCCTGTGCCAACGGAGGCACCTGCCTGAGGCTATCTCGGGGACAAGGGAT
  CTGCCAGTGTGCCCCTGGATTTCTGGGTGAGACTTGCCAGTTTCCTGACCCCTGCAGGGATACCCAACTC
  TGCAAGAATGGTGGCAGCTGCCAAGCCCTGCTCCCCACACCCCCAAGCTCCCGTAGTCCTACTTCTCCAC
  TGACCCCTCACTTCTCCTGCACCTGCCCCTCTGGCTTCACCGGTGATCGATGCCAAACCCATCTGGAAGA
  GCTCTGTCCACCTTCTTTCTGTTCCAACGGGGGTCACTGCTATGTTCAGGCCTCAGGCCGCCCACAGTGC
  TCCTGCGAGCCTGGGTGGACAGGTGAGCAATGCCAGCTCCGAGACTTCTGCTCAGCCAACCCCTGTGCCA
  ACGGAGGCGTGTGCCTGGCCACATACCCCCAGATCCAGTGCCGCTGTCCACCTGGGTTCGAGGGTCACAC
  CTGTGAACGCGACATCAACGAGTGCTTCCTGGAGCCGGGACCCTGCCCTCAGGGCACCTCCTGCCATAAC
  ACCTTGGGTTCCTACCAGTGTCTCTGCCCTGTGGGGCAGGAAGGTCCCCAGTGCAAGCTCAGGAAGGGAG
  CCTGCCCTCCTGGAAGCTGTCTCAATGGGGGCACCTGCCAGCTGGTCCCAGAGGGACACTCCACCTTTCA
  TCTCTGCCTCTGTCCCCCAGGTTTCACGGGGCTGGACTGTGAGATGAACCCAGATGACTGTGTCAGGCAC
  CAGTGTCAGAACGGGGCCACCTGTCTGGATGGGCTGGACACCTACACCTGCCTCTGCCCCAAGACATGGA
  AGGGCTGGGACTGCTCTGAAGATATAGATGAATGTGAAGCCCGGGGTCCCCCTCGCTGCAGGAACGGTGG
  CACCTGCCAGAACACAGCTGGCAGCTTTCACTGTGTGTGCGTGAGTGGCTGGGGAGGCGCAGGCTGTGAG
  GAGAACCTGGATGACTGTGCAGCTGCCACCTGTGCCCCGGGATCCACCTGCATCGACCGTGTGGGCTCCT
  TCTCCTGCCTCTGCCCACCTGGACGCACAGGCCTCCTGTGCCACCTGGAAGACATGTGTTTGAGTCAGCC
  GTGCCACGTGAATGCCCAGTGCAGCACCAACCCTCTGACAGGCTCCACCCTCTGCATATGCCAGCCTGGC
  TACTCAGGATCCACCTGTCACCAAGATCTGGATGAGTGCCAAATGGCCCAGCAAGGACCCAGTCCCTGCG
  AACATGGCGGCTCCTGCATCAACACCCCTGGCTCCTTCAACTGCCTCTGCCTGCCTGGTTACACGGGCTC
  CCGCTGTGAAGCTGACCACAATGAGTGCCTGTCACAGCCCTGCCACCCAGGCAGCACCTGCCTGGACCTG
  CTTGCAACCTTCCACTGCCTCTGCCCACCAGGCTTGGAAGGGAGGCTCTGTGAGGTGGAGGTCAATGAGT
  GCACCTCTAATCCCTGCCTGAACCAAGCTGCCTGCCATGACCTGCTCAACGGCTTCCAGTGCCTCTGCCT
  TCCTGGATTCACCGGCGCCCGATGTGAGAAAGACATGGACGAGTGTAGCAGCACCCCCTGTGCCAATGGG
  GGGCGCTGCCGAGACCAGCCTGGAGCCTTCTACTGCGAGTGTCTCCCAGGCTTTGAAGGGCCACACTGTG
  AGAAAGAAGTGGACGAATGTCTGAGTGACCCCTGCCCCGTGGGAGCCAGCTGCCTTGATCTCCCCGGAGC
  ATTCTTCTGCCTCTGCCGTCCTGGTTTCACAGGTCAACTTTGTGAGGTTCCCTTGTGCACCCCCAACATG
  TGCCAACCTGGACAGCAATGCCAAGGTCAGGAACACAGAGCCCCCTGCCTCTGCCCTGACGGAAGTCCTG
  GCTGTGTTCCTGCCGAGGACAACTGCCCCTGTCACCATGGCCATTGCCAGAGATCCTTGTGTGTGTGTGA
  TGAGGGCTGGACTGGACCAGAATGCGAGACAGAACTGGGTGGCTGCATCTCCACACCCTGTGCCCATGGG
  GGGACCTGCCACCCACAGCCATCTGGCTACAACTGTACCTGCCCTGCAGGCTACATGGGGTTGACCTGTA
  GTGAGGAGGTGACAGCTTGTCACTCAGGGCCCTGTCTCAATGGTGGCTCCTGCAGCATCCGTCCTGAGGG
  CTATTCCTGCACCTGCCTTCCAAGTCACACAGGTCGCCACTGCCAGACTGCCGTGGACCACTGTGTGTCT
  GCCTCGTGCCTCAATGGGGGTACCTGTGTGAACAAGCCTGGCACTTTCTTCTGCCTCTGTGCCACTGGCT
  TCCAGGGGCTGCACTGTGAGGAGAAGACTAACCCCAGCTGTGCAGACAGCCCCTGCAGGAACAAGGCAAC
  CTGCCAAGACACACCTCGAGGGGCCCGCTGCCTCTGCAGCCCTGGCTATACAGGAAGCAGCTGCCAGACT
  CTGATAGACTTGTGTGCCCGGAAGCCCTGTCCACACACTGCTCGATGCCTCCAGAGTGGGCCCTCGTTCC
  AGTGCCTGTGCCTCCAGGGATGGACAGGGGCTCTCTGTGACTTCCCACTGTCCTGCCAGAAGGCCGCGAT
  GAGCCAAGGCATAGAGATCTCTGGCCTGTGCCAGAATGGAGGCCTCTGTATTGACACGGGCTCCTCCTAT
  TTCTGCCGCTGCCCTCCTGGATTCCAAGGCAAGTTATGCCAGGATAATGTGAACCCCTGTGAGCCCAATC
  CCTGCCATCACGGGTCTACCTGTGTGCCTCAGCCCAGTGGCTATGTCTGCCAGTGTGCCCCAGGCTATGA
  GGGACAGAACTGCTCAAAAGTACTTGACGCTTGTCAGTCCCAGCCCTGCCACAACCACGGAACCTGTACC
  TCCAGGCCTGGAGGCTTCCACTGTGCCTGCCCTCCAGGCTTCGTGGGACTGCGCTGTGAGGGAGATGTGG
  ATGAGTGTCTGGACCGGCCCTGTCACCCCTCGGGCACTGCAGCTTGCCACTCTTTAGCCAACGCCTTCTA
  CTGCCAGTGTCTGCCTGGGCACACAGGCCAGCGGTGTGAGGTGGAGATGGACCTCTGTCAGAGCCAACCC
  TGCTCCAATGGAGGATCCTGTGAGATCACAACAGGGCCACCCCCTGGCTTCACCTGTCACTGCCCCAAGG
  GTTTTGAAGGCCCCACCTGCAGCCACAAAGCCCTTTCCTGCGGCATCCATCACTGCCACAATGGAGGCCT
  ATGTCTGCCCTCCCCTAAGCCAGGGTCACCACCACTCTGTGCCTGCCTCAGTGGTTTTGGGGGCCCTGAC
  TGTCTGACACCTCCAGCTCCACCGGGCTGCGGTCCCCCCTCACCCTGCCTGCACAATGGTACCTGCACTG
  AGACCCCTGGGTTGGGCAACCCGGGCTTTCAATGCACCTGCCCTCCTGACTCTCCAGGGCCCCGGTGTCA
  AAGGCCAGGGGCAAGTGGGTGTGAGGGCCGAGGTGGTGATGGGACCTGCGATGCTGGCTGCAGTGGCCCA
  GGAGGAGACTGGGATGGAGGGGACTGTTCCCTGGGGGTCCCAGACCCCTGGAAGGGCTGTCCCCCGCATT
  CCCAGTGCTGGCTTCTGTTCCGGGACGGACGGTGTCACCCGCAGTGTGACTCTGAGGAGTGTCTCTTTGA
  TGGCTACGACTGTGAAATCCCTCTAACCTGCATCCCAGCCTATGACCAGTACTGCCGAGATCACTTCCAC
  AACGGGCACTGTGAGAAAGGCTGCAATAACGCTGAATGTGGCTGGGACGGGGGAGACTGCAGACCAGAAG
  GGGAAGACTCAGAGGGGAGGCCCTCCCTGGCCCTGCTGGTGGTGCTGAGGCCCCCAGCCCTGGATCAGCA
  GCTGCTTGCCCTGGCACGAGTGCTGTCCCTGACTCTGAGGGTCGGTCTCTGGGTGAGGAAGGACAGTGAA
  GGCAGGAACATGGTGTTCCCCTATCCTGGGACCCGGGCCAAAGAGGAGCTGAGTGGAGCTAGGGATTCCT
  CTTCATGGGAAAGACAAGCCCCTCCCACTCAGCCCCTGGGCAAGGAGACAGAGTCTCTTGGTGCAGGGTT
  TGTGGTAGTGATGGGAGTGGATCTGTCCCGCTGTGGTCCGGAACATCCTGCGTCCCGCTGCCCCTGGGAC
  TCTGGACTCCTGCTGCGCTTCCTTGCAGCAATGGCAGCAGTGGGAGCTCTGGAGCCCCTGCTGCCTGGAC
  CCTTGCTGGCGGCTCACCCTCAAGCAGGGACCAGGCCCCCTGCCAACCAGCTTCCCTGGCCCATTCTATG
  TTCACCAGTGGTTGGGGTGCTTCTCCTGGCCCTTGGGGCCCTTCTCGTCCTCCAGCTCATTCGGCGCCGG
  CGACGAGAACATGGGGCCCTGTGGCTGCCCCCTGGTTTCATTCGAAGGCCTCAGGCACAGCAGGCACCCC
  ACCGGCGGAGGCCCCCACTGGGCGAGGACAACATTGGTCTTAAGGCACTGAAGCCAGAGGCCGAAGTGGA
  TGAGGATGGAGTGGCCATGTGCTCGGGCCCTGAAGAGGGAGAGGCTGAAGAAACAGCCTCAGCCTCCAGG
  TGCCAGCTTTGGCCGCTCAACAGCAGCTGTGGAGAGCTCCCCCAGGCAGCCATGCTGACCCCTCCTCAGG
  AGTGTGAATCGGAGGTTCTGGATGTGGACACCTGTGGACCTGATGGGGTGACACCCCTGATGTCAGCCGT
  CTTCTGTGGGGGAGTGCAGTCCACGACTGGGGCTAGTCCACAGAGACTGGGGCTAGGAAATCTGGAACCC
  TGGGAACCACTGCTGGATAGAGGGGCCTGCCCCCAGGCTCACACTGTGGGCACTGGAGAGACGCCTCTGC
  ACCTAGCTGCCAGATTCTCTCGGCCAACCGCTGCCCGTCGCCTTCTTGAGGCTGGAGCCAACCCCAATCA
  GCCAGACCGCGCTGGGCGCACCCCACTTCACACTGCTGTGGCTGCCGACGCTCGGGAGGTTTGCCAGCTC
  CTATTGGCCAGCAGACAGACTTCGGTGGACGCCCGCACAGAGGACGGGACTACACCTTTGATGCTGGCTG
  CCAGGCTGGCCGTGGAGGACCTGGTTGAAGAATTGATCGCAGCCCGAGCAGATGTAGGAGCCAGGGATAA
  AAGGGGAAAAACTGCACTGCACTGGGCCGCTGCTGTGAACAACGCCCGAGCCGCCCGCTCTCTCCTCCAG
  GCTGGAGCGGATAAAGATGCCCAGGACAGTAGGGAACAGACGCCGCTTTTCCTGGCAGCGCGCGAAGGAG
  CCGTGGAGGTGGCGCAGCTGTTGCTGGAGCTCGGGGCGGCCCGGGGACTGCGAGACCAGGCCGGGCTGGC
  CCCAGGAGATGTGGCCCGCCAGCGCAGTCACTGGGACCTGCTAACGCTGCTGGAAGGGGCTGGACCGACT
  ACGCAGGAGGCCCGTGCGCACGCACGCACCACGCCGGGGGGCGGGGCCGCGCCGCGCTGCCGGACGCTGT
  CTGCGGGAGCGCGCCCGCGCGGGGGCGGAGCCTGTCTGCAGGCTCGCACTTGGTCGGTGGACTTGGGAGC
  GCGCGGAGGGAAGGTGTATGCTCGCTGCCGGAGCCGATCTGGAAGCTGCGGAGGCCCCACCACGCGCGGC
  CGCAGGTTCTCCGCGGGCTCCCGTGGACGACGCGGGGCTAGGGCATCACAGGATGACTGGCCTCGCGACT
  GGGTGGCCCTGGAAGCCTGCGGCTCCGCCTGCAGTGCGCCGATCCCGCCTCCCAGCCTGACCCCGTCCCC
  AGAACGTGGATCCCCTCAAGTTGCCTGGGGTCTTCCAGTTCACCAAGAGATTCCCTTAAACTCGGTTGTA
  AGAAATCTGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223369 representing NM_010929
  Red=Cloning site Green=Tags(s)
  
  MQPQLLLLLLLPLNFPVILTRELLCGGSPEPCANGGTCLRLSRGQGICQCAPGFLGETCQFPDPCRDTQL
  CKNGGSCQALLPTPPSSRSPTSPLTPHFSCTCPSGFTGDRCQTHLEELCPPSFCSNGGHCYVQASGRPQC
  SCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPPGFEGHTCERDINECFLEPGPCPQGTSCHN
  TLGSYQCLCPVGQEGPQCKLRKGACPPGSCLNGGTCQLVPEGHSTFHLCLCPPGFTGLDCEMNPDDCVRH
  QCQNGATCLDGLDTYTCLCPKTWKGWDCSEDIDECEARGPPRCRNGGTCQNTAGSFHCVCVSGWGGAGCE
  ENLDDCAAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHVNAQCSTNPLTGSTLCICQPG
  YSGSTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDL
  LATFHCLCPPGLEGRLCEVEVNECTSNPCLNQAACHDLLNGFQCLCLPGFTGARCEKDMDECSSTPCANG
  GRCRDQPGAFYCECLPGFEGPHCEKEVDECLSDPCPVGASCLDLPGAFFCLCRPGFTGQLCEVPLCTPNM
  CQPGQQCQGQEHRAPCLCPDGSPGCVPAEDNCPCHHGHCQRSLCVCDEGWTGPECETELGGCISTPCAHG
  GTCHPQPSGYNCTCPAGYMGLTCSEEVTACHSGPCLNGGSCSIRPEGYSCTCLPSHTGRHCQTAVDHCVS
  ASCLNGGTCVNKPGTFFCLCATGFQGLHCEEKTNPSCADSPCRNKATCQDTPRGARCLCSPGYTGSSCQT
  LIDLCARKPCPHTARCLQSGPSFQCLCLQGWTGALCDFPLSCQKAAMSQGIEISGLCQNGGLCIDTGSSY
  FCRCPPGFQGKLCQDNVNPCEPNPCHHGSTCVPQPSGYVCQCAPGYEGQNCSKVLDACQSQPCHNHGTCT
  SRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTAACHSLANAFYCQCLPGHTGQRCEVEMDLCQSQP
  CSNGGSCEITTGPPPGFTCHCPKGFEGPTCSHKALSCGIHHCHNGGLCLPSPKPGSPPLCACLSGFGGPD
  CLTPPAPPGCGPPSPCLHNGTCTETPGLGNPGFQCTCPPDSPGPRCQRPGASGCEGRGGDGTCDAGCSGP
  GGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPLTCIPAYDQYCRDHFH
  NGHCEKGCNNAECGWDGGDCRPEGEDSEGRPSLALLVVLRPPALDQQLLALARVLSLTLRVGLWVRKDSE
  GRNMVFPYPGTRAKEELSGARDSSSWERQAPPTQPLGKETESLGAGFVVVMGVDLSRCGPEHPASRCPWD
  SGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTRPPANQLPWPILCSPVVGVLLLALGALLVLQLIRRR
  RREHGALWLPPGFIRRPQAQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVAMCSGPEEGEAEETASASR
  CQLWPLNSSCGELPQAAMLTPPQECESEVLDVDTCGPDGVTPLMSAVFCGGVQSTTGASPQRLGLGNLEP
  WEPLLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQL
  LLASRQTSVDARTEDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARSLLQ
  AGADKDAQDSREQTPLFLAAREGAVEVAQLLLELGAARGLRDQAGLAPGDVARQRSHWDLLTLLEGAGPT
  TQEARAHARTTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARGGKVYARCRSRSGSCGGPTTRG
  RRFSAGSRGRRGARASQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVAWGLPVHQEIPLNSVV
  RNLN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010929
• ORF Size: 5892 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010929.2, NP_035059.2
• RefSeq Size: 6604 bp
• RefSeq ORF: 5895 bp
• Locus ID: 18132
• UniProt ID: P31695
• Cytogenetics: 17 18.15 cM
• Gene Summary: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system.[UniProtKB/Swiss-Prot Function]