Gas7 (NM_008088) Mouse Tagged ORF Clone

CAT#: MG220917

Gas7 (tGFP-tagged) - Mouse growth arrest specific 7 (Gas7) transcript variant 1, (10ug)

Product Images

Circular map for MG220917

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gas7
• Synonyms: AW124766; B230343A10Rik; Gas7-cb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220917 representing NM_008088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACGGCACTTCAGAAGCCTGGGATGGTCCCCCCTCCGCCAGGAGAGGAGAGCCAGACGGTCATCC
  TTCCACCCGGCTGGCACAGCTACTTGTCTCCTCAGGGCCGCCGTTACTATGTCAACACGACTACCAATGA
  GACCACCTGGGAACGTCCCAGCAGTTCTCCTGGGATTTCAGCCAGCCCTGGCCCTCACAGGAGCTCTTTG
  CCTACAACAGTGAATGGATACCACGCATCAGGGACCCCGGCGCACCCTCCAGAGACTGCCCACATGAGTC
  TCCGAAAATCCACCGGTGATTCCCAGAACCTGGGATCCTCATCGCCAGGCAGGAAACAGAGCAAGGAAAA
  CACCATTACCATCAACTGTGTGACATTCCCTCACCCGGACACGATGCCTGAGCAACAGCTGCTCAAGCCC
  ACCGAGTGGAGCTACTGTGACTACTTTTGGGCGGACAAGAAGGACCCACAAGGCAATGGCACGGTGGCTG
  GCTTTGAACTGCTGCTGCAGAAGCAACTGAAAGGCAAGCAGATGCAGAAGGAGATGTCCGAGTTCATCCG
  GGAAAGGATAAAGATTGAAGAAGAATATGCAAAGAACCTGGCTAAGCTCTCTCAGAACTCCTTGGCTGCC
  CAGGAGGAAGGCTCCTTGGGAGAGGCATGGGCCCAAGTAAAGAAGAGCCTGGCAGATGAGGCTGAAGTTC
  ATCTCAAGTTCTCTGCCAAGCTCCACAGCGAGGTGGAGAAACCCCTGATGAACTTCAGAGAGAACTTCAA
  GAAAGACATGAAAAAGTGCGACCACCACATCGCTGACCTCCGTAAGCAGCTGGCGAGCCGCTATGCCTCA
  GTGGAGAAGGCCCGCAAAGCCCTCACAGAGCGGCAGAAAGACCTGGAGATGAAGACCCAGCAGCTGGAGA
  TCAAGTTGAGCAACAAGACCGAGGAGGACATCAAGAAGGCACGGAGGAAGTCCACTCAGGCCGGAGATGA
  CCTCATGCGCTGTGTGGACCTCTACAACCAGGCCCAGTCCAAGTGGTTTGAAGAGATGGTGACGACCACA
  CTGGAACTGGAGCGGCTGGAGGTGGAGAGGGTGGAAATGATCCGACAACATCTGTGCCAGTACACACAGC
  TGCGGCACGAGACGGACATGTTCAACCAAAGCACAGTCGAGCCTGTGGACCAACTGCTGCGAAAAGTGGA
  CCCAGCCAAAGACAGAGAGCTATGGGTCAGAGAGCACAAAACAGGCAACATCCGCCCAGTGGACATGGAG
  ATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220917 representing NM_008088
  Red=Cloning site Green=Tags(s)
  
  MATALQKPGMVPPPPGEESQTVILPPGWHSYLSPQGRRYYVNTTTNETTWERPSSSPGISASPGPHRSSL
  PTTVNGYHASGTPAHPPETAHMSLRKSTGDSQNLGSSSPGRKQSKENTITINCVTFPHPDTMPEQQLLKP
  TEWSYCDYFWADKKDPQGNGTVAGFELLLQKQLKGKQMQKEMSEFIRERIKIEEEYAKNLAKLSQNSLAA
  QEEGSLGEAWAQVKKSLADEAEVHLKFSAKLHSEVEKPLMNFRENFKKDMKKCDHHIADLRKQLASRYAS
  VEKARKALTERQKDLEMKTQQLEIKLSNKTEEDIKKARRKSTQAGDDLMRCVDLYNQAQSKWFEEMVTTT
  LELERLEVERVEMIRQHLCQYTQLRHETDMFNQSTVEPVDQLLRKVDPAKDRELWVREHKTGNIRPVDME
  I
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008088
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008088.4, NP_032114.2
• RefSeq Size: 6954 bp
• RefSeq ORF: 1266 bp
• Locus ID: 14457
• Cytogenetics: 11 B3