Slc16a3 (NM_001038653) Mouse Tagged ORF Clone

CAT#: MG221679

Slc16a3 (tGFP-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters) member 3 (Slc16a3), (10ug)

Product Images

Circular map for MG221679

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc16a3
• Synonyms: Mct3; Mct4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221679 representing NM_001038653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGGCTGTGGTGGACGAGGGTCCCACCGGCATCAAGGCCCCCGATGGGGGCTGGGGCTGGGCCG
  TCCTCTTTGGCTGTTTTATCATCACGGGTTTCTCCTACGCCTTCCCCAAGGCGGTCAGCGTCTTTTTCAA
  GGAGCTTATGCATGAGTTTGGGATTGGCTACAGCGACACGGCTTGGATCTCCTCCATCCTGCTGGCTATG
  CTCTATGGCACAGGACCGCTCTGCAGTGTGTGTGTGAACCGCTTTGGCTGCCGGCCTGTCATGCTTGTGG
  GTGGCCTCTTTGCGTCCCTGGGAATGGTGGCTGCGTCCTTCTGCAGAAGCATTATCCAGATCTACCTCAC
  CACGGGGGTCATCACTGGCTTGGGTCTGGCTCTCAACTTCCAGCCTTCCCTCATCATGCTCAATCGATAC
  TTCAACAAGCGTCGCCCTATTGCCAATGGGTTGGCAGCGGCAGGCAGCCCAGTGTTCCTTTGTGCACTGT
  CTCCACTGGGGCAGCTGCTGCAGGATCACTATGGTTGGCGGGGTGGCTTCCTCATCCTGGGGGGCCTACT
  GCTCAACTGCTGTGTGTGTGCTGCGCTCATGAGGCCGCTGGTGGCACCGCAGGTAGGCGGAGGGACTGAG
  CCCCGCGGACCCCAGCGGCCACCTCAACGCCTGCTAGACCTGAGTGTCTTCCGAGACCGCGGCTTCCTCA
  TCTATGCCGTGGCCGCCTCAATCATGGTGCTGGGACTCTTTGTGCCTCCGGTCTTTGTGGTGAGCTATGC
  TAAGGATATGGGTGTACCCGACACAAAGGCCGCCTTCCTTCTCACCATCCTGGGCTTCATCGACATCTTT
  GCCAGGCCCACGGCTGGTTTCATAACAGGCCTCAAGAAGGTGCGGCCCTACTCTGTCTACCTCTTCAGCT
  TTGCCATGTTCTTCAATGGCTTCACTGACCTGACAGGCTCCACAGCCACTGACTACGGTGGCCTGGTGGT
  CTTTTGCATCTTCTTTGGCATCTCATATGGCATGGTGGGCGCACTACAGTTTGAGGTGCTCATGGCTATC
  GTGGGCACTCAGAAGTTCTCCAGTGCCATTGGTCTCGTGCTGCTGCTGGAGGCCGTGGCTGTGCTCATCG
  GACCCCCGTCAGGAGGCAAGCTGCTGGATGCAACCAAAGTTTACAAGTATGTGTTCATCCTGGCGGGGGC
  CGAGGTGCTCACCTCCTCCCTTGTGCTGCTGCTGGGCAACTTCTTCTGCATCGGGAAGAGGAAGAGGCCC
  GAGGTGACAGAGCCTGAGGAGGTGGCCTCAGAGGAGAAGCTCCACAAGCCCCCTGTGGACGTGGGAGTGG
  ACTCGAGGGAGGTGGAGCACTTCCTGAAGGCAGAGCCTGAGAAAAATGGGGAGGTGGTTCATACCCCGGA
  AACCAGCGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221679 representing NM_001038653
  Red=Cloning site Green=Tags(s)
  
  MGGAVVDEGPTGIKAPDGGWGWAVLFGCFIITGFSYAFPKAVSVFFKELMHEFGIGYSDTAWISSILLAM
  LYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQIYLTTGVITGLGLALNFQPSLIMLNRY
  FNKRRPIANGLAAAGSPVFLCALSPLGQLLQDHYGWRGGFLILGGLLLNCCVCAALMRPLVAPQVGGGTE
  PRGPQRPPQRLLDLSVFRDRGFLIYAVAASIMVLGLFVPPVFVVSYAKDMGVPDTKAAFLLTILGFIDIF
  ARPTAGFITGLKKVRPYSVYLFSFAMFFNGFTDLTGSTATDYGGLVVFCIFFGISYGMVGALQFEVLMAI
  VGTQKFSSAIGLVLLLEAVAVLIGPPSGGKLLDATKVYKYVFILAGAEVLTSSLVLLLGNFFCIGKRKRP
  EVTEPEEVASEEKLHKPPVDVGVDSREVEHFLKAEPEKNGEVVHTPETSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001038653
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038653.1, NP_001033742.1
• RefSeq Size: 2481 bp
• RefSeq ORF: 1413 bp
• Locus ID: 80879
• UniProt ID: P57787
• Cytogenetics: 11 E2
• Gene Summary: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).[UniProtKB/Swiss-Prot Function]