Rin3 (NM_177620) Mouse Tagged ORF Clone

CAT#: MG221158

Rin3 (tGFP-tagged) - Mouse Ras and Rab interactor 3 (Rin3) transcript variant 1, (10ug)

Product Images

Circular map for MG221158

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rin3
• Synonyms: 6430500K07; C86933
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221158 representing NM_177620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACGCGCCGAAGCGCCCTCGAGCGCCCACCCTGCCGGTCCTATTCCAGATGCTGGCAAAGGAGAGG
  GAGAGGAAGATGAGGAGAAAGACGGCACGAGGCTTGGTCTGTCAACCACCCCGAGGAACTGCATCCCACG
  CCGGGGCATCAGTGTCCTGGAGAAGCTGGTGAAGACCTGCCCTGTGTGGCTGCAGCTAGGGCTAGGCCAG
  GCAGAGGCTGCCAAGATCCTGCAGCAGGAGATGGCTGGGATGTTCCTGGTGTGCCGGGACAACAACCTGA
  AGCAATTGGTGCTGTGTGTCCACTTCCCTTCCCTGAAGGGCAGCTCAGCTGAGGTGCTGGAGTACCCCAT
  CAAAGAAGAAAAGGCTATACTGTACCTGGAAGGCTCAGTTCTTGTGTTTGAGGACATCTTCAGACTGATT
  GCGTTCTACTGTGTCAGTAGAGACTTACTGCCCTTCACTCTACGGCTGCCCCAGGCCATCCTCGAAGCTA
  GCAGCTTCCTTGAACTTGAGACCATCTCCAACCTGGGCCTGGGTTTCTGGGACTCCTCGCTGAACTCAAG
  AGGCTCTGCAGAGCCACTCAGAAGCCCAGCCCCTGGGACCCCTGCATCCTCCAGCCTCCGGCCTACAACC
  CATTACGCAAATTGTTCCTGTGAAATCGAGCTCTCCGTGGGAAATGACCGCCTGTGGTTTGTGAATCCTA
  TTTTCATCGAGGACTGCATCCTACCGGCTGATCCACCTCCACTGCCTACTGGCAGCTACCCTCCACGCCC
  TACGCCTGCCACCCCGGATGCTACCTCACCCACCTCCAAGGGATCCCCACGTCGCCCCCCACCTCCACCC
  CCGTTACCCACTGTTCCTCCTACTGGCCCTGCACGGCCTCTTGCGCCTCCTGTACCGCCTGCTGGCCCTT
  TGCCCAACTCCCCCCTAACGCCCACCTCTCACCTTGCACCCCATGCACCGGGCCCCCCTGGCCACTCAAA
  CCAACCACCCATGACAGCCTGCGAGAGTCTCCCTCGCCCAGCTGTAGGCCTGGGCCCCTTTGGAGAAGAA
  GAGATGAAACCTGGGACAACTCCCAATCCCTTGCACCAAGCCCCTCCCCCTCCATTACCCCTGAAGAAGG
  CTCTTCCAGCAGCCCCTCCCAGGAGGCGCATTTCTGAGAGGGTATCTCTGGAAAGCCAGAATGTGGGGAC
  TTCAACAGACAGGGACCACTCAGGCATTTCTAGGACAGCATCACTCAACCTTCCTCCCCAGAGCACCGTA
  AGTAGCCTTGGGGACAGGCCTCCAAGGACCACGGAGCAAAGCCAGGACACAGAGGCCAAAGCCAGCCATG
  CTGACAGCATACCAGTGCCTCCTGGGAAGGCCAAACAACCCCCAGTACCACCCCCAAGGAAGAAGCGGGT
  CTCCCGGCAGCTGGCCTCAACCCTTCTCAGTCCCTTGGAGAGCCCCATACAGGAGGCATCCTCTGAGAAA
  CAGGCGACTGGTGCATCTTGGGAAGGCTTGAGTCCTGTCCGGCAAGCTGGCATGCAGCACCTCCAAGTTC
  AGTCCAGCAGCTGTCCCCAAAGCTCTCCAGAGTTCAAGGGTTCCCAGGCCTCACTCTCAGACAGCCTGGG
  GGTGCCTGCCTCGGCCGCTGACCAGGACTCCTACTCAACCAGCAGTGCAGAGGAGGAGCTGGAGTTCAGC
  AGCCCCAACGTGAAGAAGAAACCCTCAATGATCCTGGACAAGGCCCGCCACCGCCTCAGCTTCGTCAGCT
  TCGCCAGCGTCTTCCATGCCTTCCTCTCCAGCGACCGCAAACTGTATAAGAAGGTGGTGGAGCTGGCACA
  GGACAAGAGCTCCTACTTCGGCAGCCTGGTGCAGGACTACAAGGTGTACAGCCTGGAGATGATGGCCCGC
  CAGACATCCAGCACTGAGATGCTGCAGGAGATCCGTACCATGATGACCCAGCTCAAGAGCTACCTGCTAC
  AGAGCACTGAACTCAAAGCCTTGGTGGAACCCACCCTGCACTCGGAGGAGGAACTCGAGGCCATAGTGGA
  GTCAGCCTTGTACAAGTGTGTCTTGAAGCCCCTGAAGGAGGCCATCAACTCCAGCCTGCTGGAGATCCAC
  AGCCGGGATGGCTCACTGCAGCAGCTCAAGGAGAACCAGCTAGTGGTCCTGGCCACCACCACCACCGACC
  TGGGTGTGACCACCAGCGTGCCTGAGGTGGCTGTCATGGAGAAGATCCTGCAGAAGCTGACCAGCATGCA
  CAAAGCCTACTCACCAGGGAAGAAAATCTCCATCTTGCTGAAGACCTGCAAGCTCATCTATGACTCCATG
  GCTCTTGGCAACCCAGGGAAGCCCTACGGGGCCGACGACTTCCTGCCTGTGCTCATGTACGTGCTGGCCC
  GCAGCAACCTCACTGAGATGCTCCTCAACGTGGAGTACATGATGGAGCTCATGGACCCCGCCCTGCAGTT
  AGGAGAGGGTTCCTACTATCTGACCACCACCTACGGAGCCCTGGAGCACATTAAAAACTATGACAAGATC
  ACAGTGACCCGACAGCTGAGCGTGGAGGTGCAGGACTCCATCCATCGCTGGGAACGCAGGCGCACGCTCA
  ACAAAGCGCGGGCCTCCCGCTCCTCTGTGCAGGACTTCATCTGTGTGTCCTACCTGAAGCCCGAGCAGCA
  GTCACGGACACTGGCGTCACGGGCAGACACAGCAGCCCAGGCACTGTGTGCACAGTGCGCTGAGAAGTTT
  GAGGTGTCACAGCCCCAAGACTACCGGCTCTTCGTGCTGGTGGACGGGCGCTGCTTCCAGCTGGCCGACG
  AGGCTCTGCCGCATCGCATCAAGGGTTATCTGCTTCGGAGCGAGCCCAAACGAGACTTCCACTTCGTGTA
  CCGGCCCCAGGACAGCGGCAAGGATGCTTCAAGCCAGCCCTGTATAGTAGTGCGGGAACCCAACTTCCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221158 representing NM_177620
  Red=Cloning site Green=Tags(s)
  
  MRRAEAPSSAHPAGPIPDAGKGEGEEDEEKDGTRLGLSTTPRNCIPRRGISVLEKLVKTCPVWLQLGLGQ
  AEAAKILQQEMAGMFLVCRDNNLKQLVLCVHFPSLKGSSAEVLEYPIKEEKAILYLEGSVLVFEDIFRLI
  AFYCVSRDLLPFTLRLPQAILEASSFLELETISNLGLGFWDSSLNSRGSAEPLRSPAPGTPASSSLRPTT
  HYANCSCEIELSVGNDRLWFVNPIFIEDCILPADPPPLPTGSYPPRPTPATPDATSPTSKGSPRRPPPPP
  PLPTVPPTGPARPLAPPVPPAGPLPNSPLTPTSHLAPHAPGPPGHSNQPPMTACESLPRPAVGLGPFGEE
  EMKPGTTPNPLHQAPPPPLPLKKALPAAPPRRRISERVSLESQNVGTSTDRDHSGISRTASLNLPPQSTV
  SSLGDRPPRTTEQSQDTEAKASHADSIPVPPGKAKQPPVPPPRKKRVSRQLASTLLSPLESPIQEASSEK
  QATGASWEGLSPVRQAGMQHLQVQSSSCPQSSPEFKGSQASLSDSLGVPASAADQDSYSTSSAEEELEFS
  SPNVKKKPSMILDKARHRLSFVSFASVFHAFLSSDRKLYKKVVELAQDKSSYFGSLVQDYKVYSLEMMAR
  QTSSTEMLQEIRTMMTQLKSYLLQSTELKALVEPTLHSEEELEAIVESALYKCVLKPLKEAINSSLLEIH
  SRDGSLQQLKENQLVVLATTTTDLGVTTSVPEVAVMEKILQKLTSMHKAYSPGKKISILLKTCKLIYDSM
  ALGNPGKPYGADDFLPVLMYVLARSNLTEMLLNVEYMMELMDPALQLGEGSYYLTTTYGALEHIKNYDKI
  TVTRQLSVEVQDSIHRWERRRTLNKARASRSSVQDFICVSYLKPEQQSRTLASRADTAAQALCAQCAEKF
  EVSQPQDYRLFVLVDGRCFQLADEALPHRIKGYLLRSEPKRDFHFVYRPQDSGKDASSQPCIVVREPNFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177620
• ORF Size: 2940 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177620.4, NP_808288.2
• RefSeq Size: 3880 bp
• RefSeq ORF: 2943 bp
• Locus ID: 217835
• UniProt ID: P59729
• Cytogenetics: 12 E
• Gene Summary: Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function (By similarity).[UniProtKB/Swiss-Prot Function]