Hcrt (NM_010410) Mouse Tagged ORF Clone
CAT#: MG222595
- TrueORF®
Hcrt (tGFP-tagged) - Mouse hypocretin (Hcrt), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010410" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Hcrt |
Synonyms | PPOX |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222595 representing NM_010410
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTCCTTCTACAAAGGTTCCCTGGGCCGCCGTGACGCTGCTGCTGCTGCTACTGCTGCCGCCGG CGCTGCTGTCGCTTGGGGTGGACGCACAGCCTCTGCCCGACTGCTGTCGCCAGAAGACGTGTTCCTGCCG TCTCTACGAACTGTTGCACGGAGCTGGCAACCACGCTGCGGGTATCCTGACTCTGGGAAAGCGGCGGCCT GGACCTCCAGGCCTCCAGGGACGGCTGCAGCGCCTCCTTCAGGCCAACGGTAACCACGCAGCTGGCATCC TGACCATGGGCCGCCGCGCAGGCGCAGAGCTAGAGCCACATCCCTGCTCTGGTCGCGGCTGTCCGACCGT AACTACCACCGCTTTAGCACCCCGGGGAGGGTCCGGAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222595 representing NM_010410
Red=Cloning site Green=Tags(s) MNFPSTKVPWAAVTLLLLLLLPPALLSLGVDAQPLPDCCRQKTCSCRLYELLHGAGNHAAGILTLGKRRP GPPGLQGRLQRLLQANGNHAAGILTMGRRAGAELEPHPCSGRGCPTVTTTALAPRGGSGV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010410 |
ORF Size | 390 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010410.2, NP_034540.1 |
RefSeq Size | 584 bp |
RefSeq ORF | 393 bp |
Locus ID | 15171 |
UniProt ID | O55241 |
Cytogenetics | 11 63.6 cM |
Gene Summary | This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors Hcrtr1 and Hcrtr2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208652 | Hcrt (untagged) - Mouse hypocretin (Hcrt), (10ug) |
USD 165.00 |
|
MR222595 | Hcrt (Myc-DDK-tagged) - Mouse hypocretin (Hcrt) |
USD 150.00 |
|
MR222595L3 | Lenti ORF clone of Hcrt (Myc-DDK-tagged) - Mouse hypocretin (Hcrt) |
USD 450.00 |
|
MR222595L4 | Lenti ORF clone of Hcrt (mGFP-tagged) - Mouse hypocretin (Hcrt) |
USD 450.00 |
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