Tmed8 (NM_001033475) Mouse Tagged ORF Clone
CAT#: MG223140
- TrueORF®
Tmed8 (tGFP-tagged) - Mouse transmembrane emp24 domain containing 8 (Tmed8), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001033475" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tmed8 |
Synonyms | 6430595O10Rik; AI447224; Gm1184; Mem1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223140 representing NM_001033475
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACCGGCAGGCAGCTGAGGGGCCGGCCTTCTGGAGCCCCGCAGCTCGCCGAGGGTCGGCGGGCG GCGTCGGGGACCGACGGGGAGTGGAGGAGAGCCAGGCGGCCGCCTCAGAGAAGGAAGATCTGGAAAGCAC CAACGTCAGCTCTCCACTGGCTTCTGCTTCTGATCCAGCAGCCGAGTCCTCACCATACAGGCCACAGATG GTATCTCCAGCGAGTAAGGATACCACGGAAGATCTACAGAACGTGGCTGGTGCCTCAGAGGGTCAGGCTC CGGGGGAGCAGGCGGCGCTGCCAGCAGGCCAGACCCAGGTTCTCAGTGAGATGGCCAAGTACCAAGCTCC ACAGCGACCTGAGGACACAGTTATGATCCAGTCTGAGCACACTGGGGCTATAGATGTTCTTCCAGCGGAC TTGGAATCTGCAGATCTTCTGGGGGACCACAGGAAAGTCTCTCCGCCTCTGATGGCTCCTCCGTGCGTCT GGACCTTTGCCAAAGTGAAGGAGTTCAAAAGCAAGCTGGGCAAGGAGAAGAACAGCCGTCTGGTAGTGAA GCGGGGAGAAGTGGTCACCATCCGGGTCCCTACCCACCCCGAGGGAAAGCGCGTGTGCTGGGAATTTGCA ACTGATGACTATGATATTGGTTTTGGAGTTTATTTTGACTGGACCCCAGTAACCAGCACTGATATAACCG TGCAGGTCAGTGACTCCAGTGAGGATGAGGAGGAGGAAGAGGATGAAGAGGAGGAGATTGAAGAACCCGT CCCGGTGGGAGATGTAGAGAGAGGCTCCAGGAGTTCTCTACGGGGCCGCTATGGGGAGGTCATGCCTGTG TACCGGCGGGACAGCCACCGAGATGTGCAAGCTGGCAGCCATGATTACCCTGGGGAGGGCATCTACTTGC TCAAGTTTGATAACTCCTACTCCTTGCTCCGCAACAAGACTCTCTACTTCCATATCTACTATACTAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223140 representing NM_001033475
Red=Cloning site Green=Tags(s) MSDRQAAEGPAFWSPAARRGSAGGVGDRRGVEESQAAASEKEDLESTNVSSPLASASDPAAESSPYRPQM VSPASKDTTEDLQNVAGASEGQAPGEQAALPAGQTQVLSEMAKYQAPQRPEDTVMIQSEHTGAIDVLPAD LESADLLGDHRKVSPPLMAPPCVWTFAKVKEFKSKLGKEKNSRLVVKRGEVVTIRVPTHPEGKRVCWEFA TDDYDIGFGVYFDWTPVTSTDITVQVSDSSEDEEEEEDEEEEIEEPVPVGDVERGSRSSLRGRYGEVMPV YRRDSHRDVQAGSHDYPGEGIYLLKFDNSYSLLRNKTLYFHIYYTS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001033475 |
ORF Size | 978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033475.3, NP_001028647.1 |
RefSeq Size | 7179 bp |
RefSeq ORF | 981 bp |
Locus ID | 382620 |
UniProt ID | Q3UHI4 |
Cytogenetics | 12 D2 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214772 | Tmed8 (untagged) - Mouse transmembrane emp24 domain containing 8 (Tmed8), (10ug) |
USD 330.00 |
|
MR223140 | Tmed8 (Myc-DDK-tagged) - Mouse transmembrane emp24 domain containing 8 (Tmed8) |
USD 300.00 |
|
MR223140L3 | Lenti ORF clone of Tmed8 (Myc-DDK-tagged) - Mouse transmembrane emp24 domain containing 8 (Tmed8) |
USD 600.00 |
|
MR223140L4 | Lenti ORF clone of Tmed8 (mGFP-tagged) - Mouse transmembrane emp24 domain containing 8 (Tmed8) |
USD 600.00 |
{0} Product Review(s)
Be the first one to submit a review