Postn (NM_015784) Mouse Tagged ORF Clone

CAT#: MG223073

Postn (tGFP-tagged) - Mouse periostin osteoblast specific factor (Postn), (10ug)

Product Images

Circular map for MG223073

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Postn
• Synonyms: A630052E07Rik; AI747096; Os; OSF; OSF-2; Osf2; p; Pe; PLF; PN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223073 representing NM_015784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCCTCTCCTGCCCTTATATGCTCTGCTGCTGCTGTTCCTGTGTGATATTAACCCTGCAAATGCCA
  ACAGTTACTATGACAAGGTCCTGGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAACGTCTGTGC
  CCTCCAGCAAATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC
  TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCTGGCTATATGAGAATGGAAGGGATGAAAGGCT
  GCCCCGCAGTGATGCCTATTGACCATGTTTATGGCACGCTGGGCATTGTGGGAGCCACTACCACTCAGCA
  CTACTCCGATGTCTCGAAGCTGAGAGAAGAGATTGAAGGAAAAGGGTCATACACGTACTTCGCGCCGAGT
  AACGAGGCTTGGGAGAACCTGGATTCTGACATTCGCAGAGGACTGGAGAACAATGTCAATGTTGAGCTAC
  TGAATGCCTTACACAGCCACATGGTTAATAAGAGAATGTTAACCAAGGACCTGAAACACGGCATGGTTAT
  TCCTTCAATGTACAACAATCTGGGGCTTTTTATTAACCATTATCCCAATGGGGTTGTCACTGTGAACTGT
  GCTCGAGTCATCCATGGGAACCAGATTGCCACAAATGGTGTCGTCCATGTCATTGACCGTGTCCTGACAC
  AAATTGGTACCTCCATCCAAGACTTCCTTGAAGCAGAAGACGACCTTTCATCATTTAGAGCAGCCGCCAT
  CACCTCTGACCTCTTGGAGTCCCTTGGAAGAGATGGTCACTTCACGCTCTTTGCTCCCACCAATGAAGCT
  TTCGAGAAACTGCCACGAGGTGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA
  AGTACCACATCCTAAATACCCTCCAGTGCTCTGAGGCCATCACTGGAGGAGCCGTGTTTGAGACCATGGA
  AGGAAACACTATTGAGATAGGGTGCGAAGGGGACAGTATCTCCATTAACGGAATCAAGATGGTGAACAAG
  AAAGACATTGTGACTAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCCAAAC
  AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACCGACCTGGTAGCCCAATTAGGCTTGGCATC
  CTCTCTGAAGCCAGATGGAGAGTACACCTTATTAGCACCTGTGAACAATGCGTTCTCTGATGACACTCTG
  AGCATGGACCAACGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTTGGCCTTAGCG
  ACCTCTACAATGGACAGATACTGGAAACCATTGGAGGCAAACAACTCCGAGTCTTTGTGTATCGGACGGC
  TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGAAGGAATGGTGCCATTCACATATTC
  CGAGAAATCATCCAACCAGCAGAGAAATCCCTGCACGACAAGCTGCGGCAAGACAAGCGCTTTAGCATCT
  TCCTCAGCCTCCTTGAAGCTGCAGATTTGAAAGATCTCCTGACACAGCCCGGAGATTGGACCTTGTTTGC
  ACCAACCAATGATGCCTTCAAGGGAATGACTAGCGAAGAAAGGGAGCTTCTGATTGGGGATAAAAATGCT
  CTCCAAAACATCATTCTTTATCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTCGAACCCGGAGTCA
  CTAATATCCTGAAGACCACACAGGGAAGCAAAATCTATCTGAAAGGAGTAAACGAAACGCTTCTAGTGAA
  TGAGTTGAAGTCCAAAGAATCTGACATCATGACGACAAATGGTGTCATCCACGTCGTGGACAAACTCCTC
  TATCCAGCAGATATTCCAGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC
  AAATCAAGTTTGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATAGACCTGCAATGACGAA
  GATCCAAATTGAAGGTGATCCCGACTTCAGGCTGATTAAAGAAGGCGAAACGGTGACAGAAGTGATCCAC
  GGAGAGCCAGTCATTAAAAAGTACACCAAAATCATAGATGGAGTTCCTGTTGAAATAACTGAAAAACAGA
  CTCGGGAAGAACGAATCATTACAGGTCCTGAGATAAAATATACCAGGATTTCCACAGGAGGTGGAGAAAC
  AGGAGAGACCTTGCAGAAATTCTTGCAAAAAGAGGTCTCCAAGGTCACAAAGTTCATTGAAGGTGGCGAT
  GGTCACTTATTTGAAGATGAGGAGATTAAAAGACTGCTTCAGGGAGACACACCTGCAAAGAAGATACCAG
  CCAACAAAAGGGTTCAAGGGCCTAGAAGACGATCAAGAGAAGGCCGTTCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223073 representing NM_015784
  Red=Cloning site Green=Tags(s)
  
  MVPLLPLYALLLLFLCDINPANANSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAI
  CGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPS
  NEAWENLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNC
  ARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFLEAEDDLSSFRAAAITSDLLESLGRDGHFTLFAPTNEA
  FEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNK
  KDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTL
  SMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIF
  REIIQPAEKSLHDKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTSEERELLIGDKNA
  LQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYLKGVNETLLVNELKSKESDIMTTNGVIHVVDKLL
  YPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYRPAMTKIQIEGDPDFRLIKEGETVTEVIH
  GEPVIKKYTKIIDGVPVEITEKQTREERIITGPEIKYTRISTGGGETGETLQKFLQKEVSKVTKFIEGGD
  GHLFEDEEIKRLLQGDTPAKKIPANKRVQGPRRRSREGRSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015784
• ORF Size: 2433 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015784.3, NP_056599.1
• RefSeq Size: 3187 bp
• RefSeq ORF: 2436 bp
• Locus ID: 50706
• UniProt ID: Q62009
• Cytogenetics: 3 C
• Gene Summary: This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]