Akap14 (NM_001033785) Mouse Tagged ORF Clone

CAT#: MG221217

Akap14 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 14 (Akap14), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG221217

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Akap14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221217 representing NM_001033785, codon optimized.
  Due to the complexity of NM_001033785, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAACAAAAAAAAATTCCCCAATCAGAAAGCAAAGATCGTTGAAAACACATCAACCGCCAAGACTA
  AAAAACCCGAAGCACCCGAACCCAAACATGTTACTGAGCCTAAGGACTCAAAATCCGTGGAGGATCCCAA
  GATTCCTGTCACCGACCAGAAGGAGAAGAAGACGGTAACTACCATTGCGGAGCCCAAGGACAAGAAGTCC
  GAAACCGAACCCAAAGAGAAGAAGACTGTGACCATATCTGACCCGAAAGCCAAGAAGCCAGCGATCGAGA
  AGGACAAACGAACGGTCACATTCGTGACCGAGGTAAAAGACAAGAGGATCGTTACGGATACCAAGGAGAA
  TCGCATCGCCTCCATCGACACAAAGGAAAAGGTGCCAGTAGTGGTGCCCGAAGTGAAGGAGAAGCGGGTG
  CAGCTGTTGGATCCAAAGGAGAAGGTCGCGGTGCTGCCTACAACACCAATCGGTTCCGTGGCCCATGTCC
  CTCACGCACCACCTGTCGCACACACTGAGCATGCAGCCCCCGCTAAGCCTGTGGCCCCGGCTAAGCCCGT
  CGCCCCCGTGGCACCTGCCAAACCCGTCGCTCCAGTGGCACCCGCTAAGCCTGTCGTCCCTGTGGCCCCT
  GCCAAACCCGTCGCACCTGCCGCCCCTGTCACATCAGTAGTCAGAGCCGTGCCAGCCGCACCAGTGACTC
  CAGTGGCTCCAGTTGCTCCAGCTGTACCAGTGGTGCCAGCTGCCCCTGTTGCACCAGCCGCACCCGTCGT
  GCCCGCCGCTCCCGTCGCTCCCGTAGCCCCAGCCCTTCCCGTGGCTCCTGTGGCGCCGGTAGCCCCAGCT
  CTGCCCGTGGCCCCTGCCGTCCCCGTGGCTCCCGCCGTGCCTGTCGCCCCAGTCGTGCCGGAGGTAAAGC
  AGAAAATCCCCGTCTCCGAGGTCAAGGGTAAGAAGGAGGTGCCCCTCCGCGTCCCTGAACTTAAGGAAAT
  CAAAGCAAGCTCTTTTGCCCCGGAAATTAAAGTGAAAAAAGTTGGCAGTGAAATTAAGCAAAAGGCTCAC
  GTCAAAGATGTCAAAGAGAAGAAAATTGTGCCTGAGGTTAAGGAGAAGAAGCCGGTCGTGGTTAAGAAAA
  AGGACGGTGCATCTGAGGTGGCTCGGACTGTGGTGGATGGGGTGATGGCCGCCGCAGTAGAGATGGTGGA
  AGAAGCTCGCAACCCAATAAAAAATATTAAGTGGTTGACCCACGGCGAATTCACTCCTGAGAAAGGAAGG
  AAGCAGATCGAAAAGTTCGTGTCTACATGGGAATTTCAGAACCGGTGGGTGTACTATGCAGATTTTATCG
  AGAAAAAAGACCTGATTCATAGCTATCATTATATATATCGAGTCAGGTGGTCCGCTCCTACTGCAGTGAG
  GCCTATGGCCCGAGTAAGCGCTAACGCGCTGTTCACAATTAAGTTTAACAAGTCCAAGCCTGCAGATATG
  CCCGTCGACGTCTCCTACATCTTCGAAAACAGTGAGCTGCTGCAAAGACCCGGTGAGATCAGGTTTCGCG
  AACAGTGGCTGCGCGACATAACCGAAACGAAGCACATTCTGCTCGAATCTATCCCATTCAAAGTAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221217 representing NM_001033785
  Red=Cloning site Green=Tags(s)
  
  MSNKKKFPNQKAKIVENTSTAKTKKPEAPEPKHVTEPKDSKSVEDPKIPVTDQKEKKTVTTIAEPKDKKS
  ETEPKEKKTVTISDPKAKKPAIEKDKRTVTFVTEVKDKRIVTDTKENRIASIDTKEKVPVVVPEVKEKRV
  QLLDPKEKVAVLPTTPIGSVAHVPHAPPVAHTEHAAPAKPVAPAKPVAPVAPAKPVAPVAPAKPVVPVAP
  AKPVAPAAPVTSVVRAVPAAPVTPVAPVAPAVPVVPAAPVAPAAPVVPAAPVAPVAPALPVAPVAPVAPA
  LPVAPAVPVAPAVPVAPVVPEVKQKIPVSEVKGKKEVPLRVPELKEIKASSFAPEIKVKKVGSEIKQKAH
  VKDVKEKKIVPEVKEKKPVVVKKKDGASEVARTVVDGVMAAAVEMVEEARNPIKNIKWLTHGEFTPEKGR
  KQIEKFVSTWEFQNRWVYYADFIEKKDLIHSYHYIYRVRWSAPTAVRPMARVSANALFTIKFNKSKPADM
  PVDVSYIFENSELLQRPGEIRFREQWLRDITETKHILLESIPFKVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033785
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033785.2, NP_001028957.1
• RefSeq Size: 1792 bp
• RefSeq ORF: 1611 bp
• Locus ID: 434756
• Cytogenetics: X A3.3
• Gene Summary: This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The human ortholog of this gene has been characterized. It is reported to be enriched in airway cilia and to regulate ciliary beat frequency by anchoring the protein kinase A holoenzyme near substrates in the axoneme. [provided by RefSeq, May 2013]