Setdb1 (NM_001163642) Mouse Tagged ORF Clone

CAT#: MG222934

Setdb1 (tGFP-tagged) - Mouse SET domain bifurcated 1 (Setdb1) transcript variant 3, (10ug)

Product Images

Circular map for MG222934

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Setdb1
• Synonyms: AU022152; ESET; KMT1E; mKIAA0067
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222934 representing NM_001163642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCCTCCCTGGGTGCATGAGTTTGGCTGCAGCGCCAGCTGCAGCTGACTCTGCAGAGATTGCTG
  AGCTGCAGCAGGCGGTGGTTGAAGAGCTGGGTATCTCTATGGAGGAACTTCGTCAGTACATTGATGAGGA
  ACTGGAAAAGATGGACTGCATACAGCAGCGCAAGAAGCAGCTCGCAGAGCTGGAGACGTGGGTACTACAG
  AAAGAGTCTGAAGTGGCTTATGTTGATCGGCTGTTTGATGATGCATCCAGGGAAGTGACTAACTGTGAGT
  CTTTGGTGAAGGATTTCTACTCTAAGCTGGGACTACAGTATCATGACAGTAGCTCTGAGGATGAAGCTTC
  CCGGCCCACAGAGATCATTGAGATTCCTGATGAAGATGATGATGTCCTCAGTATTGATTCAGGTGATGCT
  GGGAGCAGAACTCCAAAAGACCAGAAGCTTCGTGAAGCTATGGCTGCCTTAAGAAAATCAGCTCAAGATG
  TCCAGAAGTTCATGGATGCTGTCAACAAGAAAAGCAGTTCTCAAGATCTACATAAAGGAACCTTGGGTCA
  GGTGTCTGGAGAACTGAGCAAAGATGGGGACCTGATAGTCAGCATGCGGATTCTGGGCAAGAAGAGGACT
  AAGACATGGCACAAAGGCACCCTTATTGCCATCCAGACTGTTGGGCTAGGAAAAAAATACAAAGTGAAAT
  TTGACAACAAAGGAAAGAGTCTGCTATCTGGGAACCATATTGCCTATGATTACCACCCTCCCGCTGACAA
  GCTGTTTGTGGGCAGTCGAGTGGTGGCCAAGTACAAAGATGGAAATCAGGTCTGGCTTTATGCTGGCATT
  GTAGCTGAGACCCCTAACGTCAAGAACAAGCTCAGATTTTTAATTTTTTTTGATGATGGCTATGCTTCCT
  ATGTCACTCAGTCAGAGCTTTATCCCATTTGCCGACCACTAAAAAAGACTTGGGAGGACATAGAAGATAG
  CTCCTGCCGAGACTTCATAGAGGAATATATCACTGCCTATCCAAACCGCCCAATGGTACTTCTCAAGAGT
  GGGCAGCTTATCAAGACTGAGTGGGAAGGCACATGGTGGAAGTCTCGAGTTGAAGAGGTGGATGGCAGCC
  TAGTCAGGATCCTCTTTCTGGATGACAAAAGATGTGAGTGGATATATCGAGGCTCTACACGCCTGGAACC
  TATGTTTAGTATGAAGACATCCTCAGCCTCTGCAATGGAGAAGAAGCAAGGGGGGCAACTCAGAACCCGT
  CCTAATATGGGTGCTGTGAGGAGCAAAGGTCCTGTTGTTCAGTATACACAGGATCTAACTGGTACTGGAA
  TCCAGTTTAAGCCCATGGAGCCCCTACAGCCTATAGCTCCACCGGCCCCACTTCCTATACCTCCTCTTTC
  CCCCCAAGCAGCTGACACTGACTTAGAAAGCCAACTTGCACAATCACGGAAACAAGTAGCCAAGAAGAGC
  ACATCATTCCGACCAGGATCTGTGGGCTCCGGCCATTCCTCCCCTACTTCATCCACACTCAGTGAAAATG
  TGTCTGCTGGGAAACTTGGGATAAACCAGACATATCGGTCACCTTTGGCCTCAGTAACATCTACCCCAGC
  ATCTGCAGCCCCTCCAGTCCCTCCAGTCCCACCAGGGCCTCCAACCCCTCCAGGGCCTCCAGCTCCTCCA
  GGGCCTCTAGCTCCTCCAGCCTTCCATGGCATGTTAGAGCGGGCACCAGCTGAGCCCTCCTACCGAGCCC
  CCATGGAGAAGCTTTTCTATTTACCTCATGTCTGCAGTTACACTTGTTTGTCCCGGATCAGACCCATGAG
  AAACGAACAGTATCGGGGCAAGAACCCTCTATTAGTTCCACTTCTGTATGACTTCCGGAGGATGACAGCA
  CGGCGCAGAGTTAACCGCAAAATGGGCTTTCATGTAATCTATAAGACACCCTGTGGTCTCTGCCTTCGGA
  CGATGCAGGAGATAGAGCGCTACCTTTTTGAGACTGGCTGTGACTTTCTGTTCCTGGAGATGTTCTGTTT
  GGATCCATATGTTCTTGTTGACAGAAAGTTTCAACCCTTTAAGCCTTTTTACTATATTTTGGACATCACC
  TATGGCAAGGAAGATGTTCCCCTGTCCTGTGTTAATGAGATTGACACAACTCCCCCACCCCAGGTGGCCT
  ACAGCAAGGAACGCATTCCTGGCAAGGGTGTTTTCATTAACACAGGCCCTGAATTTCTGGTTGGCTGTGA
  CTGCAAGGATGGGTGTCGGGATAAATCCAAATGTGCCTGCCACCAGCTAACTATCCAGGCCACAGCCTGT
  ACCCCAGGGGGCCAAGTCAACCCTAACTCTGGCTACCAGTATAAAAGACTAGAAGAGTGTCTGCCCACAG
  GGGTTTATGAGTGTAACAAACGCTGCAATTGTGACCCAAACATGTGCACAAATCGGTTGGTGCAGCATGG
  TCTGCAGGTTCGACTACAGCTGTTTAAGACACAGAACAAGGGCTGGGGTATCCGCTGCTTGGATGATATT
  GCCAAAGGCTCTTTTGTCTGCATTTATGCAGGCAAAATCCTGACAGATGACTTTGCAGACAAAGAAGGCC
  TGGAGATGGGTGATGAGTACTTTGCAAATCTGGACCACATTGAAAGTGTGGAGAACTTCAAGGAAGGATA
  TGAGAGTGATGTCCCCACTTCCTCTGACAGCAGTGGGGTAGATATGAAGGACCAGGAAGATGGCAACAGC
  GGTTCAGAGGACCCTGAAGAATCCAATGATGACAGCTCTGATGATAACTTCTGTAAGGATGAGGACTTCA
  GCACCAGTTCAGTGTGGCGTAGCTATGCTACCCGGAGGCAGACTCGGGGTCAAAAGGAGAATGAATTGTC
  TGAGATGACTTCCAAGGACTCCCGCCCCCCAGACCTCGGGCCTCCACATGTTCCTATCCCTTCCTCAGTA
  TCTGTAGGGGGCTGCAATCCACCTTCCTCTGAAGAGACACCCAAGAACAAGGTGGCCTCGTGGTTGAGTT
  GCAATAGTGTCAGTGAAGGTGGATTTGCTGACTCTGACAGCCGTTCTTCCTTCAAGACTAGTGAAGGTGG
  AGATGGCCGTGCTGGGGGAGGCCGGGGAGAGGCTGAAAGGGCCTCTACCTCAGGATTGAGCTTCAAGGAT
  GAAGGAGACAATAAGCAGCCTAAAAAAGAGGACCCTGAGAACCGAAACAAGATGCCAGTAGTTACTGAAG
  GCTCTCAGAATCATGGACATAATCCTCCCATGAAGTCTGAAGGGCTTCGCCGATCAGCTAGTAAAATGTC
  TGTGCTCCAGAGCCAGCGAGTTGTGACTTCTACTCAGTCAAACCCTGATGACATCCTGACACTGTCCAGC
  AGCACAGAGAGTGAGGGGGAAAGTGGAACCAGCCGAAAGCCCACTGCTGGTCACACTTCAGCCACAGCTG
  TTGATAGTGATGACATCCAGACCATCTCTTCTGGCTCTGACGGTGATGACTTTGAGGACAAGAAGAACTT
  GTCAGGACCAACAAAGCGCCAGGTGGCAGTAAAATCAACCCGAGGCTTTGCTCTTAAATCAACCCATGGT
  ATTGCCATTAAATCAACCAACATGGCTTCCGTGGACAAGGGGGAGAGTGCACCAGTTCGTAAGAACACAC
  GCCAGTTCTATGATGGTGAAGAGTCTTGCTACATCATTGATGCCAAACTTGAAGGCAACCTAGGCCGCTA
  CCTCAATCACAGTTGCAGCCCCAACCTGTTTGTCCAGAATGTGTTTGTGGATACCCATGATCTTCGCTTC
  CCTTGGGTGGCCTTCTTTGCCAGCAAGAGAATCCGGGCTGGAACAGAACTCACTTGGGACTACAACTACG
  AAGTGGGCAGTGTGGAAGGCAAGGAGCTGCTGTGCTGCTGTGGGGCCATTGAATGCAGAGGGAGACTTCT
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222934 representing NM_001163642
  Red=Cloning site Green=Tags(s)
  
  MSSLPGCMSLAAAPAAADSAEIAELQQAVVEELGISMEELRQYIDEELEKMDCIQQRKKQLAELETWVLQ
  KESEVAYVDRLFDDASREVTNCESLVKDFYSKLGLQYHDSSSEDEASRPTEIIEIPDEDDDVLSIDSGDA
  GSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLGQVSGELSKDGDLIVSMRILGKKRT
  KTWHKGTLIAIQTVGLGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLFVGSRVVAKYKDGNQVWLYAGI
  VAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDSSCRDFIEEYITAYPNRPMVLLKS
  GQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASAMEKKQGGQLRTR
  PNMGAVRSKGPVVQYTQDLTGTGIQFKPMEPLQPIAPPAPLPIPPLSPQAADTDLESQLAQSRKQVAKKS
  TSFRPGSVGSGHSSPTSSTLSENVSAGKLGINQTYRSPLASVTSTPASAAPPVPPVPPGPPTPPGPPAPP
  GPLAPPAFHGMLERAPAEPSYRAPMEKLFYLPHVCSYTCLSRIRPMRNEQYRGKNPLLVPLLYDFRRMTA
  RRRVNRKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLFLEMFCLDPYVLVDRKFQPFKPFYYILDIT
  YGKEDVPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGCDCKDGCRDKSKCACHQLTIQATAC
  TPGGQVNPNSGYQYKRLEECLPTGVYECNKRCNCDPNMCTNRLVQHGLQVRLQLFKTQNKGWGIRCLDDI
  AKGSFVCIYAGKILTDDFADKEGLEMGDEYFANLDHIESVENFKEGYESDVPTSSDSSGVDMKDQEDGNS
  GSEDPEESNDDSSDDNFCKDEDFSTSSVWRSYATRRQTRGQKENELSEMTSKDSRPPDLGPPHVPIPSSV
  SVGGCNPPSSEETPKNKVASWLSCNSVSEGGFADSDSRSSFKTSEGGDGRAGGGRGEAERASTSGLSFKD
  EGDNKQPKKEDPENRNKMPVVTEGSQNHGHNPPMKSEGLRRSASKMSVLQSQRVVTSTQSNPDDILTLSS
  STESEGESGTSRKPTAGHTSATAVDSDDIQTISSGSDGDDFEDKKNLSGPTKRQVAVKSTRGFALKSTHG
  IAIKSTNMASVDKGESAPVRKNTRQFYDGEESCYIIDAKLEGNLGRYLNHSCSPNLFVQNVFVDTHDLRF
  PWVAFFASKRIRAGTELTWDYNYEVGSVEGKELLCCCGAIECRGRLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163642
• ORF Size: 3921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163642.1, NP_001157114.1
• RefSeq Size: 4641 bp
• RefSeq ORF: 3924 bp
• Locus ID: 84505
• Cytogenetics: 3 F2.1