Arhgap12 (NM_029277) Mouse Tagged ORF Clone

CAT#: MG221955

Arhgap12 (tGFP-tagged) - Mouse Rho GTPase activating protein 12 (Arhgap12) transcript variant 2, (10ug)

Product Images

Circular map for MG221955

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgap12
• Synonyms: 2810011M08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221955 representing NM_029277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAGAAGTGGAAAGATCACTGCAGGACAAGCGTATATTGAGGTGGAGTATGATTATGAGTATG
  ACGCAAAGGACAGAAAGATTGTGATCAGACAAGGGGAGCGCTACCTCCTGGTGAAGAAGACCAATGATGA
  CTGGTGGCAAGTCAGACCTGATGAGAACTCAAAAGCATTTTATGTGCCAGCACAGTATGTCAAGGAGGTC
  ACTCGCAAAGCTCTGATGCCACCTGTGAAGCAGGCAACAGGACTGCCAAATAACTCTATGAAGACAATAC
  AGAGTATGCATCTCCAGAGATCGACAGAAAATGTGAACAAAATGCCTGAGCTTTCAAGTTTTGGAAAGCC
  ATCGTCATCTGTTCAAGGAACAGGTCTTATTCGTGATGCCAATCAGAATTTCGGATCCAATTATAATTCA
  GGCCAGACTCTCAACCTAAGCCTGGACCTGACCCATAATAATGGAAAGTTTAACAGTGACTCACATTCTC
  CTAAAGTTTCCAGCCAGAATAGGACACGCTTATTTGGTCATTTTCCCGGGCCAGAGTTCTTGGACATAGA
  GAAAACTAGCTTCTCTCAGGAACAGTCATGTGACTCAGCTGGAGAAGGCTCCGAAAGAATACAGCAGGAT
  TCCGAGTCTGGAGATGAACTTAGCAGCAGCTCCACTGAGCAGATGAGGGCAACCACACCCCCTAATCAAG
  GAAGGCCAGATTCTCCAGTCTATGCTAACCTACAGGAACTGAAAATATCACAGTCTGCTCTTCCTCCTCT
  TCCTGGGAGCCCAGCAATTCAAGTTAATGGGGAATGGGAGACTCACAAAGACAGTTCAGGCCGTTGTTAC
  TACTATAACCGCACAACCCAGGAGCGAACCTGGAAACCACCTCGATGGGCTCGAGATGTGAGCACAAGCC
  GAGATTTTCAGAGCCCAGGAGAGCAAGAGCCTCTTTCATCAGAAGAAAACTACCACAGCAGTTGTTTCAG
  CCAGTCAGATAGTCAGTGTGGCTCTCCTCCCAGGGGTTGGTCAGAAGAGCTGGATGAACGTGGGCATACC
  TTGTATACCAGTGACTATACTAAGGAAAAGTGGCTCAAGCATGTTGATGATCAAGGTAGACAGTATTACT
  ACAGCGCAGATGGATCTCGGTCAGAGTGGGAATTGCCAAAGTATAATGCTTCATCTCAGCAGCAAAGAGA
  AATAATTAAAAGTAGGAGCTTGGACAGGCGGCTGCAAGAACCAATAGTGTTAACTAAATGGAGACATAGC
  ACCATTGTGTTGGACTCCAACGACAAGGGTCAGGAGAAATATGGACTGTTAAATGTAACAAAAATTACTG
  AAAATGGGAAAAAAGTTCGAAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTT
  TACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTAATCAGTCCAAACCAGAATTCACAGTGGAC
  CTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAGCTGAAAA
  CTCGACAAGGAACAGAACTGCTAATTCAGTCTGATAATGATGCTGTTATTAATGATTGGTTTAAAGTTCT
  TAGTAGTACTATCAATAACCAGGTAGCAGAGGCCGATGAAGCAGCTGAAGAGGAAACACCAGATTCACCA
  GGGGTAGAAAAGCATGATAAAGAAAAGGACCAGAAGGAGCTTAAAAAACTTCGCTCAATGAAAGGATCCA
  GCATGGATTCTTCAGAGCAGAAGAAAACTAAGAAAAACTTAAAGAAGTTTCTTACCCGGCGCCCCACTTT
  GCAAGCTGTTCGTGAGAAAGGTTATATCAAAGATCAAGTATTTGGATCTAATCTTGCTAATCTGTGTCAG
  AGGGAGAATGGGACAGTGCCAAAGTTTGTGAAGTTATGCATTGAGCATGTCGAAGAGCATGGTTTAGATG
  TTGATGGTATTTACAGAGTGAGTGGCAACCTTGCCGTGATCCAGAAGCTGAGGTTCGCAGTCAATCATGA
  TGAGAAGTTGGACTTGAATGACAGTAAATGGGAAGACATTCATGTCATCACGGGAGCACTCAAAATGTTT
  TTTCGAGAACTACCAGAACCTCTTTTTACGTTTAATCATTTCAATGATTTTGTTAATGCAATTAAACAAG
  AACCGAGACAGCGTGTCACTGCAGTTAAGGACCTAATCAGACAGTTGCCAAAGCCAAATCAAGATACAAT
  GCAGATTCTGTTTAGACATCTCAAAAGGGTTATAGAAAATGGAGAAAAAAACCGAATGACCTATCAGAGC
  ATAGCGATTGTTTTTGGCCCCACCCTTCTAAAGCCAGAAAGAGAGACTGGTAATATAGCAGTTCATACTG
  TGTACCAGAACCAGATTGTAGAGCTGATTCTCCTGGAATTAAGCACTGTCTTTGGACGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221955 representing NM_029277
  Red=Cloning site Green=Tags(s)
  
  MAERSGKITAGQAYIEVEYDYEYDAKDRKIVIRQGERYLLVKKTNDDWWQVRPDENSKAFYVPAQYVKEV
  TRKALMPPVKQATGLPNNSMKTIQSMHLQRSTENVNKMPELSSFGKPSSSVQGTGLIRDANQNFGSNYNS
  GQTLNLSLDLTHNNGKFNSDSHSPKVSSQNRTRLFGHFPGPEFLDIEKTSFSQEQSCDSAGEGSERIQQD
  SESGDELSSSSTEQMRATTPPNQGRPDSPVYANLQELKISQSALPPLPGSPAIQVNGEWETHKDSSGRCY
  YYNRTTQERTWKPPRWARDVSTSRDFQSPGEQEPLSSEENYHSSCFSQSDSQCGSPPRGWSEELDERGHT
  LYTSDYTKEKWLKHVDDQGRQYYYSADGSRSEWELPKYNASSQQQREIIKSRSLDRRLQEPIVLTKWRHS
  TIVLDSNDKGQEKYGLLNVTKITENGKKVRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVD
  LKGAVIEMASKDKSSKKNVFELKTRQGTELLIQSDNDAVINDWFKVLSSTINNQVAEADEAAEEETPDSP
  GVEKHDKEKDQKELKKLRSMKGSSMDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQ
  RENGTVPKFVKLCIEHVEEHGLDVDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMF
  FRELPEPLFTFNHFNDFVNAIKQEPRQRVTAVKDLIRQLPKPNQDTMQILFRHLKRVIENGEKNRMTYQS
  IAIVFGPTLLKPERETGNIAVHTVYQNQIVELILLELSTVFGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029277
• ORF Size: 2439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029277.2, NP_083553.2
• RefSeq Size: 4959 bp
• RefSeq ORF: 2442 bp
• Locus ID: 75415
• UniProt ID: Q8C0D4
• Cytogenetics: 18 A1
• Gene Summary: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.[UniProtKB/Swiss-Prot Function]