Cacna2d4 (NM_001033382) Mouse Tagged ORF Clone

CAT#: MG222860

Cacna2d4 (tGFP-tagged) - Mouse calcium channel voltage-dependent alpha 2/delta subunit 4 (Cacna2d4), (10ug)

Product Images

Circular map for MG222860

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cacna2d4
• Synonyms: 5730412N02Rik; BE686333
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222860 representing NM_001033382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGAGTTCCTGTGGCAGCAAGTTATCTCCCAGCAGGGGCTCTGTCCTCCTCCCCTGCCCCTCCC
  CCAGTGGGAACACCATGGCAAGATGCCCCATGCTGTCCTCTAGCCACAACCAAGACCACAGTGGACGGTG
  GACAGCAGTGTGGACCTCCCTGTGGAAGACGCCAATCATCCTGTGGCTGCTGCTCTCGGACACCTCCCTG
  CCTACCGTGAGGGGCCAGACCACAGTTCCTCTGGAAACAGTGAAGCTGTGGGCCGATACCTTCGGCAGGA
  ATCTGTACAACACGGTGACCAGATATTCAGGCTCCCTCCTGCTGCAGAAGAAGTACAAGGATGCAGAACC
  CAGTCTGAAGATCAAAGAGGTGGATGGCTTGGAGCTGGTGAAGAAGTTCTCGGAGGACATGGAGACCATG
  CTTCGGAGGAAAGTCGAGGCTGTTGAGAGCCTGGTGGAGGCCGCTGAGGAGGCTGACCTGAATCATGAGT
  TCAATGCATCTCTGGTGTTTAACTACTACAACTCGGTACTAATCAATGAGAAGGATGACAAGGGCAACTA
  CGTGGAGCTGGGAGCTGAGTTTCTTCTCGAGTCCGACGCGCACTTCAGCAACCTGAGGGTGAATGTCTCC
  ATGAGCAGCGTTCAACTGCCCACCAACGTGTACAACAAAGACCCAGACATTTTAAATGGCGTGTATATGT
  CCGAAGCCCTGAACCCCGTGTTTGTGGAGAACTTTCAGAGAGACCCTACATTGACATGGCAGTATTTCGG
  CAGCTCAACTGGGTTCTTCAGGATCTATCCAGGAATAAAGTGGATGCCTGATGAAAACGGAGTCATCGCC
  TTCGACTGCAGAAACCGTGGCTGGTACATACAAGCTGCCACATCTCCCAAAGACATCGTAATTCTGGTGG
  ACATTAGCGGCAGCATGAAGGGGCTGAGGATGGCTATCGCCAAGCACACCATTACCACCATATTGGACAC
  CCTGGGAGAGAACGACTTCGTGAACATCATCGCGTACAATGACTACGTCCACTACATTGAGCCCTGCTTC
  AAAGGCATCCTTGTCCAAGCAGATCGAGACAACCGAGAGCATTTCAAGCAGTTGGTGGATGAGCTGATGG
  TCAAAGGTGTGGGTGTCGTGAGTCAAGCTCTAATTGAAGCTTTCGAGATCCTGAAACAGTTCCAAGAGTC
  CAAACAAGGAAGTCTCTGCAACCAGGCCATCATGCTTATCACCGATGGGGCTGTGGAAGACTATGAGCCC
  GTGTTTGAGACCTATAACTGGCCAGACCGAAAGGTCCGAGTTTTCACTTACCTTATTGGAAGAGAAGTGA
  CTTTCGCTGACCGCATGAAGTGGATCGCCTGCAACAATAAAGGCTACTACACACAGATCTCCACACTGGC
  GGATGCGCAGGAGAGCGTGATGGAGTACCTGCATGTGCTTAGCCGCCCCATGGTCATCAACCATGACCAC
  GACATCATCTGGACAGAGGCTTACATGGACAGCCGGCTCTTCACATCAGAGGCACAGAGCCTGATGCTCC
  TCACCACAGTGGCCATGCCTGTCTTCAGCAAAAAGAATGAAACAAGATCCCATGGCATTCTCCTGGGTGT
  AGTGGGCTCTGACGTGACCCTAAGAGAGCTCATGAAGCTGGCACCCCGATATAAGCTTGGGGTGCATGGC
  TATGCCTTCTTGAACACTAACAATGGCTACATCCTCTCTCATCCTGACCTCCGACCTTTGTACAGAGAAG
  GCAAGAAGCTGAGACCCAAACCCAACTACAACAGTGTGGACCTCTCAGAAGTGGAGTGGGAGGACCAAGC
  TGAAATTCTGAGGACCGCCATGATCAATGGGGAAACCGGGTCTCACTCCATGGACGTGAAGGTGCCACTG
  GACAAAGGGAAGCGAGTTCTATTCCTGACCAATGACTACTTCTTCACAGACATCAGTGACACACCTTTCA
  GCCTGGGAGTGGTGCTCACCAGAGGTCATGGAGAATACATCCTCCTGGGGAACACATCTGTGGAAGAAGG
  CCTGCACGACTTGCTTCATCCGGATCTGACCCTGGCCAGTGACTGGATCTACTGTATCACAGATATCGAC
  CCGGACCACCGGAAACTCAGCCAACTGGAAGCTGTGGTTCGTTTCCTGACAGGGGTGGATCCAGACCTGG
  AGTGTGACGAGGAACTGGTGCGGGAGGTGCTGTTTGATGCGGTGGTAACCGCCCCCATGGAAGCCTATTG
  GACAGCACTGGCACTCAACATCTCTGAAGAGTCAGAGCCTGGCGTGGATGTGGCCTTCCTGGGAACCCGG
  GCTGGCCTTCTAAGAAGAAGCTTGTTCGTAGGCTCCGAGAAAGTCTCTGACAGGAAGTTCCTGACCCCTG
  AAGATGAAGCCAGTATTTTTACCATGGACCACTTCCCGCTGTGGTATCGCCAGGCCTCTGAGCAGCCCCC
  CGGCAGCTTTGTCTTCAATCTCCGCTGGGCAGAGGGACCAGATAGCCCAGGCAAGCCAGTGGCGGTAAGG
  GCCAGCACAGCAGTAACTGTGACCGTGGATGGGAAGACGGCCATTGCAGCAGCTGTGGGCATCCAGATGC
  AAGCAGACTACCTCCAGCGCCAGTTCTGGGCAGCCATGCAGCAGTGCAACGCTGTAGAGGGGCCGTGCCT
  GAAGAGCTGCGAGGACACTGATCTCGACTGCTTCGTCATAGACAACAATGGCTTCGTCCTGATCTCAGAG
  AGACCCCAAGAGATGGGAAGACTTCTGGGGGAAGCGGATGGTGCTCTCATGAAGCAACTTCTCAGCATGG
  GGGTGTTCAGCCGTGTGACCATGTATGACTACCAGGCCATGTGCAAGCCCCCAGATCATCACCACAGTGC
  GGCCGAATCCCTGTTCAGTCCTCTCTCTGCCTTCTTGATGGTGGCCAGGTGGCTACTGCATGAATGTCTC
  CTATTCCTGTTAGAGTGGAGTGCCTGGGGATCTTGGCAGGACAAAGGGTCAGAGGCCAAATCTGTCTTCC
  ATCACTCCCACAAGCACAAGAAGCAGGACCTGCTGCACCCCTGTGACACAGAGTACCCAGTGTTCGTGCA
  TCAGACGGCCATCCAGGAAGCCAATGGCATCATCGAGTGTGGGGGCTGCCAGAAGACATTCGTGATGCAA
  CAGATTCCCCGAAGCAACCTGCTCCTGCTCGTGACAGACCGCACCTGTGACTGCAGCGCCCACTCTCCCA
  TCCTCCAGGAGGCCACAGAAGTCAAATATAATGCCTCTGTCAAGTGTGACAGGATGCGCTCCCAGAAGCC
  CCGGAGACGACCAGGCTCCTGCCATGCCTTCCATCCAGAGGAGAATGCCCAGGACTGTGGCGGTGCTTCA
  GACACTCTGCCTTCATCCCCTCTGCTCCTGCTATCACTGGGGGCCTGGCTGCTTCCACCCCAGCTTCTGT
  GG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222860 representing NM_001033382
  Red=Cloning site Green=Tags(s)
  
  MPRSSCGSKLSPSRGSVLLPCPSPSGNTMARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSL
  PTVRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELVKKFSEDMETM
  LRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYVELGAEFLLESDAHFSNLRVNVS
  MSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQRDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIA
  FDCRNRGWYIQAATSPKDIVILVDISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCF
  KGILVQADRDNREHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEP
  VFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLHVLSRPMVINHDH
  DIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLGVVGSDVTLRELMKLAPRYKLGVHG
  YAFLNTNNGYILSHPDLRPLYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGETGSHSMDVKVPL
  DKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEGLHDLLHPDLTLASDWIYCITDID
  PDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLFDAVVTAPMEAYWTALALNISEESEPGVDVAFLGTR
  AGLLRRSLFVGSEKVSDRKFLTPEDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVR
  ASTAVTVTVDGKTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISE
  RPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECL
  LFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQ
  QIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGAS
  DTLPSSPLLLLSLGAWLLPPQLLW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033382
• ORF Size: 3432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033382.2, NP_001028554.2
• RefSeq Size: 5803 bp
• RefSeq ORF: 3435 bp
• Locus ID: 319734
• UniProt ID: Q5RJF7
• Cytogenetics: 6 F1
• Gene Summary: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel.[UniProtKB/Swiss-Prot Function]