Ablim1 (NM_001103177) Mouse Tagged ORF Clone
CAT#: MG222516
- TrueORF®
Ablim1 (tGFP-tagged) - Mouse actin-binding LIM protein 1 (Ablim1) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001103177" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ablim1 |
Synonyms | 2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222516 representing NM_001103177
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAAGAAAAAGTGGCCCATCCTCAGGACCCTCACCACTCGTCGGAGAAACCTGTCATTCACTGCC ATAAATGCGGGGAGCCATGCAAGGGCGAAGTGCTACGGGTGCAGACCAAGCATTTCCACATCAAATGCTT CACCTGCAAAGTGTGCGGCTGCGACCTGGCCCAGGGGGGCTTCTTCATAAAGAACGGGGACTATCTGTGT ACCCTGGACTACCAGAGGATGTATGGGACACGGTGCCACGGCTGTGGGGAGTTCGTGGAGGGCGAGGTGG TAACTGCCCTGGGCAAGACCTACCACCCCAACTGCTTCGCCTGTACAATCTGCAAGCGCCCATTTCCACC CGGAGACCGAGTCACATTCAACGGGAGAGACTGTCTTTGTCAGCTCTGTGCACAGCCGATGTCTTCCAGC CCTAAAGAAGCCTCCTGCTCCAGCAACTGCGCCGGCTGTGGAAGGGACATTAAGAACGGACAGGCGCTGC TAGCTCTGGACAAGCAGTGGCATTTGGGCTGCTTCAAGTGCAAGTCCTGTGGGAAGGTCCTCACCGGCGA GTACATCAGCAAGGATGGCTCCCCATACTGTGAGAAAGACTACCAGGGGCTCTTTGGGGTGAAGTGTGAA GCCTGTCACCAGTTTATCACAGGAAAAGTCCTGGAGGCAGGCGACAAGCATTATCATCCCAGCTGCGCAC GATGCAGCAGATGCAATCAGATGTTCACAGAAGGGGAAGAAATGTATCTGCAAGGTTCTACCGTGTGGCA TCCCGACTGTAAGCAGTCCACTAAGACAGAGGAGAAGCTGCGGCCCACCAGGACCTCCTCTGAAAGTATC TATTCTAGACCAGGCTCCAGCATCCCTGGTTCACCAGGCCATACTATCTATGCAAAAGTAGACAATGAAA TCCTGGATTACAAGGATTTAGCAGCCATCCCCAAGGTCAAGGCGATCTATGACATCGAGCGTCCAGATCT CATTACCTATGAGCCTTTCTACACATCAGGCTATGAGGACAAGCAGGAGAGACAGAGCCTTGGAGAGTCT CCAAGGACTTTGTCTCCAACTCCATCTGCAGAAGGTTATCAAGATGTCCGGGATCGGATGATCCACAGGT CCACCAGCCAGGGCTCCATCAACTCCCCTGTGTATAGCCGGCACAGTTACACTCCAACTACGTCTCGCTC GCCCCAGCACTTCCACCGACCTGGCAATGAGCCGTCCAGCGGCCGGAACTCCCCTCTCCCCTACCGGCCC GACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTCCATGTTCCAGATCAAGGGATCA ACATTTACCGAAAACCACCCATCTACAAACAGCATGCTGCCTTGGCAGCCCAGAGCAAGGCTTCAGAAGA TATCATCAAGTTTTCCAAGTTCCCAGCAGCCCAGGCGCCAGATCCCAACGAGATACCAAAGATTGAGACG GACCACTGGCCGGGTCCCCCCTCACTTGCCGCCGTAGGAACTGACCCGAGGCGCAGATCAAGTGGCAGAG AGGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAACAGTTGATGAAGCTCAACTCAGG CCTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGAAAGAGCATCGCTGGCCAGTCGC TATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACTTCATCTCTCCCTGGTTATGGAA AGAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACAGCTACGGAGACATCAGTGGCGG AGTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAGAATGGACAGAGGAGTATCCATG CCCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTGACCAACAGAGGGCGCAACAAAA TCCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAGAAGTATTTTGGGAGATCTTTGG GATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGACATGAAGAAAAAAGCTAAACTC TTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222516 representing NM_001103177
Red=Cloning site Green=Tags(s) MVKEKVAHPQDPHHSSEKPVIHCHKCGEPCKGEVLRVQTKHFHIKCFTCKVCGCDLAQGGFFIKNGDYLC TLDYQRMYGTRCHGCGEFVEGEVVTALGKTYHPNCFACTICKRPFPPGDRVTFNGRDCLCQLCAQPMSSS PKEASCSSNCAGCGRDIKNGQALLALDKQWHLGCFKCKSCGKVLTGEYISKDGSPYCEKDYQGLFGVKCE ACHQFITGKVLEAGDKHYHPSCARCSRCNQMFTEGEEMYLQGSTVWHPDCKQSTKTEEKLRPTRTSSESI YSRPGSSIPGSPGHTIYAKVDNEILDYKDLAAIPKVKAIYDIERPDLITYEPFYTSGYEDKQERQSLGES PRTLSPTPSAEGYQDVRDRMIHRSTSQGSINSPVYSRHSYTPTTSRSPQHFHRPGNEPSSGRNSPLPYRP DSRPLTPTYAQAPKHFHVPDQGINIYRKPPIYKQHAALAAQSKASEDIIKFSKFPAAQAPDPNEIPKIET DHWPGPPSLAAVGTDPRRRSSGREEDEEELLRRRQLQEEQLMKLNSGLGQLILKEEMEKESRERASLASR YDSPLHSASHAPSSKTSSLPGYGKNGLHRPVSTDFAQYNSYGDISGGVRDYQTLPDGHMPAVRMDRGVSM PNMLEPKIFPYEMLMVTNRGRNKILRDVDRTRLERHLAPEVFWEIFGMSIQEFDKLPLWRRNDMKKKAKL F TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001103177 |
ORF Size | 2103 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001103177.3 |
RefSeq Size | 6261 bp |
RefSeq ORF | 2106 bp |
Locus ID | 226251 |
UniProt ID | Q8K4G5 |
Cytogenetics | 19 52.09 cM |
Gene Summary | May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220621 | Ablim1 (untagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 2, (10ug) |
USD 707.00 |
|
MR222516 | Ablim1 (Myc-DDK-tagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 2 |
USD 706.00 |
|
MR222516L3 | Lenti ORF clone of Ablim1 (Myc-DDK-tagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 2 |
USD 1,006.00 |
|
MR222516L4 | Lenti ORF clone of Ablim1 (mGFP-tagged) - Mouse actin-binding LIM protein 1 (Ablim1), transcript variant 2 |
USD 1,006.00 |
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