Mecom (NM_007963) Mouse Tagged ORF Clone
CAT#: MG222560
- TrueORF®
Mecom (tGFP-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_007963" in other vectors (6)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Mecom |
Synonyms | D630039M04Rik; Evi-1; Evi1; Jbo; Mds; Mds1; Mds1-Evi1; Prdm3; Znfpr1b1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222560 representing NM_007963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGTGAAGAGGACCCGCACGAACCCATGGCGCCTGACATCCACGAAGAACGGCAGCACCGCTGTG AGGACTGTGACCAGCTCTTTGAATCCAAGGCAGAGCTAGCCGATCACCAGAAGTTCCCATGCAGCACACC TCACTCGGCCTTCTCCATGGTGGAGGAGGACTTGCAACAAAACCTGGAGAGTGAGAGCGATCTCCGAGAG ATCCATGGCAACCAGGACTGTAAGGAATGTGACCGAGTTTTCCCCGATCTGCAAAGCTTGGAGAAGCACA TGCTGTCACATACTGAGGAGAGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAGTCCAA TTTAATTCGCCACCAGATGTCACATGACAGTGGAAAGCACTATGAGTGTGAAAACTGTGCCAAGGTTTTC ACGGACCCTAGCAACCTTCAGCGACACATTCGATCTCAGCATGTTGGTGCCCGGGCTCATGCTTGCCCCG AGTGTGGTAAAACATTTGCCACTTCGTCAGGCCTCAAACAGCACAAGCACATCCACAGCAGTGTGAAGCC CTTTATCTCATTCTCTCAATCAATGTACCCATTTCCTGATAGAGACTTGAGGTCGTTACCTTTGAAAATG GAGCCCCAATCACCAAGTGAAGTTAAGAAACTGCAGAAGGGAAGCTCTGAGTCCCCTTTTGACCTCACCA CTAAGAGAAAGGATGAGAAGCCCTTGACTTCAGGCCCCTCGAAGCCTTCAGGAACACCAGCCACAAGCCA AGACCAGCCCCTGGATCTAAGTATGGGCAGTAGGGGTAGAGCCAGTGGGACAAAGTTGACTGAGCCTCGA AAAAACCATGTGTTTGGGGAAAAGAAAGGAAGCAACATGGATACTAGGCCATCTTCAGATGGCTCCTTGC AGCATGCCAGACCCACTCCCTTCTTCATGGACCCCATTTATAGAGTAGAGAAAAGAAAGTTAACTGACCC GCTTGAAGCTTTGAAAGAAAAATACTTGAGACCTTCTCCAGGATTCTTGTTTCACCCGCAAATGTCAGCA ATTGAGAACATGGCAGAAAAGCTGGAAAGCTTCAGCGCCCTCAAACCTGAGGCCAGCGAGCTCCTGCAGT CCGTGCCCTCCATGTTCAGCTTCCGAGCTCCTCCCAACACCCTGCCAGAGAACCTGCTGCGGAAGGGGAA AGAGCGCTACACCTGCAGGTACTGTGGCAAGATATTTCCAAGGTCTGCGAACCTAACACGGCACTTGAGA ACCCACACAGGAGAGCAACCTTACAGATGCAAATACTGTGATAGATCATTCAGCATTTCTTCCAACCTGC AGCGACATGTGCGCAACATCCACAACAAGGAGAAGCCATTTAAGTGTCATTTATGTGACAGATGTTTTGG TCAACAAACCAATCTTGACAGACACCTGAAGAAACATGAGAACGGCAACATGTCTGGGACGGCAACGTCC TCGCCTCACTCAGAGCTAGAAAGCGCAGGCGCAATCCTGGATGACAAAGAAGATGCGTACTTTACAGAGA TCCGCAATTTCATCGGGAACAGCAACCATGGTAGCCAGTCTCCTCGGAACATGGAAGAGAGGATGAATGG CAGTCACTTCAAGGATAAAAAGGCTTTGGCAACCAGCCAAAATTCAGATTTATTGGATGATGAAGAAGTA GAAGATGAGGTGTTGTTGGATGAGGAGGATGAAGACAATGATATTCCTGGAAAGCCCAGAAAGGAGCTAG GGGTGACTCGTTTAGACGAGGAGATCCCGGAGGATGACTACGAAGAAGCTGGTGCCCTGGAGATGAGCTG TAAGGCGTCCCCGGTGAGGTATAAAGAGGAAGACTATAAATCTGGCCTTTCTGCTCTAGATCACATAAGG CACTTCACAGATAGCCTCAAAATGAGGGAAATGGAAGAGAATCAATACACTGACGCTGAGCTGTCCTCCA TTAGTTCTTCTCATGTGCCAGAGGAGCTTAAACAGACGTTACACAGAAAGTCCAAATCACAGGCATATGC TATGATGTTGTCACTGTCTGACAAGGATTCCCTCCATCCCACCTCCCACAGTTCCTCCAACGTGTGGCAC AGCATGGCAAGGGCTGCAGCAGAATCCAGTGCCATCCAGTCCATAAGCCATGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222560 representing NM_007963
Red=Cloning site Green=Tags(s) MKSEEDPHEPMAPDIHEERQHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLRE IHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVF TDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFISFSQSMYPFPDRDLRSLPLKM EPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTSGPSKPSGTPATSQDQPLDLSMGSRGRASGTKLTEPR KNHVFGEKKGSNMDTRPSSDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSA IENMAEKLESFSALKPEASELLQSVPSMFSFRAPPNTLPENLLRKGKERYTCRYCGKIFPRSANLTRHLR THTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATS SPHSELESAGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNMEERMNGSHFKDKKALATSQNSDLLDDEEV EDEVLLDEEDEDNDIPGKPRKELGVTRLDEEIPEDDYEEAGALEMSCKASPVRYKEEDYKSGLSALDHIR HFTDSLKMREMEENQYTDAELSSISSSHVPEELKQTLHRKSKSQAYAMMLSLSDKDSLHPTSHSSSNVWH SMARAAAESSAIQSISHV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007963 |
ORF Size | 2154 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007963.2, NP_031989.2 |
RefSeq Size | 4429 bp |
RefSeq ORF | 2157 bp |
Locus ID | 14013 |
Cytogenetics | 3 12.66 cM |
Gene Summary | Isoform 1: Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220987 | Mecom (untagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1, (10ug) |
USD 658.00 |
|
MR222560 | Mecom (Myc-DDK-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1 |
USD 723.00 |
|
MR222560L1 | Lenti ORF clone of Mecom (Myc-DDK-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1 |
USD 1,023.00 |
|
MR222560L2 | Lenti ORF clone of Mecom (mGFP-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1 |
USD 1,023.00 |
|
MR222560L3 | Lenti ORF clone of Mecom (Myc-DDK-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1 |
USD 1,023.00 |
|
MR222560L4 | Lenti ORF clone of Mecom (mGFP-tagged) - Mouse MDS1 and EVI1 complex locus (Mecom), transcript variant 1 |
USD 1,023.00 |
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