Akap14 (NM_001033785) Mouse Tagged ORF Clone
CAT#: MG221217
- TrueORF®
Akap14 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 14 (Akap14), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001033785" in other vectors (2)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Akap14 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG221217 representing NM_001033785, codon optimized.
Due to the complexity of NM_001033785, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAACAAAAAAAAATTCCCCAATCAGAAAGCAAAGATCGTTGAAAACACATCAACCGCCAAGACTA AAAAACCCGAAGCACCCGAACCCAAACATGTTACTGAGCCTAAGGACTCAAAATCCGTGGAGGATCCCAA GATTCCTGTCACCGACCAGAAGGAGAAGAAGACGGTAACTACCATTGCGGAGCCCAAGGACAAGAAGTCC GAAACCGAACCCAAAGAGAAGAAGACTGTGACCATATCTGACCCGAAAGCCAAGAAGCCAGCGATCGAGA AGGACAAACGAACGGTCACATTCGTGACCGAGGTAAAAGACAAGAGGATCGTTACGGATACCAAGGAGAA TCGCATCGCCTCCATCGACACAAAGGAAAAGGTGCCAGTAGTGGTGCCCGAAGTGAAGGAGAAGCGGGTG CAGCTGTTGGATCCAAAGGAGAAGGTCGCGGTGCTGCCTACAACACCAATCGGTTCCGTGGCCCATGTCC CTCACGCACCACCTGTCGCACACACTGAGCATGCAGCCCCCGCTAAGCCTGTGGCCCCGGCTAAGCCCGT CGCCCCCGTGGCACCTGCCAAACCCGTCGCTCCAGTGGCACCCGCTAAGCCTGTCGTCCCTGTGGCCCCT GCCAAACCCGTCGCACCTGCCGCCCCTGTCACATCAGTAGTCAGAGCCGTGCCAGCCGCACCAGTGACTC CAGTGGCTCCAGTTGCTCCAGCTGTACCAGTGGTGCCAGCTGCCCCTGTTGCACCAGCCGCACCCGTCGT GCCCGCCGCTCCCGTCGCTCCCGTAGCCCCAGCCCTTCCCGTGGCTCCTGTGGCGCCGGTAGCCCCAGCT CTGCCCGTGGCCCCTGCCGTCCCCGTGGCTCCCGCCGTGCCTGTCGCCCCAGTCGTGCCGGAGGTAAAGC AGAAAATCCCCGTCTCCGAGGTCAAGGGTAAGAAGGAGGTGCCCCTCCGCGTCCCTGAACTTAAGGAAAT CAAAGCAAGCTCTTTTGCCCCGGAAATTAAAGTGAAAAAAGTTGGCAGTGAAATTAAGCAAAAGGCTCAC GTCAAAGATGTCAAAGAGAAGAAAATTGTGCCTGAGGTTAAGGAGAAGAAGCCGGTCGTGGTTAAGAAAA AGGACGGTGCATCTGAGGTGGCTCGGACTGTGGTGGATGGGGTGATGGCCGCCGCAGTAGAGATGGTGGA AGAAGCTCGCAACCCAATAAAAAATATTAAGTGGTTGACCCACGGCGAATTCACTCCTGAGAAAGGAAGG AAGCAGATCGAAAAGTTCGTGTCTACATGGGAATTTCAGAACCGGTGGGTGTACTATGCAGATTTTATCG AGAAAAAAGACCTGATTCATAGCTATCATTATATATATCGAGTCAGGTGGTCCGCTCCTACTGCAGTGAG GCCTATGGCCCGAGTAAGCGCTAACGCGCTGTTCACAATTAAGTTTAACAAGTCCAAGCCTGCAGATATG CCCGTCGACGTCTCCTACATCTTCGAAAACAGTGAGCTGCTGCAAAGACCCGGTGAGATCAGGTTTCGCG AACAGTGGCTGCGCGACATAACCGAAACGAAGCACATTCTGCTCGAATCTATCCCATTCAAAGTAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221217 representing NM_001033785
Red=Cloning site Green=Tags(s) MSNKKKFPNQKAKIVENTSTAKTKKPEAPEPKHVTEPKDSKSVEDPKIPVTDQKEKKTVTTIAEPKDKKS ETEPKEKKTVTISDPKAKKPAIEKDKRTVTFVTEVKDKRIVTDTKENRIASIDTKEKVPVVVPEVKEKRV QLLDPKEKVAVLPTTPIGSVAHVPHAPPVAHTEHAAPAKPVAPAKPVAPVAPAKPVAPVAPAKPVVPVAP AKPVAPAAPVTSVVRAVPAAPVTPVAPVAPAVPVVPAAPVAPAAPVVPAAPVAPVAPALPVAPVAPVAPA LPVAPAVPVAPAVPVAPVVPEVKQKIPVSEVKGKKEVPLRVPELKEIKASSFAPEIKVKKVGSEIKQKAH VKDVKEKKIVPEVKEKKPVVVKKKDGASEVARTVVDGVMAAAVEMVEEARNPIKNIKWLTHGEFTPEKGR KQIEKFVSTWEFQNRWVYYADFIEKKDLIHSYHYIYRVRWSAPTAVRPMARVSANALFTIKFNKSKPADM PVDVSYIFENSELLQRPGEIRFREQWLRDITETKHILLESIPFKVV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033785 |
ORF Size | 1608 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033785.2, NP_001028957.1 |
RefSeq Size | 1792 bp |
RefSeq ORF | 1611 bp |
Locus ID | 434756 |
Cytogenetics | X A3.3 |
Gene Summary | This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The human ortholog of this gene has been characterized. It is reported to be enriched in airway cilia and to regulate ciliary beat frequency by anchoring the protein kinase A holoenzyme near substrates in the axoneme. [provided by RefSeq, May 2013] |
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